A5057818048- Gestational Diabetes Research and Management
- Birth, Development, and Health
- Hormonal Regulation and Hypertension
- Pregnancy and preeclampsia studies
- Nitric Oxide and Endothelin Effects
- Renin-Angiotensin System Studies
- Neuropeptides and Animal Physiology
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Apelin-related biomedical research
- Peptidase Inhibition and Analysis
- Cardiovascular Health and Disease Prevention
- Traumatic Brain Injury and Neurovascular Disturbances
- Bariatric Surgery and Outcomes
- Blood Pressure and Hypertension Studies
- Neuroscience of respiration and sleep
- Advanced Glycation End Products research
- Eicosanoids and Hypertension Pharmacology
- Heart Rate Variability and Autonomic Control
- Systemic Lupus Erythematosus Research
- Body Contouring and Surgery
- Connective tissue disorders research
- Aortic aneurysm repair treatments
- Angiogenesis and VEGF in Cancer
- Nuclear Receptors and Signaling
- Atherosclerosis and Cardiovascular Diseases
Université d'Angers
2014-2025
Inserm
2015-2025
Centre National de la Recherche Scientifique
2014-2025
Laboratoires d'Anjou (France)
2020
Centre de Recherche des Cordeliers
2008-2014
Sorbonne Université
2008-2014
Université Paris Cité
2006-2013
Laboratoire de Neurobiologie Cellulaire et Moléculaire
2013
Essen University Hospital
2012
Délégation Centre-Est
2012
Pulse pressure is a stronger predictor of cardiovascular events than systolic or diastolic blood in large cohorts French and North American patients. However, its influence on stroke controversial. Large-artery stiffness the main determinant pulse pressure. The arterial occurrence has never been demonstrated. Our aim was to establish relationship between aortic death hypertensive patients.We included, longitudinal study, 1715 essential patients who had measurement at entry (ie, 1980 2001) no...
Liddle syndrome, an inherited form of hypertension, is caused by gain-of-function mutations in the epithelial Na(+) channel (ENaC), principal mediator reabsorption kidney. Accordingly, disease pathology was ascribed to a primary renal mechanism. Whether this sole responsible mechanism, however, remains uncertain as dysregulation ENaC other tissues may also be involved. Previous work indicates that vascular endothelium crucial for regulation cellular mechanics and thus function. The hormone...
Abstract Obesity is a major public health issue worldwide. Despite various approaches to weight loss, the most effective technique for reducing obesity, as well diabetes and associated diseases, bariatric surgery. Increasingly, young women without children are undergoing surgery, vertical sleeve gastrectomy (VSG) being common procedure nowadays. However, despite several reports suggesting bone loss after VSG, little known about potential additive effects of gestation lactation VSG health....
Abstract Apelin is a novel neuropeptide involved in the regulation of body fluid homeostasis and cardiovascular functions. It acts through G protein‐coupled receptor, APJ receptor. We studied structure–activity relationships apelin at rat tagged its C‐terminal end with enhanced green fluorescent protein stably expressed CHO cells. evaluated potency N‐ deleted fragments K17F to bind high affinity inhibit cAMP production induce receptor internalization. first characterized internalization...
The hyperactivity of the brain renin-angiotensin system (RAS) has been implicated in development and maintenance hypertension several types experimental genetic animal models. We previously reported that murine brain, aminopeptidase A (APA) is involved conversion angiotensin II (AngII) to AngIII one main effector peptides RAS control vasopressin release. Here we report exerts a tonic stimulatory effect on blood pressure model salt-dependent hypertension, DOCA-salt rat, characterized by...
Overactivity of the brain renin-angiotensin system has been implicated in development and maintenance hypertension. We reported previously that angiotensin II is converted to III by aminopeptidase A mouse brain. then used specific selective inhibitors show one main effector peptides system, exerting tonic stimulatory control over blood pressure hypertensive rats. Aminopeptidase A, enzyme generating III, thus represents a potential candidate central nervous target for treatment Given this...
Epidemiological and experimental studies have led to the hypothesis of fetal origin adult diseases, suggesting that some diseases might be determined before birth by altered development. We previously demonstrated in rat utero exposure maternal diabetes impairs renal development leading a reduction nephron number. Little is known on long-term consequences diabetes. The aim study was assess, rat, effects blood pressure function adulthood.Diabetes induced Sprague-Dawley pregnant rats...
The Epithelial Sodium Channel (ENaC) is a key player in renal sodium homeostasis. expression of α β γ ENaC subunits has also been described the endothelium and vascular smooth muscle, suggesting role function. We recently demonstrated that endothelial involved aldosterone-modulated stiffness. Here we explore functional αENaC subunit function vivo. Compared to littermates, mice with conditional gene inactivation only (endo-αENaC Knock Out mice) had no difference their physiological parameters...
Previous genomic studies with human tissues have compared differential gene expression between 2 conditions (ie, normal versus diseased) to identify altered in a binary manner; however, potentially more informative approach is correlate the levels of quantitative physiological parameters.In this study, we used examine genes whose correlates arterial stiffness aortic specimens. Our data distinct groups genes, those associated cell signaling and mechanical regulation vascular structure...
Flow-mediated remodeling of resistance arteries is essential for revascularization in ischemic diseases, but this impaired diabetes. We hypothesized that breaking advanced glycation end product (AGE) cross-links could improve mesenteric Zucker diabetic fatty (ZDF) rats compared with lean (LZ) rats. Arteries, exposed to high (HF) or normal (NF) blood flow after alternate arterial ligation vivo, were collected 2 weeks. In LZ rats, HF artery diameter was larger than NF vessels, not the case ZDF...
Hypertension is associated with excessive reactive oxygen species (ROS) production in vascular cells. Mitochondria undergo fusion and fission, a process playing role mitochondrial function. OPA1 essential for fusion. Loss of ROS cell dysfunction. We hypothesized that mitochondria could reduce oxidative stress defect would exacerbate hypertension. Using (a) Opa1 haploinsufficiency isolated resistance arteries from Opa1+/− mice, (b) primary cells (c) RNA interference experiments siRNA against...
Postnatal overfeeding (OF) in rodents induces a permanent moderate increase body weight adulthood. However, the repercussions of postnatal OF on cardiac gene expression, metabolism and nitro-oxidative stress are less well known.Immediately after birth, litters C57BL/6 mice were either maintained at 10 (normal-fed group, NF), or reduced to 3 order induce OF. At weaning, both groups received standard diet. The expression profile was determined weaning oxidative assessed 7 months. several...
Pathophysiological aldosterone (aldo)/mineralocorticoid receptor signaling has a major impact on the cardiovascular system, resulting in hypertension and vascular remodeling. Mineralocorticoids induce endothelial dysfunction, decreasing vasorelaxation response to acetylcholine increasing vasoconstrictors. Activation of epidermal growth factor (EGFR) is thought mediate effects aldo, but this yet be demonstrated vivo. In study, we analyzed molecular functional consequences aldo-salt challenge...
Endothelial dysfunction in resistance arteries alters end organ perfusion type 2 diabetes. Superoxides and cyclooxygenase-2 (COX-2) derivatives have been shown separately to alter endothelium-mediated relaxation aging diabetes but their role the alteration of vascular tone old diabetic subjects is not clear, especially arteries. Consequently, we investigated superoxide COX-2-derivatives on endothelium-dependent 3 12 month-old Zucker fatty (ZDF) lean (LZ) rats. Mesenteric were isolated was...
Previous studies have shown that aldosterone, which activates the mineralocorticoid receptor (MR), promotes thrombosis in animal models. Our objective was to determine whether MR activation/expression vascular endothelium could modify thrombotic risk vivo and examine thrombin generation at surface of aortic endothelial cells (HAECs). conditionally overexpressed mice (MR-EC mice) or stimulated with aldosterone HAECs. Thrombosis after ferric chloride injury delayed MR-EC compared controls as...
The endothelium is the production site of several potent vasoactive factors that contribute to modulation vascular tone. Because hemoglobin-based oxygen carriers (HBOC) have been demonstrated cause vasoconstriction and thereby increase arterial pressure by interacting with endothelium-derived such as nitric oxide endothelin-1, we hypothesized hemoglobin could penetrate into endothelial cells. Therefore, investigated presence guinea pig aortic cells immunohistochemical staining after exchange...
Abstract Aminopeptidase A (APA) generated brain angiotensin III, one of the main effector peptides renin system, exerting a tonic stimulatory effect on control blood pressure in hypertensive rats. The distribution APA human has not been yet studied. We first biochemically characterized (apparent molecular mass 165 and 130 kDa) we showed that enzyme exhibited similar enzymatic characteristics to recombinant mouse APA. Both enzymes had sensitivity Ca 2+ . Kinetic studies K m (190 μmol/L) for...
A chronic increase in blood flow resistance arteries is associated with increased lumen diameter (outward remodeling) and improved endothelium (NO)-mediated relaxation. Flow-mediated remodeling of essential for revascularization ischemic diseases. Nevertheless, it impaired 12 to 24-month old rats young Zucker Diabetic Fatty (ZDF) due advanced glycation end products (AGEs) oxidative stress. As type 2 diabetes occurs preferentially older subjects we investigated flow-mediated the effect AGEs...