A5103130362- Genetic and Kidney Cyst Diseases
- Microtubule and mitosis dynamics
- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Plant and Fungal Interactions Research
- Respiratory viral infections research
- Micro and Nano Robotics
Florida International University
2025
The University of Texas Southwestern Medical Center
2018-2022
Motile cilia are essential for propelling cells and moving fluids across tissues. The activity of axonemal dynein motors must be precisely coordinated to generate ciliary motility, but their regulatory mechanisms not well understood. tether head (T/TH) complex was hypothesized provide mechanical feedback during beating because it links the motor domains I1 doublet microtubule. Combining genetic biochemical approaches with cryoelectron tomography, we identified FAP44 FAP43 (plus...
Nearly all motile cilia contain a central apparatus (CA) composed of two connected singlet microtubules with attached projections that play crucial roles in regulating ciliary motility. Defects CA assembly usually result motility-impaired or paralyzed cilia, which humans causes disease. Despite their importance, the protein composition and functions are largely unknown. Here, we integrated biochemical genetic approaches cryo-electron tomography to compare wild-type Chlamydomonas mutants. We...
In most eukaryotic organisms, cilia and flagella perform a variety of life-sustaining roles related to environmental sensing motility. Cryo-electron microscopy has provided considerable insight into the morphology function flagellar structures, but studies have been limited less than dozen millions known species. Ultrastructural information is particularly lacking for unicellular organisms in Opisthokonta clade, leaving sizeable gap our understanding evolution between species multicellular...
Bacteriophages Biscayne, Bush and GreenIvy were isolated from soil samples in Miami, FL using Microbacterium foliorum NRRL B-24224 as host. Transmission electron microscopy shows siphoviral morphologies for all three phages. Based on gene content similarity to other actinobacteriophages, they are assigned the EE, GA EA5 clusters, respectively.
Axonemal I1 dynein (dynein f) is the largest inner arm in cilia and a key regulator of ciliary beating. It consists two heavy chains, an intermediate chain/light chain (ICLC) complex. However, structural organization nine ICLC subunits remains largely unknown. Here, we used biochemical genetic approaches, cryo-electron tomography imaging Chlamydomonas to dissect molecular architecture Using strain expressing SNAP-tagged IC140, revealed location IC140 N-terminus at proximal apex structure....
Abstract In most eukaryotic organisms, cilia perform a variety of life-sustaining roles related to environmental sensing and motility. Cryo-electron microscopy has provided considerable insight into the morphology function ciliary structures, but studies have been limited less than dozen millions known species. Ultrastructural information is particularly lacking for unicellular organisms in opisthokont clade, leaving sizeable gap our understanding evolution between species multicellular...
Motile cilia have a '9+2' structure containing nine doublet microtubules and central apparatus (CA) composed of two singlet with associated projections. The CA plays crucial roles in regulating ciliary motility. Defects assembly or function usually result motility-impaired paralyzed cilia, which humans causes disease. Despite their importance, the protein composition functions most projections remain largely unknown. Here, we combined genetic, proteomic cryo-electron tomographic approaches...
Abstract Nearly all motile cilia contain a central apparatus (CA) composed of two connected singlet-microtubules with attached projections that play crucial roles in regulating ciliary motility. Defects CA assembly usually result motility-impaired or paralyzed cilia, which humans causes disease. Despite their importance, the protein composition and functions are largely unknown. Here, we integrated biochemical genetic approaches cryo-electron tomography to compare wild type Chlamydomonas...
Abstract ‘9+2’ motile cilia contain 9 doublet microtubules and a central apparatus (CA) composed of two singlet with associated projections. The CA plays crucial roles in regulating ciliary motility. Defects assembly or function usually result motility-impaired paralyzed cilia, which humans causes disease. Despite their importance, the protein composition functions most projections remain largely unknown. Here, we combined genetic approaches quantitative proteomics cryo-electron tomography...