A5037992484- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Gut microbiota and health
- Salmonella and Campylobacter epidemiology
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Celiac Disease Research and Management
- Genomics and Phylogenetic Studies
- Gene expression and cancer classification
- Listeria monocytogenes in Food Safety
- Helicobacter pylori-related gastroenterology studies
- Microbial Metabolites in Food Biotechnology
- Metabolomics and Mass Spectrometry Studies
- Arabic Language Education Studies
- Bacteriophages and microbial interactions
- Identification and Quantification in Food
- Educational Methods and Media Use
- Pharmaceutical and Antibiotic Environmental Impacts
- Digestive system and related health
- Bacterial biofilms and quorum sensing
- Antibiotics Pharmacokinetics and Efficacy
- Cassava research and cyanide
- Food composition and properties
University of Maryland, College Park
2016-2025
Universitas Islam Malang
2017-2025
Universitas Islam Negeri Maulana Malik Ibrahim
2024
Hospital Sultanah Bahiyah
2024
University Kebangsaan Malaysia Medical Centre
2024
Northern University of Malaysia
2023
Universitas Islam Nahdlatul Ulama Jepara
2022
Universitas Negeri Surabaya
2016-2022
Universitas Nahdlatul Ulama Indonesia
2022
Sunan Kalijaga State Islamic University Yogyakarta
2022
The bacteria that colonize humans and our built environments have the potential to influence health. Microbial communities associated with seven families their homes over 6 weeks were assessed, including three moved home. differed substantially among homes, home microbiome was largely sourced from humans. microbiota in each identifiable by family. Network analysis identified as primary bacterial vector, a Bayesian method significantly matched individuals dwellings. Draft genomes of human...
Vibrio cholerae, the causative agent of cholera, is a bacterium autochthonous to aquatic environment, and serious public health threat. V. cholerae serogroup O1 responsible for previous two cholera pandemics, in which classical El Tor biotypes were dominant sixth current seventh respectively. Cholera researchers continually face newly emerging reemerging pathogenic clones carrying diverse combinations phenotypic genotypic properties, significantly hampered control disease. To elucidate...
One of the main challenges in metagenomics is identification microorganisms clinical and environmental samples. While an extensive heterogeneous set computational tools available to classify using whole-genome shotgun sequencing data, comprehensive comparisons these methods are limited. In this study, we use largest-to-date laboratory-generated simulated controls across 846 species evaluate performance 11 metagenomic classifiers. Tools were characterized on basis their ability identify taxa...
Significance The incidence of chronic inflammatory autoimmune conditions, such as celiac disease (CD), is increasing at an alarming rate. CD the only condition for which trigger, gluten, known. However, its etiology and pathogenesis remain incompletely defined recent studies suggest other environmental stimuli may play a key role in pathogenesis. Here, we prospectively examine trajectory gut microbiota starting 18 mo before onset 10 infants who developed did not. We identified alterations...
We report 16S rRNA sequencing of microbiomes associated with organic and conventional retail chickens (n=31), processed via whole-carcass enrichment rinse methodologies. Amplicon was performed targeting the V3-V4 region gene, which resulted Good's coverage exceeding 99.7% libraries.
Vibrio cholerae persists in aquatic environments predominantly a nonculturable state. In this study coccoid, V. O1 biofilms maintained for 495 days Mathbaria, Bangladesh, pond water became culturable upon animal passage. Culturability, biofilm formation, and the wbe, ctxA, rstR2 genes were monitored by culture, direct fluorescent antibody (DFA), multiplex PCR. DFA counts not possible after formation of biofilm. Furthermore, but amplifiable, even incubation 54 68 at room temperature (...
Vibrio parahaemolyticus and vulnificus, which are native to estuaries globally, agents of seafood-borne or wound infections, both potentially fatal. Like all vibrios autochthonous coastal regions, their abundance varies with changes in environmental parameters. Sea surface temperature (SST), sea height (SSH), chlorophyll have been shown be predictors zooplankton thus factors linked vibrio populations. The contribution salinity, conductivity, turbidity, dissolved organic carbon the incidence...
Human saliva is clinically informative of both oral and general health. Since next generation shotgun sequencing (NGS) now widely used to identify quantify bacteria, we investigated the bacterial flora microbiomes two healthy volunteers five datasets from Microbiome Project, along with a control dataset containing short NGS reads species representative human saliva. GENIUS, system designed using unassembled was comprising samples datasets. Results, achieved within minutes at greater than 90%...
The millions of deaths from cholera during the past 200 y, coupled with morbidity and mortality in Haiti since October 2010, are grim reminders that Vibrio cholerae , etiologic agent cholera, remains a scourge. We report isolation both V . O1 non-O1/O139 early epidemic samples collected victims 18 towns across eight Arrondissements Haiti. results showed two distinct populations V. coexisted epidemic. As was sole pathogen isolated 21% clinical specimens, its role this epidemic, either alone...
Cholera outbreak following the earthquake of 2010 in Haiti has reaffirmed that disease is a major public health threat. Vibrio cholerae autochthonous to aquatic environment, hence, it cannot be eradicated but hydroclimatology-based prediction and prevention an achievable goal. Using data from 1800s, we describe uniqueness seasonality mechanism occurrence cholera epidemic regions Asia Latin America. Epidemic are located near regional rivers characterized by sporadic outbreaks, which likely...
Microbiota that co-enrich during efforts to recover pathogens from foodborne outbreaks interfere with efficient detection and recovery. Here, dynamics of co-enriching microbiota recovery Listeria monocytogenes naturally contaminated ice cream samples linked an outbreak are described for three different initial enrichment formulations used by the Food Drug Administration (FDA), International Organization Standardization (ISO), United States Department Agriculture (USDA). Enrichment cultures...
Significance Necrotizing fasciitis (NF) is a rapidly progressing fatal skin and muscle tissue lesion. We studied human case of NF found that the infection was caused by multiple strains A. hydrophila (NF1–NF4). The latter three constitute clonal group, whereas NF1 phylogenetically distinct. tested these individually in mouse intramuscular model observed to be less virulent than NF2. However, when NF2 were mixed, exhibited more virulence it decreased virulence. cross-talk between due presence...
Abstract Blowflies and houseflies are mechanical vectors inhabiting synanthropic environments around the world. They feed breed in fecal decaying organic matter, but microbiome they harbour transport is largely uncharacterized. We sampled 116 individual blowflies from varying habitats on three continents subjected them to high-coverage, whole-genome shotgun sequencing. This allowed for genomic metagenomic analyses of host-associated at species level. Both fly host segregate based principal...
Abstract Recent molecular advances in microbiology have greatly improved the detection of bacterial pathogens environment. These improvements and a downward trend cost methods contributed to increased frequency pathogenic microorganisms where traditional culture‐based failed. Culture also been improved, confluence two suites provides powerful tool for detection, isolation, characterization pathogens. While data on presence type pathogens, culturing allow researcher preserve organism interest...
Abstract Many genomes are incorrectly identified at GenBank. We developed a plan to find and correct misidentified using genomic comparison statistics together with scaffold of reliably from type. A workshop was organized broad representation the bacterial taxonomic community review proposal, GenBank Microbial Genomic Taxonomy Workshop, Bethesda MD, May 12–13, 2015.
Abstract Background Celiac disease (CD) is an autoimmune digestive disorder that occurs in genetically susceptible individuals response to ingesting gluten, a protein found wheat, rye, and barley. Research shows genetic predisposition exposure gluten are necessary but not sufficient trigger the development of CD. This suggests other environmental stimuli early life, e.g., cesarean section delivery antibiotics or formula feeding, may also play key role CD pathogenesis through yet unknown...
The genus Vibrio includes pathogenic species that are naturally occurring in marine and estuarine environments globally. Routine monitoring for environmental parameters influencing their incidence is critical to provide a warning system the public when risk of infection high.
Inflammatory bowel disease (IBD) is increasing among mammals around the world, and domestic dogs are no exception. There approved cure for canine IBD with limited treatment options. Novel probiotic bacteria discovery from free-ranging animals of in pets can likely yield promising candidates. Consequently, overall aim was to isolate that could potentially be utilized as novel probiotics. Two identified unique Paenibacillus spp. strains by small ribosomal RNA (16S) gene sequencing were...
ABSTRACT Since Vibrio cholerae O139 first appeared in 1992, both O1 El Tor and have been recognized as the epidemic serogroups, although their geographic distribution, endemicity, reservoir are not fully understood. To address this lack of information, a study epidemiology ecology V. was carried out two coastal areas, Bakerganj Mathbaria, Bangladesh, where cholera occurs seasonally. The results biweekly clinical (January 2004 to May 2005), employing culture methods, an ecological (monthly...
ABSTRACT The genomes of V ibrio cholerae O1 Matlab variant MJ-1236, Mozambique El Tor B33, and altered CIRS101 were sequenced. All three strains found to belong the phylocore group 1 clade V. , which includes 7th-pandemic serogroup O139 isolates, despite displaying certain characteristics classical biotype. harbor a hybrid CTXΦ an integrative conjugative element (ICE), leading their establishment as successful clinical clones displacement prototypical Tor. absence strain- group-specific...
The treatment regimen for diarrhea depends greatly on correct diagnosis of its etiology. Recent outbreaks in Bangladesh showed Vibrio cholerae to be the predominant cause, although more than 40% suspected cases failed show cholera etiology by conventional culture methods (CMs). In present study, stools collected from every 50th patient during an acute diarrheal outbreak were analyzed extensively using different microbiological and molecular tools determine their Of 135 tested, 86 (64%)...
ABSTRACT Non-O1/non-O139 Vibrio cholerae inhabits estuarine and coastal waters globally, but its clinical significance has not been sufficiently investigated, despite the fact that it associated with septicemia gastroenteritis. The emergence of virulent non-O1/non-O139 V. is consistent recognition new pathogenic variants worldwide. Oyster, sediment, water samples were collected during a vibrio surveillance program carried out from 2009 to 2012 in Chesapeake Bay, Maryland. O1 was detected by...