A5040765860- Cellular transport and secretion
- Lipid Membrane Structure and Behavior
- Erythrocyte Function and Pathophysiology
- Endoplasmic Reticulum Stress and Disease
- Cellular Mechanics and Interactions
- Retinal Development and Disorders
- Advanced biosensing and bioanalysis techniques
- Mitochondrial Function and Pathology
- Ion channel regulation and function
- Extracellular vesicles in disease
- Nanoplatforms for cancer theranostics
- Genetic and Kidney Cyst Diseases
- Glycosylation and Glycoproteins Research
- Calcium signaling and nucleotide metabolism
- Pancreatic function and diabetes
- Microtubule and mitosis dynamics
- Gut microbiota and health
- Fungal and yeast genetics research
- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Chemical Synthesis and Analysis
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Hippo pathway signaling and YAP/TAZ
State Key Laboratory of Supramolecular Structure and Materials
2024
National Taiwan University
2014-2024
Institute of Forensic Science
2024
Shanxi Medical University
2024
Jilin University
2024
Shanghai University
2022-2023
Jiangxi Maternal and Child Health Hospital
2023
Hainan University
2019-2022
Xiamen University
2022
Shandong Normal University
2022
Ferroptosis, a new regulatory cell death modality, underlies the pathogenesis of broad range disorders. Although much efforts have been made to uncover molecular mechanisms, some mechanistic details ferroptosis still remain poorly understood. Particularly, functional relevance mitochondrial reactive oxygen species (ROS) in is highly controversial, which partially due fact that it remains puzzled how ROS level varies during ferroptosis. The conventional mitochondria-targeted probes may react...
Dynamin (Dyn) is a multifunctional GTPase implicated in several cellular events, including endocytosis, intracellular trafficking, cell signaling, and cytokinesis. The mammalian genome encodes three isoforms, Dyn1, Dyn2, Dyn3, splice variants of each, leading to the suggestion that distinct isoforms and/or might mediate functions. We generated conditional Dyn2 KO line performed knockout reconstitution experiments explore isoform- variant specific functions ubiquitously expressed Dyn2. find...
The GTPase dynamin catalyzes the scission of deeply invaginated clathrin-coated pits at plasma membrane, but mechanisms governing dynamin-mediated membrane fission remain poorly understood. Through mutagenesis, we have altered hydrophobic nature membrane-inserting variable loop 1 (VL1) pleckstrin homology (PH) domain dynamin-1 and demonstrate that its stable insertion into lipid bilayer is critical for high curvature generation subsequent fission. Dynamin PH mutants defective in regain...
Dynamin 1 (Dyn1) and Dyn2 are neuronal ubiquitously expressed isoforms, respectively, of the multidomain GTPase required for clathrin-mediated endocytosis (CME). Although they 79% identical, Dyn1 not fully functionally redundant. Through direct measurements basal assembly-stimulated activities, membrane binding, self-assembly, fission on planar curved templates, we have shown that is an efficient curvature generator, whereas primarily a sensor. Using Dyn1/Dyn2 chimeras, identified...
Skeletal muscle requires adequate membrane trafficking and remodeling to maintain its normal structure functions. Consequently, many human myopathies are caused by mutations in machinery. The large GTPase dynamin-2 (Dyn2) is best known for catalyzing fission during clathrin-mediated endocytosis (CME), which critical cell signaling survival. Despite ubiquitous expression, of Dyn2 associated with two tissue-specific congenital disorders: centronuclear myopathy (CNM) Charcot–Marie–Tooth (CMT)...
The main approach for a Wi-Fi indoor positioning system is based on the received signal strength (RSS) measurements, and fingerprinting method utilized to determine user position by matching RSS values with pre-surveyed database. To build fingerprint database essential an system, building such requires lots of time effort. As range environment becomes larger, labor increased. provide better services reduce required establishment at same time, appropriate spatial interpolation needed. In...
Dynamin exhibits a high basal rate of GTP hydrolysis that is enhanced by self-assembly on lipid template. Dynamin's GTPase effector domain (GED) required for this stimulation, though its mechanism action poorly understood. Recent structural work has suggested GED may physically dock with the to exert stimulatory effects. To examine how these interactions activate dynamin, we engineered minimal GTPase-GED fusion protein (GG) reconstitutes dynamin's activity and utilized it define framework...
Significance Membrane asymmetry, curvature, and dynamics have major roles in cellular processes, including vesicle transport. The GTPase ADP ribosylation factor (Arf) a lipid translocase (flippase) are critical for membrane reorganization during formation. Direct evidence that Arf flippase work concert on transformation/architecture is, however, lacking. We demonstrate activated Arf-like protein Arl1 interacts with the Arf-activating guanine nucleotide-exchange Gea2 Drs2, forming ternary...
O-glycosylation is a common protein modification. Aberrant associated with many cancers. GALNT1 GalNAc-transferase that initiates O-glycosylation. We found frequently up-regulated in hepatocellular carcinoma (HCC) and poor patient survival. Overexpression of increased knockdown decreased HCC cell migration invasion. Knockdown inhibited EGF-induced EGFR activation degradation, by decreasing This study demonstrates down-regulation sufficient to suppress malignant phenotype cells signaling....
Skeletal muscle development requires the cell–cell fusion of differentiated myoblasts to form fibers. The actin cytoskeleton is known be main driving force for myoblast fusion; however, how organized direct intercellular remains unclear. Here we show that an actin- and dynamin-2–enriched protrusive structure, invadosome, required process myogenesis. Upon differentiation, acquire ability invadosomes through isoform switching a critical invadosome scaffold protein, Tks5. Tks5 directly...
Abstract Large intergenic noncoding RNA regulator of reprogramming (Linc‐RoR) was first identified as a to increase the emergence induced pluripotent stem cells through differentiated and is abnormal expression in variety malignant tumors. However, function Linc‐RoR pancreatic cancer progression needs further clarification. The data from this study demonstrated that knockdown suppressed cell proliferative capacity colony formation, while overexpression promoted these behaviors. In...
Mitochondria are dynamic organelles regulated by fission and fusion processes. The of membranes requires elaborative coordination proteins lipids is particularly crucial for the function quality control mitochondria. Phosphatidic acid (PA) on mitochondrial outer membrane generated PLD6 facilitates However, how PA promotes remains unclear. Here, we show that a protein, NME3, required PLD6-induced tethering or clustering. NME3 enriched at contact interface two closely positioned mitochondria...
Dynamin (Dyn) is a multidomain and multifunctional GTPase best known for its essential role in clathrin-mediated endocytosis (CME). Dyn2 mutations have been linked to two human diseases, centronuclear myopathy (CNM) Charcot-Marie-Tooth (CMT) disease. Paradoxically, although ubiquitously expressed embryonic development, the disease-associated mutants are autosomal dominant, but result slowly progressing tissue-specific diseases. Thus, cellular defects that cause disease remain unclear, they...
Internalization of macromolecules and membrane into cells through endocytosis is critical for cellular growth, signaling plasma (PM) tension homeostasis. Although responsive to both biochemical physical stimuli, how cues modulate endocytic pathways less understood. Contrary the accumulating discoveries on effects increased PM endocytosis, known about a decrease impacts trafficking. Here, we reveal that an acute results in phosphatidic acid (PA) production, F-actin phosphatidylinositol...
Significance Structural remodeling of neurons after insults to the nervous system includes retraction dysfunctional synaptic branches and growth primary neurites or new collateral branches. We find that genetic disruption neuronal microtubules in Caenorhabditis elegans triggered structural through RHGF-1/RhoGEF, which is normally associated with inhibited by microtubules. A conserved dual leucine zipper kinase, DLK-1, was activated RHGF-1-dependent signaling, DLK-1 transported from distal...
The mammalian target of rapamycin (mTOR) is an evolutionarily conserved kinase which assembles a signaling network that integrates diverse biochemical and mechanical cues to coordinate cell growth proliferation. Mechanical load has been well appreciated induce mTOR activation leads skeletal muscle through phospholipase D (PLD) activity phosphatidic acid (PA) production. While PA produced by PLD1 critical for upon mitogenic stimulation at the lysosome, it unclear where in muscle. Here we...
Insulin-stimulated translocation of glucose transporter 4 (GLUT4) to plasma membrane skeletal muscle is critical for postprandial uptake; however, whether the internalization GLUT4 also regulated by insulin signaling remains unclear. Here, we discover that activity dynamin-2 (Dyn2) in catalyzing endocytosis negatively cells. Mechanistically, fission Dyn2 inhibited binding with SH3 domain Bin1. In absence insulin, GSK3α phosphorylates relieve inhibition Bin1 and promotes endocytosis....
Abstract Background and Hypothesis Schizophrenia presents significant treatment challenges, particularly due to medication resistance observed in some patients receiving antipsychotics. Emerging research suggests a potential link between impaired reinforcement learning, the severity of psychotic symptoms, dopamine system abnormalities. Exploring learning therapeutic settings could provide critical insights into efficacy antipsychotic treatments. This study aimed investigate whether...
<title>Abstract</title> Plasma membrane remodeling processes are tightly regulated by the spatiotemporal distribution and dynamic conversion of phosphoinositidyl lipids (PIPs). This regulation is controlled recruitment proteins such as sorting nexin 9 (SNX9), a key mediator late-stage endocytosis macropinocytosis. Using live cell imaging, <italic>in vitro</italic> reconstitution assays, molecular dynamics simulations, we investigated how SNX9 distinguishes between PI(3,4)P<sub>2</sub>...
// Ting-Yun Chuang 1 , An-Jie Cheng I-Ting Chen Tien-Yun Lan I-Hsuan Huang Chung-Wai Shiau 2 Chia-Lin Hsu 3 Ya-Wen Liu 4 Zee-Fen Chang Ping-Hui Tseng and Jean-Cheng Kuo 1, 5, 6 Institute of Biochemistry Molecular Biology, National Yang-Ming University, Taipei 11221, Taiwan Biopharmaceutical Sciences, Microbiology Immunology, Medicine, College 10002, 5 Biophotonics & Imaging Research Center, Proteomics Correspondence to: Kuo, email: jckuo@ym.edu.tw Keywords: TNF-α secretion, dexamethasone,...