Ferroptosis, a new regulatory cell death modality, underlies the pathogenesis of broad range disorders. Although much efforts have been made to uncover molecular mechanisms, some mechanistic details ferroptosis still remain poorly understood. Particularly, functional relevance mitochondrial reactive oxygen species (ROS) in is highly controversial, which partially due fact that it remains puzzled how ROS level varies during ferroptosis. The conventional mitochondria-targeted probes may react...

We designed and synthesized an oxindole-functionalized two-photon fluorescent probe based on a naphthalimide fluorophore, which enabled in situ visualization of the overwhelming peroxynitrite flux during amyloid-β-induced neuronal ferroptosis cerebral stress live mice with Alzheimer's disease.

Abstract Skin aging is characterized by the disruption of skin homeostasis and impaired injury repair. Treatment has long been limited unclear intervention targets delivery techniques. Engineering extracellular vesicles (EVs) as an upgraded version natural EVs holds great potential in regenerative medicine. In this study, we found that expression critical antioxidant detoxification gene Gstm2 was significantly reduced skin. Thus, constructed primary fibroblasts-derived encapsulating mRNA...

MicroRNA (miRNA) plays a critical function in the progression of acute coronary syndrome (ACS) and is associated with major adverse cardiovascular events (MACEs) after undergoing percutaneous intervention (PCI). This research was designed to probe diagnostic accuracy miR-483-5p patients ACS its predictive value MACEs.118 (40 unstable angina pectoris [UAP] 78 myocardial infarction [AMI]) 75 healthy controls were enrolled. Serum detected subjects by reverse transcription-quantitative real-time...

To determine the ability of radiation to modulate kidney tubule epithelial cell expression various molecules involved in regulating extracellular matrix accumulation (collagen types I and III, fibronectin, plasminogen activator-inhibitor 1 (PAI-1), TGF-beta tissue inhibitor metalloproteinases-2 (TIMP-2)) degradation (plasminogen activators u-PA or t-PA, MMP-2 MMP-9).NRK52E rat cells were placed serum-free medium 24 h prior irradiation with single doses 1.0-10.0 Gy 137Cs gamma-rays. After...

Abstract Objective To investigate the clinical features of Han Chinese patients with anti‐neurofascin‐155 (NF155) antibody‐positive neuropathy. Methods We screened 194 peripheral neuropathy for NF155 antibodies using a cell‐based assay (CBA) and teased‐fiber immunofluorescence assay. summarized findings seropositive patients. Results The sera from 17 reacted to human embryonic kidney 293 cells transfected NF155. Eleven these had immunoglobulin G (IgG) 4 isotype, younger onset age, tremor,...

Elevated plasma level of homocysteine, also termed as hyperhomocysteinemia, is acknowledged a significant and independent risk factor Alzheimer's disease. However, the mechanistic insight has not been thoroughly elucidated yet. In this work, 3,5-dihydroxybenzyloxy was explored unique reaction trigger integrated into naphthalimide fluorophore via carbamate linker to afford new probe for •OH imaging. treatment induced aromatic hydroxylation subsequent elimination release caged fluorophore,...

Abstract Background Combined methylmalonic acidemia (MMA) and hyperhomocysteinemia, cobalamin C (cblC) type, also named cblC deficiency is a rare autosomal recessive genetic metabolic disease. It progressively causes neurological, hematologic, renal other system dysfunction. The clinical manifestations are relatively different due to the onset time of Case presentation This report describes case 26 year old man with who developed life-threatening aortic dissection acute kidney injury (AKI)...

Objective The aim of this study was to examine the role intracoronary anisodamine and diltiazem administration performed before stenting on immediate angiographic clinical outcome in patients with ST-elevation myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention (PCI). Methods STEMI during PCI were randomized two bolus injections (1 mg/5 ml) (2 (COM group, n=54) or saline (5 (diltiazem stenting. endpoint incidence no/slow reflow [thrombolysis (TIMI) flow...

The ongoing epidemic of obesity and associated illnesses highlights the need to understand biological processes that regulate energy balance. Here, we identified an important role for a protein complex called BBSome in control hepatic function. We show liver is necessary maintain body weight blood glucose levels due its requirements generate detect insulin, hormone essential metabolic regulation.

The cssR gene (ncgl1578) of Corynebacterium glutamicum encodes a repressor the TetR (tetracycline regulator) family. Its role in stress response to antibiotics/heavy metals has been investigated, but how CssR functions phenolic compounds C. rarely studied. In this study, we applied transcriptomic analysis, β-galactosidase qRT-PCR, and EMSAs analyze target genes compounds. Consistent with upregulation involved degradation compounds, ΔcssR mutant was more resistant various than wild-type...

The current study show how genetic manipulation of fat cells impacts various functions the body including sensitivity to hormone insulin.

Extracellular vesicles (EVs) are lipid-bilayer membrane-enclosed well known to mediate cell-cell communication maintain normal physiological functions. The diverse cargo of EVs encompassing proteins, lipids and miRNAs represent potential biomarkers therapeutic targets. However, the role adipocyte-derived (AdEVs) in obesity is still unknown. To address this, we performed proteomic profiling AdEVs isolated from C57BL/6 male mice that were fed either a chow diet (NCD) or high-fat high-sucrose...

Hibifolin, the highest-content bioactive flavonoid of flowers Abelmoschus manihot, was incubated with human intestinal bacteria, and four metabolites (1–4) were obtained from solution by chromatographic methods. The structures elucidated as gossypetin 8-O-β-D-4′′-deoxy-Δ 4′′-glucuropyranoside (1), (2), quercetin (3), 8-methoxy-quercetin (4), respectively, on basis UV, NMR, MS data. Metabolite 1 a new compound specific β-D-4′′-deoxy-Δ 4′′-glucuropyranosyl moiety, which formed through unique...

The interplay of the enteric nervous system (ENS) and SIP syncytium (smooth muscle cells–interstitial cells Cajal–PDGFRα+ cells) plays an important role in regulation gastrointestinal (GI) motility. This study aimed to investigate dynamic regulatory mechanisms ENS-SIP on colon motility during postnatal development. Colonic samples 1-week-old (PW1), 3-week-old (PW3), 5-week-old (PW5) mice were characterized by RNA sequencing, qPCR, Western blotting, isometric force recordings (IFR), colonic...

The BBSome, a protein complex of 8 Bardet-Biedl syndrome (BBS) proteins including BBS1, has emerged as an important regulator metabolic function. BBSome deficient human and mice display several features obesity type 2 diabetes. Here, we show that lacking the specifically in liver which were generated by crossing Bbs1 fl/fl with liver-specific (albumin) Alb Cre exhibit significantly higher lipid droplets liver, Oil Red-O staining, primary cultured hepatocytes (5.7%±0.4% vs 2.4%±0.6%,...

Best known as the powerhouse of cell, mitochondria play a crucial role in obesity and associated cardiovascular diseases. A-kinase anchoring protein 1 (AKAP1) is mitochondrial scaffold that regulates function by promoting kinase A (PKA)-mediated phosphorylation Dynamin-related at Ser637 through recruitment PKA to outer membrane. However, AKAP1 regulation blood pressure development hypertension not known. Here, we used mice harboring Akap1 gene deletion investigate importance obesity-...

Increasing evidence suggests that tumor cells exhibit extreme plasticity in migration modes order to adapt microenvironments. However, the underlying mechanism for governing mode switching is still unclear. Here, we revealed epithelial could develop a stable directional driven by hyperactivated ERK activity. This highly activated and dynamically changing activity, called pERK transition, crucial inducing switch from pauses state movement also necessary maintaining mode. PERK transition...

Primary cilia possess an array of receptors and signaling from this organelle is critical for neuronal function. BBS1 important component a receptor trafficking complex (BBSome) that facilitates in both primary plasma membrane. Humans harboring mutations develop obesity type 2 diabetes. Recent studies our lab others have shown deletion hypothalamic neurons lead to glucose dysregulation. However, the role hindbrain metabolic control unknown. We hypothesized brainstem regulates metabolism...