A5051840811- Cardiac Fibrosis and Remodeling
- Ion channel regulation and function
- Cardiac Valve Diseases and Treatments
- Trace Elements in Health
- Signaling Pathways in Disease
- MicroRNA in disease regulation
- Cardiac electrophysiology and arrhythmias
- Nanopore and Nanochannel Transport Studies
- Advanced biosensing and bioanalysis techniques
- Ion Channels and Receptors
- Circular RNAs in diseases
- Neuroscience and Neural Engineering
- Electrochemical Analysis and Applications
- Viral Infections and Immunology Research
- Extracellular vesicles in disease
- Muscle Physiology and Disorders
- Cardiovascular Disease and Adiposity
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cancer-related molecular mechanisms research
- Calcium signaling and nucleotide metabolism
- Peptidase Inhibition and Analysis
- Photoreceptor and optogenetics research
- NF-κB Signaling Pathways
Imperial College London
2020-2025
Lung Institute
2023
University of St Andrews
2016-2017
Abstract There is an unmet need to develop low-cost, rapid and highly multiplexed diagnostic technology platforms for quantitatively detecting blood biomarkers advance clinical diagnostics beyond the single biomarker model. Here we perform nanopore sequencing of DNA-barcoded molecular probes engineered recognize a panel analytes. This allows simultaneous quantitative detection at least 40 targets, such as microRNAs, proteins neurotransmitters, on basis translocation dynamics each probe it...
Cardiac fibrosis occurs in a wide range of cardiac diseases and is characterised by the transdifferentiation fibroblasts into myofibroblasts these cells produce large quantities extracellular matrix, resulting myocardial scar. The profibrotic process multi-factorial, meaning identification effective treatments has been limited. antifibrotic effect bile acid ursodeoxycholic (UDCA) established cases liver however its mechanism role less well understood.
Abstract We present a novel framework, Opto-SICM, for studies of cellular interactions in live cells with high spatiotemporal resolution. The approach combines scanning ion conductance microscopy, SICM, and cell-type-specific optogenetic interrogation. Light-excitable cardiac fibroblasts (FB) myofibroblasts (myoFB) were plated together non-modified cardiomyocytes (CM) then paced periodic illumination. Opto-SICM reveals the extent FB/myoFB-CM cell-cell contacts dynamic changes over time not...
Abstract Background Cardiac fibrosis can be triggered by several pathologies, including ischemic heart disease and aortic stenosis (AS). is brought about uncontrolled extracellular matrix (ECM) deposition myofibroblasts. Interleukin-11 (IL-11) has been firmly demonstrated to a major trigger of multi-organ fibrosis. However, the molecular mechanisms underpinning IL-11-induced requires further characterisation. Recent studies indicate that microRNA (miRNA) dysregulation contributes...
Abstract Cardiac fibrosis occurs in a wide range of cardiac diseases and is characterised by the transdifferentiation fibroblasts (FB) into myofibroblasts (MFB). Myofibroblasts produce large quantities extracellular matrix proteins, resulting myocardial scar. The antifibrotic effect bile acid ursodeoxycholic (UDCA) established cases liver but not adult myocardium. Our hypothesis is: UDCA heart, mediated membrane receptor Takeda G protein-coupled 5 (TGR5). We constructed predictive network...
Abstract Currently, most blood tests in a clinical setting only investigate handful of markers. A low-cost, rapid, and highly multiplexed platform for the quantitative detection biomarkers has potential to advance diagnostics beyond single biomarker paradigm. In this study, we perform nanopore sequencing barcoded molecular probes that have been engineered recognise panel biological targets (miRNAs, proteins, small molecules such as neurotransmitters), allowing simultaneous detection. Our...
Abstract Background Cardiac fibrosis is associated with inflammation and extracellular matrix (ECM) accumulation. A pro-fibrotic cytokine, IL11 induces cardiac fibroblasts conversion to myofibroblasts expressing α-smooth muscle actin (α-SMA) ECM. MicroRNAs (miRNAs) are a class of small non-coding RNAs which participate in regulation gene expression; Although mainly intracellular, miRNAs can be released into the blood stream where they readily detected. Purpose To screen upregulated following...