A5028452422- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- MicroRNA in disease regulation
- Renal cell carcinoma treatment
- SARS-CoV-2 and COVID-19 Research
- Renal and related cancers
- Cancer Genomics and Diagnostics
- Virus-based gene therapy research
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- RNA modifications and cancer
- Molecular Biology Techniques and Applications
- Plant Virus Research Studies
- Viral Infections and Vectors
- RNA Interference and Gene Delivery
- Respiratory viral infections research
- Breast Cancer Treatment Studies
- Cancer Cells and Metastasis
- Animal Disease Management and Epidemiology
- Breast Lesions and Carcinomas
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Adolescent and Pediatric Healthcare
- Multiple Myeloma Research and Treatments
Onkologikoa
2024-2025
Biogipuzkoa Health Research Institute
2012-2023
The Pirbright Institute
2007-2022
Molecular Oncology (United States)
2017-2018
Compton Community College District
2012
Newbury College
2009-2011
Organic Research Centre
2007
UK Centre for Ecology & Hydrology
2004
Institute of Virology of the Slovak Academy of Sciences
2002
University of Oxford
2002
A strain of Tick-borne encephalitis virus designated Zausaev (Za) was isolated in Siberia from a patient who died progressive (2-year) form tick-borne 10 years after being bitten by tick. The complete genomic sequence this determined, and an attempt made to correlate the with biological characteristics virus. Phylogenetic analysis demonstrated that belongs Siberian subtype Comparison Za two related viruses, Far Eastern isolate, Sofjin, Vasilchenko, revealed differences among three viruses...
We have previously demonstrated that the replacement of S gene from an avirulent strain (Beaudette) infectious bronchitis virus (IBV) with a virulent (M41) resulted in recombinant (BeauR-M41(S)) vitro cell tropism but was still avirulent. In order to investigate whether any other structural or accessory genes played role pathogenicity we now replaced these Beaudette those M41. The IBV effect chimaeric replicase derived and rest genome This it is possible exchange large region genome,...
Millions of domestic and wild European rabbits (Oryctolagus cuniculus) have died in Europe, Asia, Australia New Zealand during the past 17 years following infection by Rabbit haemorrhagic disease virus (RHDV). This highly contagious deadly was first identified China 1984. Epidemics RHDV then radiated across Europe until apparently appeared Britain 1992. However, this concept radiation a new virulent from is not entirely consistent with serological molecular evidence. study shows, using...
We have shown previously that replacement of the spike (S) gene apathogenic IBV strain Beau-R with from pathogenic same serotype, M41, resulted in an virus, BeauR-M41(S), conferred protection against challenge M41 [1]. constructed a recombinant IBV, BeauR-4/91(S), genetic backbone but expressing protein 4/91(UK), which belongs to different serogroup as Beaudette or M41. Similar our previous findings clinical signs observations showed S 4/91 virus did not confer pathogenicity rIBV...
The spike (S) glycoprotein of the avian gammacoronavirus infectious bronchitis virus (IBV) is comprised two subunits (S1 and S2), has a role in virulence vivo, responsible for cellular tropism vitro We have previously demonstrated that replacement S ectodomain from avirulent Beaudette strain IBV with corresponding region virulent M41-CK resulted recombinant virus, BeauR-M41(S), cell M41-CK. able to replicate both primary chick kidney cells Vero cells, whereas replicates only. In order...
Coronavirus subgenomic mRNA (sgmRNA) synthesis occurs via a process of discontinuous transcription involving regulatory sequences (TRSs) located in the 5' leader sequence (TRS-L) and upstream each structural group-specific gene (TRS-B). Several gammacoronaviruses including infectious bronchitis virus (IBV) contain putative open reading frame (ORF), localized between M 5, which is controversial due to perceived absence TRS. We have studied novel sgmRNA associated with this potential ORF found...
The circulating transcriptome is a valuable source of cancer biomarkers, which, with the exception microRNAs (miRNAs), remains relatively unexplored. To elucidate which RNAs are present in plasma from melanoma patients and could be used to distinguish healthy individuals, we next generation sequencing (NGS), validation was carried out by qPCR and/or ddPCR. We identified 442 different samples, eleven were differentially expressed (p < 0.05). Levels miR-134-5p miR-320a-3p significantly...
The genus Flavivirus consists of more than 70 virus species and subtypes, the majority which are transmitted by mosquitoes or ticks, although some have no known vector (NKV). ability these viruses to infect cultured cells derived from mosquito tick offers a useful insight into suitability such vectors harbour replicate particular viruses. We undertook comparative study susceptibility mammalian Vero cells, clonal cell line (C6/36) recently developed lines ticks (Acari: Ixodidae) Ixodes...
Chronic lymphocytic leukemia (CLL) is an incurable disease accounting for almost one‐third of leukemias in the Western world. Aberrant expression microRNAs (miRNAs) a well‐established characteristic CLL, and robust nature miRNAs makes them eminently suitable liquid biopsy biomarkers. Using nested case–control study within European Prospective Investigation into Cancer Nutrition (EPIC), predictive values five promising human ( hsa‐miR‐16‐5p , hsa‐miR‐29a‐3p hsa‐miR‐150‐5p hsa‐miR‐155‐5p...
The Gammacoronavirus infectious bronchitis virus (IBV) is the etiological agent of bronchitis, an acute, highly contagious, economically important disease poultry. Vaccination achieved using a mixture live attenuated vaccines for young chicks and inactivated as boosters laying hens.
Infectious bronchitis virus (IBV) causes an infectious respiratory disease of domestic fowl that affects poultry all ages causing economic problems for the industry worldwide. Although IBV is controlled using live attenuated and inactivated vaccines it continues to be a major problem due existence many serotypes, determined by surface spike protein resulting in poor cross-protection, loss immunogenicity associated with vaccine production. Live are produced repeated passage embryonated eggs...
Despite advances in the management of advanced clear cell renal carcinoma (ccRCC), robust biomarkers for prognosis and therapeutic response prediction remain elusive. Dysregulation intrarenal renin–angiotensin system (RAS) has been implicated carcinogenesis but little explored, particularly regarding biomarker discovery innovation. Consequently, this study investigates immunohistochemical expression clinical relevance Mas-related G-protein-coupled receptor D (MrgD) patients with ccRCC who...
Bortezomib action in multiple myeloma: microRNA-mediated synergy (and miR-27a /CDK5 driven sensitivity)?
Early diagnosis of laryngeal squamous cell carcinoma (LSCC) at the stage dysplasia could greatly improve outcome affected patients. For first time we compared mutational landscape non-progressing (NPD; n = 42) with progressing (PD; 24), along patient-matched LSCC biopsies; a total 90 samples. Using targeted next-generation sequencing identified non-synonymous mutations in six genes (PIK3CA, FGFR3, TP53, JAK3, MET, FBXW7), and were validated by Sanger and/or qPCR. Analysis was extended silico...
Prostate cancer (PCa) is the second most common of men and typically slow-growing asymptomatic. The use blood PSA as a screening method has greatly improved PCa diagnosis, but high levels false positives raised much interest in alternative biomarkers. We used next-generation sequencing (NGS) to elucidate urinary transcriptome whole urine collected from high-stage low-stage patients well with confounding diagnosis benign hyperplasia (BPH). identified validated five differentially expressed...
Even with the widespread uptake of vaccines, SARS-CoV-2-induced COVID-19 pandemic continues to overwhelm many healthcare systems worldwide. Consequently, massive scale molecular diagnostic testing remains a key strategy control ongoing pandemic, and need for instrument-free, economic easy-to-use alternatives PCR goal providers, including WHO. We developed test (Repvit) based on gold nanoparticles that can detect SARS-CoV-2 RNA directly from nasopharyngeal swab or saliva samples limit...
The replicase gene of the coronavirus infectious bronchitis virus (IBV) encodes 15 non-structural proteins (nsps). Nsp 3 is a multi-functional protein containing conserved ADP-ribose-1″-phosphatase (ADRP) domain. crystal structures domain from two strains IBV, M41 (virulent) and Beaudette (avirulent), identified key difference; contains triple-glycine motif, whilst glycine-to-serine mutation that predicted to abolish ADRP activity. Although activity has not been formally demonstrated for IBV...
Papillary renal neoplasm with reversed polarity (PRNRP) is a recently described rare neoplasm. Traditionally, it was considered variant of papillary cell carcinoma (PRCC). However, several studies reported significant differences between PRNRP and PRCC in terms clinical, morphological, immunohistochemical molecular features. Nonetheless, remains poorly understood entity. We used microarray analysis to elucidate the non-coding RNA (ncRNA) gene expression profiles 10 cases compared them other...
Sunitinib has greatly improved the survival of clear cell renal carcinoma (ccRCC) patients in recent years. However, 20–30% treated do not respond. To identify miRNAs and genes associated with a response, comparisons were made between biopsies from responder non-responder ccRCC patients. Using integrated transcriptomic analyses, we identified 37 60 respective target genes, which significantly NF-kappa B, PI3K-Akt MAPK pathways. We validated expression (miR-223, miR-155, miR-200b, miR-130b)...
(1) Objective: To develop a clinically useful nomogram that may provide more individualized and accurate estimation of cancer-specific survival (CSS) for patients with clear-cell (CC) metastatic renal cell carcinoma (mRCC) treated nephrectomy tyrosine kinase Inhibitor (TKI) based sequential therapy; (2) Methods: A prospectively maintained database 145 mRCC between 2008-2018 was analyzed to predict CSS receiving sunitinib 2nd 3rd line therapies according current standards practice. taking...
(1) Background & Aims: The roles of different cells in the tumor microenvironment (TME) are critical to metastatic process. phenotypic transformation liver is one most important stages hepatic metastasis progression colorectal cancer (CRC). Our aim was identify major molecules (i.e., genes, miRNAs and proteins) involved this (2) Methods: We isolated performed whole-genome analysis gene, miRNA, protein expression three types (Ito cells, Kupffer sinusoidal endothelial cells) from TME a...