A5103200383- Amino Acid Enzymes and Metabolism
- Enzyme Structure and Function
- Gut microbiota and health
- RNA and protein synthesis mechanisms
- Machine Learning in Bioinformatics
- Genetic Associations and Epidemiology
- Cancer Research and Treatments
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Cell death mechanisms and regulation
- Polyamine Metabolism and Applications
- Molecular Biology Techniques and Applications
- Marine Sponges and Natural Products
- interferon and immune responses
- Metabolism and Genetic Disorders
- Genetics and Neurodevelopmental Disorders
- Forensic and Genetic Research
- Genomic variations and chromosomal abnormalities
- Transgenic Plants and Applications
- Bacterial Genetics and Biotechnology
- Genetic Mapping and Diversity in Plants and Animals
- Biopolymer Synthesis and Applications
- Insurance, Mortality, Demography, Risk Management
- Genetic Syndromes and Imprinting
- Animal Genetics and Reproduction
Morehouse School of Medicine
2013-2016
AID Atlanta
2015
Georgia State University
2008-2011
Abstract Background With the improvement of bacterial detection, theory sterile female upper reproductive tract has been frequently challenged in recent years. However, thus far, no researchers have used ovaries as study targets. Methods Six women who were diagnosed with ovarian cancer included group, and ten a noncancerous condition (including three patients uterine myoma seven adenomyosis) control group. Immunohistochemistry staining using an antibacterial lipopolysaccharide (LPS) antibody...
DADH catalyzes the flavin-dependent oxidative deamination of d-amino acids to corresponding α-keto and ammonia. Here we report first X-ray crystal structures at 1.06 Å resolution its complexes with iminoarginine (DADHred/iminoarginine) iminohistidine (DADHred/iminohistidine) 1.30 resolution. The structure comprises an unliganded conformation a product-bound conformation, which is almost identical DADHred/iminoarginine structure. active site was partially occupied product (30% occupancy) that...
D-arginine dehydrogenase from Pseudomonas aeruginosa catalyzes the oxidation of to iminoarginine, which is hydrolyzed in solution ketoarginine and ammonia. In present study, we have genetically engineered an untagged form enzyme that was purified high levels characterized its kinetic properties. The a true does not react with molecular oxygen. Steady-state studies or D-histidine as substrate PMS electron acceptor established ping-pong bi-bi mechanism. With fast dead-end complex reduced...
Population stratification is a growing concern in genetic-association studies. Averaged ancestry at the genome level (global ancestry) insufficient for detecting population substructures and correcting stratifications association Local phase are needed human genetic studies, but current technologies cannot be applied on entire data due to various technical caveats. Here we developed novel approach (aMAP, of Modern Admixed Populations) inferring local phased ancestry. It took about 3 seconds...
Background The use of DNA from archival formalin and paraffin embedded (FFPE) tissue for genetic epigenetic analyses may be problematic, since the is often degraded only limited amounts available. Thus, it currently not known whether genome-wide methylation can reliably assessed in FFPE tissue. Methodology/Principal Findings Ovarian tissues, which were obtained formalin-fixed paraffin-embedded either 1999 or 2011, sectioned stained with hematoxylin-eosin (H&E).Epithelial cells captured by...
Epigenetic heritability is an important issue in the field of genetics and also development many human diseases. In this study, we created a transgenic rat model investigated transgenerational methylation patterns these animals. The transgene DNA fragment was unmethylated before it injected into pronucleus, so good to study inheritance patterns. We performed bisulfite sequencing on 23 CpG dinucleotides across three generations two tissues. observed that heavily methylated liver (87.53%) from...
d-Arginine dehydrogenase (DADH) catalyzes the flavin-dependent oxidative deamination of d-arginine and other d-amino acids to corresponding imino acids. The 1.07 Å atomic-resolution structure DADH crystallized with d-leucine unexpectedly revealed a covalent N(5) flavin adduct, instead expected iminoleucine product in active site. This acyl adduct has been successfully reproduced by photoreduction presence 4-methyl-2-oxopentanoic acid (ketoleucine). may be released readily because weak...
Endometrial cancer is the most common malignancy of female reproductive system. The three spread patterns endometrial are local invasion, lymphatic and hematogenous spread. Vaginal metastasis occurs by submucosal or vascular metastases in ~10% patients with clinical stage I disease. implantation extremely rare. Here we present a case carcinoma (International Federation Gynecology Obstetrics IA) to vagina as opposed any methods mentioned above. This conclusion was confirmed mainly from...
Abstract Summary: We present a software tool called GenomeLaser that determines the haplotypes of each person from unphased high-throughput genotypes in family pedigrees. This method features high accuracy, chromosome-range phasing distance, linear computing, flexible pedigree types and genetic marker types. Availability implementation: http://www.4dgenome.com/software/genomelaser.html. Contact: qsong@msm.edu Supplementary information: data are available at Bioinformatics online.
Abstract The female upper reproductive tract, including the uterus, fallopian tubes and ovaries, is believed to be a sterile environment. With improvement of bacterial detection, theory tract has been frequently challenged in recent years. However, thus far, no researchers have used ovaries as study targets. Six women who were diagnosed with ovarian cancer included group, ten noncancerous condition (including three patients uterine myoma seven adenomyosis) control group. Immunohistochemistry...
The theory of a "sterile" female upper reproductive tract has been encountering increasing opposition due to advancements in bacterial detection. However, whether ovaries contain bacteria not yet confirmed yet. Herein, an experiment detect ovarian tissues was introduced. We chose cancer patients the group and noncancerous control group. 16S rRNA gene sequencing used differentiate from groups. Furthermore, we predicted functional composition identified by using BugBase PICRUSt. This method...
The theory of a "sterile" female upper reproductive tract has been encountering increasing opposition due to advancements in bacterial detection. However, whether ovaries contain bacteria not yet confirmed yet. Herein, an experiment detect ovarian tissues was introduced. We chose cancer patients the group and noncancerous control group. 16S rRNA gene sequencing used differentiate from groups. Furthermore, we predicted functional composition identified by using BugBase PICRUSt. This method...