A5028548504- Algal biology and biofuel production
- Photosynthetic Processes and Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Light effects on plants
- Enzyme Catalysis and Immobilization
- Diatoms and Algae Research
- Antioxidant Activity and Oxidative Stress
- Biofuel production and bioconversion
- Marine and coastal ecosystems
- Protist diversity and phylogeny
- Bacterial Genetics and Biotechnology
- Photoreceptor and optogenetics research
- Microbial Community Ecology and Physiology
- Circadian rhythm and melatonin
- Magnetic and Electromagnetic Effects
- Lipid metabolism and biosynthesis
- Protein purification and stability
- Biocrusts and Microbial Ecology
- Metabolomics and Mass Spectrometry Studies
- Microbial metabolism and enzyme function
- Microbial Fuel Cells and Bioremediation
- Nanoparticle-Based Drug Delivery
- Genomics and Phylogenetic Studies
- Seed Germination and Physiology
- Science, Research, and Medicine
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Centre National de la Recherche Scientifique
2016-2025
Université de Toulouse
2018-2025
Institut National des Sciences Appliquées de Toulouse
2022-2024
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2016-2024
Systèmes Membranaires, Photobiologie, Stress et Détoxication
2011-2023
Biotechnology Institute
2022
Colorado State University
2016-2022
Université Toulouse III - Paul Sabatier
2018
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2013-2016
Abstract Recently developed transgenic techniques to explore and exploit the metabolic potential of microalgae present several drawbacks associated with delivery exogenous DNA into cells its subsequent integration at random sites within genome. Here, we report a highly efficient multiplex genome-editing method in diatom Phaeodactylum tricornutum , relying on biolistic CRISPR-Cas9 ribonucleoproteins coupled identification two endogenous counter-selectable markers, PtUMPS PtAPT . First,...
Abstract The orange carotenoid protein (OCP) serves as a sensor of light intensity and an effector phycobilisome (PB)–associated photoprotection in cyanobacteria. Structurally, the OCP is composed two distinct domains spanned by single chromophore. Functionally, response to high light, converts from dark-stable form, OCPO, active red OCPR. C-terminal domain has been implicated dynamic plays role switching off OCP's photoprotective through its interaction with fluorescence recovery protein....
Significance Protection from overexcitation is one of the most important requirements all photosynthetic organisms. Here we present a model based on coupled cross-linking/mass spectrometry and site-directed mutagenesis means by which orange carotenoid protein (OCP) binds to phycobilisome (PBS) antenna complex avoid photodamage. The shows that must actively burrow into complex, separating PBS rings in process. This penetration explains for first time, our knowledge, how OCP could approach...
Summary Photoacclimation consists of short‐ and long‐term strategies used by photosynthetic organisms to adapt dynamic light environments. Observable photophysiology changes resulting from these have been in coarse‐grained models predict light‐dependent growth rates. However, the contribution broader metabolic network, relevant species‐specific fitness, is not accounted for simple models. We incorporated experimental data with genome‐scale modeling characterize organism‐level, model diatom...
Photosynthetic microbes respond to changing light environments balance photosynthetic process with induced damage and photoinhibition. There have been very few characterizations of physiology or biomass partitioning during the day in mass culture. Understanding constraints on efficiency accumulation are necessary for engineering superior strains cultivation methods. We observed nutrient replete Phaeodactylum tricornutum growing that mimic those found rapidly mixing, outdoor, low...
Summary Photoautotrophic growth in nature requires the accumulation of energy‐containing molecules via photosynthesis during daylight to fuel nighttime catabolism. Many diatoms store photosynthate as neutral lipid triacylglycerol (TAG). While pathways diatom fatty acid and TAG synthesis appear be well conserved with plants, catabolism downstream β‐oxidation have not been characterised diatoms. We identified a putative mitochondria‐targeted, bacterial‐type acyl‐CoA dehydrogenase (PtMACAD1)...
Abstract Cyanobacteria have developed a photoprotective mechanism that decreases the energy arriving at reaction centers by increasing thermal dissipation level of phycobilisome (PB), extramembranous light-harvesting antenna. This is triggered photoactive Orange Carotenoid Protein (OCP), which acts both as photosensor and quencher. The OCP binds core PB. structure this differs in diverse cyanobacterial strains. Here, using two isolated OCPs four classes PBs, we demonstrated differences exist...
Abstract Diatoms (Bacillariophyceae) accumulate neutral storage lipids in lipid droplets during stress conditions, which can be rapidly degraded and recycled when optimal conditions resume. Since nutrient light availability fluctuate marine environments, turnover is essential for diatom dominance of ecosystems. have garnered attention their potential to provide a sustainable source omega-3 fatty acids. Several independent proteomic studies isolated from the model oleaginous pennate...
Bacterial microcompartments (BMCs) are self-assembling protein megacomplexes that encapsulate metabolic pathways. Although approximately 20% of sequenced bacterial genomes contain operons encoding putative BMCs, few have been thoroughly characterized, nor any in the most studied
Phasing biological and physiological processes to periodic light-dark cycles is crucial for the life of most organisms. Marine diatoms, as many phytoplanktonic species, exhibit rhythms, yet their molecular timekeepers remain largely uncharacterized. Recently, bHLH-PAS protein RITMO1 has been proposed act a regulator diatom circadian rhythms. In this study, we first determined conditions monitor clock activity its perturbation in model species Phaeodactylum tricornutum by using cell...
SUMMARY Characterizing photosynthetic productivity is necessary to understand the ecological contributions and biotechnology potential of plants, algae, cyanobacteria. Light capture efficiency photophysiology have long been characterized by measurements chlorophyll fluorescence dynamics. However, these investigations typically do not consider metabolic network downstream light harvesting. By contrast, genome‐scale models species‐specific capabilities but yet incorporate rapid regulation...
Abstract Bacterial microcompartments (BMCs) are self-assembling protein megacomplexes that encapsulate metabolic pathways. Although approximately 20% of sequenced bacterial genomes contain operons encoding putative BMCs, few have been thoroughly characterized, nor any in the most studied Escherichia coli strains. We used an interdisciplinary approach to gain deep molecular and functional insights into ethanolamine utilization (Eut) BMC system encoded by eut operon E. K-12. The genotype was...
Summary Phasing biological and physiological processes to periodic light-dark cycles is crucial for the life of most organisms. Marine diatoms, as many phytoplanktonic species, exhibit rhythms, yet their molecular timekeepers remain largely uncharacterized. Recently, bHLH-PAS protein RITMO1 has been proposed act a regulator circadian rhythms. In this study, we first determined conditions monitor clock activity its perturbation in diatom model species Phaeodactylum tricornutum by using cell...
Abstract Diatoms, an evolutionarily successful group of microalgae, display high levels intraspecific variability in natural populations. However, the process generating such diversity is unknown. Here we estimated within a diatom population and subsequently mapped genomic changes arising cultures clonally propagated from single cells. We demonstrate that genome rearrangements mitotic recombination between homologous chromosomes underlie clonal variability, resulting haplotype accompanied by...