A5019978092- Plant-Microbe Interactions and Immunity
- Plant Parasitism and Resistance
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Seed Germination and Physiology
- Plant tissue culture and regeneration
- Polysaccharides and Plant Cell Walls
- Plant responses to water stress
- Plant Molecular Biology Research
- Plant pathogens and resistance mechanisms
- Plant Reproductive Biology
- Plant Pathogenic Bacteria Studies
- Photosynthetic Processes and Mechanisms
- Powdery Mildew Fungal Diseases
- Plant Surface Properties and Treatments
- Leaf Properties and Growth Measurement
- Flowering Plant Growth and Cultivation
- Herbal Medicine Research Studies
- Calcium signaling and nucleotide metabolism
- Plant Genetic and Mutation Studies
- Clostridium difficile and Clostridium perfringens research
- Historical Geography and Cartography
- Plant Disease Resistance and Genetics
- Machine Learning in Bioinformatics
Sainsbury Laboratory
2013-2018
Norwich Research Park
2013-2018
AgroParisTech
2008-2011
Institut Jean-Pierre Bourgin
2008-2011
Max Planck Society
2010
Max Planck Institute for Plant Breeding Research
2010
Département Santé des Plantes et Environnement
2006
Université de Rennes
2004
Ecosystèmes, Biodiversité, Evolution
2004
Abstract Reactive oxygen species (ROS) are potent signal molecules rapidly generated in response to stress. Detection of pathogen-associated molecular patterns induces a transient apoplastic ROS through the function NADPH respiratory burst oxidase homologs D (RbohD). However, little is known about regulation pattern-elicited or its role plant immunity. We investigated production triggered by bacterial flagellin (flg22) Arabidopsis (Arabidopsis thaliana). The oxidative was diminished...
Plasma membrane compartmentalization spatiotemporally regulates cell-autonomous immune signaling in animal cells. To elucidate immediate early protein dynamics at the plant plasma response to bacterial pathogen-associated molecular pattern (PAMP) flagellin (flg22) we employed quantitative mass spectrometric analysis on detergent-resistant membranes (DRMs) of Arabidopsis thaliana suspension This approach revealed rapid and profound changes DRM composition following PAMP treatment, prominently...
Sensing of potential pathogenic bacteria is critical importance for immunity. In plants, this involves plasma membrane-resident pattern recognition receptors, one which the FLAGELLIN SENSING 2 (FLS2) receptor kinase. Ligand-activated FLS2 receptors are internalized into endosomes. However, extent to these spatiotemporal dynamics generally present among (PRRs) and their regulation remain elusive. Using live-cell imaging, we show that at least three other kinases associated with plant...
Cysteine-rich receptor-like kinases (CRKs) are transmembrane proteins characterized by the presence of two domains unknown function 26 (DUF26) in their ectodomain. The CRKs form one largest groups protein plants, but biological functions have so far remained largely uncharacterized. We conducted a large-scale phenotyping approach nearly complete crk T-DNA insertion line collection showing that control important aspects plant development and stress adaptation response to biotic abiotic...
The formation of abnormal amino acid residues is a major source spontaneous age-related protein damage in cells. l-isoaspartyl methyltransferase (PIMT) combats misfolding resulting from by catalyzing the conversion to their normal l-aspartyl forms. In this way, PIMT repair enzyme system contributes longevity and survival bacterial animal kingdoms. Despite discovery activity plants two decades ago, role during plant stress adaptation seed remains undefined. work, we have isolated Arabidopsis...
The plant immune receptor FLAGELLIN SENSING 2 (FLS2) is present at the plasma membrane and internalized following activation of its ligand flagellin (flg22). We show that ENDOSOMAL SORTING COMPLEX REQUIRED FOR TRANSPORT (ESCRT)-I subunits play roles in FLS2 endocytosis Arabidopsis. VPS37-1 co-localizes with endosomes immunoprecipitates upon flg22 elicitation. Vps37-1 mutants are reduced flg22-induced but not labeled by Rab5 GTPases suggesting a defect trafficking rather than formation...
Summary Successful pathogens counter immunity at multiple levels, mostly through the action of effectors. Pseudomonas syringae secretes c . 30 effectors, some which have been shown to inhibit plant triggered upon perception conserved pathogen‐associated molecular patterns ( PAMP s). One these is H op M 1, impairs late immune responses targeting vesicle trafficking‐related A t MIN 7 for degradation. Here, we report that in planta expressed 1 suppresses two early ‐triggered responses,...
The Pseudomonas syringae effector AvrB targets multiple host proteins during infection, including the plant immune regulator RPM1-INTERACTING PROTEIN4 (RIN4) and RPM1-INDUCED PROTEIN KINASE (RIPK). In presence of AvrB, RIPK phosphorylates RIN4 at Thr-21, Ser-160, Thr-166, leading to activation receptor RPM1. Here, we investigated role phosphorylation in susceptible Arabidopsis thaliana genotypes. Using circular dichroism spectroscopy, show that is a disordered protein affects flexibility....
Significance Recognition of pathogen-associated molecular patterns (PAMPs) by the host immune system is essential for activation innate immunity in plants and animals. In plants, receptor kinase FLAGELLIN SENSING 2 (FLS2) recognizes flagellin, a PAMP from bacteria. Here, we found that glycosylphosphatidylinositol (GPI)-anchored protein LORELEI-LIKE GPI-ANCHORED PROTEIN 1 (LLG1) associates with FLS2 regulates plant modulating accumulation signaling. LLG1 functions as chaperone coreceptor...
Abstract We aimed to evaluate whether changes in maize (Zea mays) leaf expansion rate response environmental stimuli or developmental gradients are mediated by common specific expansins, a class of proteins known enhance cell wall extensibility. Among the 33 expansin putative genes analyzed, 19 were preferentially expressed at some point elongation zone and these expansins could be organized into three clusters related division, maximal expansion, differentiation. Further analysis spatial...
Summary Guard cells dynamically adjust their shape in order to regulate photosynthetic gas exchange, respiration rates and defend against pathogen entry. Cell changes are determined by the interplay of cell wall material properties turgor pressure. To investigate this relationship between pressure, shape, we focused on kidney‐shaped stomata developed a biomechanical model guard pair. Treating as composite pectin‐rich matrix embedded with cellulose microfibrils, show that strong,...
Summary • In plants, polyamines can generally be synthesized by the ornithine decarboxylase and arginine pathways. However, model plant Arabidopsis thaliana appears to possess only pathway. As two paralogous ARGININE DECARBOXYLASE ( ADC ) genes are present in , we investigated differential expression potential differences of promoter activity during seedling development under specific stress conditions. •P romoter activities were studied stable homozygotic transformants harbouring...
Stomata are an attractive model for studying the physiological responses of plants to various environmental stimuli 1–3 . Of morphological parameters that represent degree stomatal opening, length minor axis pore (the aperture) has been most commonly used dissect molecular basis its regulation. Measuring apertures is time consuming and labour intensive, preventing their use in large-scale studies. Here, we completely automated this process by developing a program called DeepStomata , which...
Expansion of gene families facilitates robustness and evolvability biological processes but impedes functional genetic dissection signalling pathways. To address this, quantitative analysis single cell responses can help characterize the redundancy within families. We developed high-throughput imaging stomatal closure, a response plant guard cells, performed reverse screen in group Arabidopsis mutants to five stimuli. Focussing on intersection between endomembrane system, we identified eight...
High throughput confocal imaging poses challenges in the computational image analysis of complex subcellular structures such as microtubule cytoskeleton. Here, we developed CellArchitect , an automated tool that quantifies changes to patterns illustrated by markers plants. We screened microtubule‐targeted herbicides and demonstrate high with integrated can distinguish effects induced known indaziflam trifluralin. The same platform was used examine 6 other compounds herbicidal activity, at...