A5024430695- Neonatal Respiratory Health Research
- Cystic Fibrosis Research Advances
- Inhalation and Respiratory Drug Delivery
- Advanced Drug Delivery Systems
- Tracheal and airway disorders
- Virus-based gene therapy research
- Respiratory viral infections research
- RNA Interference and Gene Delivery
- Congenital Diaphragmatic Hernia Studies
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Advancements in Transdermal Drug Delivery
- Viral gastroenteritis research and epidemiology
- Energy Harvesting in Wireless Networks
- Central Venous Catheters and Hemodialysis
- Hematopoietic Stem Cell Transplantation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Biochemical Analysis and Sensing Techniques
- Congenital Anomalies and Fetal Surgery
- Asthma and respiratory diseases
- Renal and related cancers
- Neuroscience of respiration and sleep
- Olfactory and Sensory Function Studies
- Connective tissue disorders research
- Intravenous Infusion Technology and Safety
University of Bari Aldo Moro
2021-2025
Eni (Italy)
2022
University of Foggia
2010-2019
Guglielmo da Saliceto Hospital
2015-2016
Soonchunhyang University
2016
Karolinska Institutet
2016
Ospedali Riuniti di Foggia
2008-2014
Brandeis University
2010
Cystic Fibrosis Research Foundation
2006-2008
University of Oxford
2008
Chronic respiratory diseases, whose one of the hallmarks is oxidative stress, are still incurable and need novel therapeutic tools pharmaceutical agents. The phenolic compounds contained in grape endowed with well-recognized anti-oxidant, anti-inflammatory, anti-cancer, anti-aging activities. Considering that natural anti-oxidants, such as proanthocyanidins, have poor water solubility oral bioavailability, we developed a drug delivery system based on solid lipid nanoparticles (SLN), apt to...
We have demonstrated that Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) overexpression in CFBE41o- cells induces a significant redistribution of F508del cystic fibrosis transmembrane conductance regulator (CFTR) from the cytoplasm to apical membrane and rescues CFTR-dependent chloride secretion. Here, we observe monolayers displayed substantial disassembly actin filaments wild-type (wt) NHERF1 but not NHERF1-Delta Ezrin-Radixin-Moesin (ERM) increased F-actin assembly organization....
Background: The loss of nigrostriatal neurons containing dopamine (DA) together with the “mitochondrial dysfunction” in midbrain represent two main causes related to symptoms Parkinson’s disease (PD). Hence, aim this investigation is co-administer missing DA and antioxidant grape seed-derived proanthocyanidins (grape seed extract, GSE) order increase levels neurotransmitter (which unable cross Blood Brain Barrier) reducing oxidative stress (OS) PD, respectively. Methods: For purpose, we...
This work aimed to evaluate the potential of nanosystems constituted by dopamine (DA) and antioxidant Citicoline (CIT) co-loaded in solid lipid nanoparticles (SLNs) for intranasal administration treatment Parkinson disease (PD). Such nanosystems, denoted as DA-CIT-SLNs, were designed according concept multifunctional nanomedicine where multiple biological roles are combined into a single nanocarrier prepared melt emulsification method employing self-emulsifying Gelucire® 50/13 matrix. The...
Microparticles (MPs) are membrane vesicles released during cell activation and apoptosis. MPs have different biological effects depending on the from they originate. Cystic fibrosis (CF) lung disease is characterized by massive neutrophil granulocyte influx in airways, their eventually We investigated presence phenotype of sputum, a rich non-invasive source inflammation biomarkers, acute stable CF adult patients. Spontaneous obtained 21 patients (10 11 stable) 7 with primary ciliary...
Cystic fibrosis (CF) is a monogenic disease caused by mutations in the CF transmembrane conductance regulator (CFTR) gene, with lung and liver manifestations. Because of pitfalls gene therapy, novel approaches for reconstitution airway epithelium CFTR expression should be explored. In present study, human amniotic mesenchymal stem cells (hAMSCs) were isolated from term placentas characterized phenotypic pluripotency markers, differentiation potential towards mesoderm (osteogenic adipogenic)...
ABSTRACT The most common mutation of the cystic fibrosis transmembrane regulator (CFTR) gene, F508del, produces a misfolded protein resulting in its defective trafficking to cell surface and an impaired chloride secretion. Pharmacological treatments partially rescue F508del CFTR activity either directly by interacting with mutant and/or indirectly altering cellular homeostasis. Here, we show that phosphorylation ezrin together binding phosphatidylinositol-4,5-bisphosphate (PIP2) tethers...
The role of colony stimulating factors (CSFs) in cystic fibrosis (CF) circulating neutrophils has not been thoroughly evaluated, considering that the neutrophil burden lung inflammation these subjects is very high. aim this study was to assess granulocyte-CSF (G-CSF) and granulocyte-macrophage-CSF (GM-CSF) levels CF patients various clinical conditions how cytokines impact on activation priming neutrophils. G-CSF GM-CSF were measured sputum serum samples stable (n = 21) with acute...
We have already formulated solid lipid nanoparticles (SLNs) in which the combination of neurotransmitter dopamine (DA) and antioxidant grape-seed-derived proanthocyanidins (grape seed extract, GSE) was supposed to be favorable for Parkinson's disease (PD) treatment. In fact, GSE supply would reduce PD-related oxidative stress a synergic effect with DA. Herein, two different methods DA/GSE loading were studied, namely, coadministration aqueous phase DA GSE, other approach consisting physical...
Abstract Cystic fibrosis ( CF ) is caused by mutations in the transmembrane conductance regulator CFTR gene, with most of mortality given lung disease. Human amniotic mesenchymal stromal (stem) cells (h AMSC s) hold great promise for regenerative medicine field disease; however, their potential as therapeutics disease has not been fully explored. In present study, h s were analysed co‐cultures on Transwell filters immortalized airway epithelial CFBE 41o‐ line) at different ratios to exploit...
The supply of nutrients, such as antioxidant agents, to fish cells still represents a challenge in aquaculture. In this context, we investigated solid lipid nanoparticles (SLN) composed combination Gelucire® 50/13 and Precirol® ATO5 administer grape seed extract (GSE) mixture containing several compounds. the two lipids for SLN formation resulted colloids exhibiting mean particle sizes range 139-283 nm zeta potential values +25.6-43.4 mV. Raman spectra X-ray diffraction evidenced structural...
// Arcangelo Liso 1 , Stefano Castellani Francesca Massenzio Rosa Trotta Alessandra Pucciarini 2 Barbara Bigerna Pasquale De Luca 3 Pietro Zoppoli 4 Filippo Castiglione 5 Maria Concetta Palumbo Fabrizio Stracci 6 Matteo Landriscina 1,7 Giorgina Specchia 8 Leon A. Bach 9,10 Massimo Conese and Brunangelo Falini Department of Medical Surgical Sciences, University Foggia, Italy Institute Haematology, Perugia, Stazione Zoologica Dohrn, Naples, Dipartimento di Medicina Sperimentale e Clinica,...