A5012656898- Microbial Community Ecology and Physiology
- RNA Research and Splicing
- Environmental DNA in Biodiversity Studies
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- CRISPR and Genetic Engineering
- Identification and Quantification in Food
- Cancer Research and Treatments
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Marine Ecology and Invasive Species
- Protease and Inhibitor Mechanisms
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Algal biology and biofuel production
- Protist diversity and phylogeny
- Cancer Cells and Metastasis
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- Animal Genetics and Reproduction
- Marine and coastal plant biology
- Aquaculture disease management and microbiota
- Effects and risks of endocrine disrupting chemicals
Stazione Zoologica Anton Dohrn
2014-2024
University of Coimbra
2024
University of Bari Aldo Moro
2018
Biogem
2004-2016
University of Turin
2011
Federico II University Hospital
2000-2005
Institute of Genetics and Biophysics
1996-2004
European Institute of Oncology
2000-2004
University of Naples Federico II
1992-2000
National Research Council
1998
Sp3 is a member of the Sp family transcription factors and binds to DNA with affinity specificity comparable that Sp1. We demonstrate bifunctional factor can both activate repress transcription. Gene fusion experiments in mammalian cells activation potential distributed over an extensive glutamine-rich N-terminal region, whereas repressor activity has been mapped 72-amino acid region located at 5′ zinc finger DNA-binding domain. demonstrated repression strictly dependent on context sites...
Recently, a family of transcription factors structurally related to Sp1 has been described; thus, more than one activator may bind the GC boxes present in number viral and cellular promoters. We have compared transactivation potentials Sp1, Sp3 Sp4 proteins on human immunodeficiency virus type 1 (HIV-1) promoter. The long terminal repeat (LTR) HIV-1 contains three binding sites for factor (GC boxes) which are involved both basal Tat-mediated transcriptional activation. Moreover, cooperative...
Congenital hypothyroidism with thyroid dysgenesis (TD) is a frequent human condition characterized by elevated levels of TSH in response to reduced hormone levels. genetically heterogeneous disease. In the majority cases studied, no causative mutations have been identified and very often disease does not show Mendelian transmission. However, approximately 5% cases, it can be consequence genes encoding receptor or transcription factors TITF1, FOXE1, PAX8. We report here that mouse models,...
Environmental DNA metabarcoding (eDNA metaB) is fundamental for monitoring marine biodiversity and its spread in coastal ecosystems. We applied eDNA metaB to seawater samples investigate the spatiotemporal variability of plankton small pelagic fish, comparing sites with different environmental conditions across a coast-to-offshore gradient at river mouths along Campania coast (Italy) over 2 years (2020-2021). found marked seasonality planktonic community regional scale, likely owing...
BACKGROUND: Alpha‐lipoic acid (ALA), is a potent antioxidant mitochondrial coenzyme, the trometamol salt of thioctic that has been shown in clinical studies to be neuroprotective. This study examined effect ALA on symptomatology Burning mouth syndrome (BMS). SUBJECTS AND METHODS: Forty‐two patients with BMS and no or laboratory evidence organic oral disease were divided into two groups (Test Control) each 21 subjects, matched for age sex. The Test group given (thioctic acid; Tiobec) 30 days,...
Malignant pleural effusions (MPEs) could represent an excellent source to culture a wide variety of cancer cells from different donors. In this study, we set up conditions for deriving MPEs several patients affected by the most frequent form lung cancer, namely subset non small cell cancers (NSCLC) classified as Lung Adenocarcinomas (AdenoCa) which account approximately 40% cases. AdenoCa malignant gave rise in vitro cultures both adherent and/or spheroid almost all cases analyzed. We...
MicroRNAs (miRNAs) are small non-coding RNAs involved in the modulation of gene expression and control numerous cell functions. Alterations miRNA patterns frequently occur cancer metabolic disorders, including obesity. Recent studies showed remarkable stability miRNAs both plasma serum making them suitable as potential circulating biomarkers for a variety diseases conditions. The aim this study was to assess profile expressed samples overweight or obese (OW/Ob) normal weight (NW) prepubertal...
Nearly 10 years ago, the World Health Organization reported increasing prevalence of overweight and obesity worldwide as a challenge for public health due to associated adverse consequences. Epidemiological studies established firm relationship between an elevated body mass index chronic conditions such diabetes, dyslipidemia, hypertension, heart disease, non-alcoholic fatty liver some types cancer. Omic demonstrated that microRNA (miRNA) profile changes in tissues correlate with number...
Sp3 is a member of the Sp family transcription factors, and it binds to GC box with an affinity specificity comparable that Sp1. Previous studies have shown repressed Sp1-mediated transcriptional activation, suggesting inhibitory family. The experiments described here demonstrate contains portable repression domain can function independently from zinc finger DNA-binding domain. We found amino-terminal region tethered promoter DNA by connecting heterologous protein represses activation...
While much experimental data shows that vaccination efficiently inhibits a subsequent challenge by transplantable tumor, its ability to inhibit the progress of autochthonous preneoplastic lesions is virtually unknown. In this article, we show combined DNA and cell vaccine persistently such in murine HER-2/neu mammary carcinogenesis model. At 10 weeks age, all ten gland samples from HER-2/neu–transgenic mice displayed foci hyperplasia progressed invasive tumors. Vaccination with plasmids...
The thyroid and lungs originate as neighboring bud shaped outgrowths from the midline of embryonic foregut. When how organ specific programs regulate development into structures distinct shapes, positions functions is incompletely understood. To characterize, at least in part, genetic basis these events, we have employed laser capture microdissection microarray analysis to define gene expression mouse lung primordia E10.5. By comparing transcriptome each that whole embryo well other, broadly...
Algal viruses are important contributors to carbon cycling, recycling nutrients and organic material through host lysis. Although viral infection has been described as a primary mechanism of phytoplankton mortality, little is known about defense responses. We show that the bloom-forming, planktonic diatom Chaetoceros socialis induces mass formation resting spores, heavily silicified life cycle stage associated with export due rapid sinking. RNA was detected within mature virions were not...
Mammalian homologues of Drosophila Seven in Absentia (SIAHs) target for proteasome-mediated degradation several factors involved cell growth and tumorigenesis. Here we show that SIAH-1/2 binds targets the putative tumor suppressor tripartite motif (TRIM) family member PML, leading to loss its transcriptional co-activating properties a reduction number endogenous PML nuclear bodies. Association with requires substrate-binding domain (SBD) through an interacting surface apparently distinct...
Abstract Background The transcription factor Nkx2-1 (also known as TTF-1, Titf1 or T/EBP) contains two apparently redundant activation domains and is post-translationally modified by phosphorylation. We have generated mouse mutant strains to assess the roles of phosphorylation in development differentiation. Results Mouse expressing variants deleted either domain been constructed. Phenotypic analysis shows for each a distinct set defects demonstrating that portions protein endow diverse...
Regulated intramembrane proteolysis of the beta-amyloid precursor protein by gamma-secretase yields two peptides. One, amyloid-beta, is major component amyloid plaques found in Alzheimer's disease patients. The other, APP IntraCellular Domain, has been involved regulation apoptosis, calcium flux and gene transcription. To date, a few potential target genes transcriptionally controlled AID, alone or complexed with Fe65/Tip60, have described. Although reports are controversial: these include...
Abstract A broad diversity of sex-determining systems has evolved in eukaryotes. However, information on the mechanisms sex determination for unicellular microalgae is limited, including diatoms, key-players ocean food webs. Here we report identification a mating type (MT) determining gene diatom Pseudo-nitzschia multistriata . By comparing expression profile two MTs, find five MT-biased genes, which one, MRP3 , expressed exclusively MT+ strains monoallelic manner. short tandem repeat...