In the attempt to find valid alternatives classic antibiotics and in view of current limitations efficacy antimicrobial-coated or loaded biomaterials, this work is focused on development a new glass-ceramic with antibacterial performance together safe biocompatibility. This bactericidal composed combeite nepheline crystals residual glassy matrix has been obtained using an antimicrobial soda-lime glass as precursor. Its inhibitory effects bacterial growth biofilm formation were proved against...

In order to explore the pharmacodynamic need for continuous versus intermittent (three times a day) administration of ceftazidime in critically ill patients, pharmacokinetic computerized device was used simulate concentrations human serum after 6 g/day.Efficacy measured as capability simulated over time reduce initial inoculum against four strains Pseudomonas aeruginosa. MICs matched NCCLS breakpoints: one susceptible strain (MIC = 8 mg/L), two intermediate 16 mg/L) and resistant 32 mg/L)....

This paper reports the effect of soda-lime-glass-nAg coating on viability an in vitro biofilm Streptococcus oralis. Three strains (ATCC 35037 and two clinical isolates from periodontitis patients) were grown coated with glass, glass containing silver nanoparticles, uncoated titanium alloy disks. Two different methods used to quantify formation abilities: crystal violet staining determination viable counts. The influence surface morphology cell attachment was studied. characterized by...

To explore the pharmacokinetics (PK) and pharmacodynamics (PD) of micafungin in patients undergoing continuous venovenous haemofiltration (CVVH). Ten receiving CVVH treated with 100 mg/day were included (April–December 2012). was performed using polyethersulphone or polysulphone haemofilters. Dialysis membranes not changed on sampling days. On Days 1 2, blood samples from arterial pre-filter venous post-filter ports ultrafiltrate collected at start end infusion 3, 5, 8, 18 24 h....

ObjectivesTo explore serum and tissue pharmacodynamics of linezolid versus vancomycin against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates with different MBC/MIC ratios.

We aimed to evaluate the effects of ultrasound-assisted wound (UAW) debridement on cellular proliferation and dermal repair in complicated diabetic foot ulcers as compared receiving surgical/sharp debridement. A randomized controlled trial was performed involving 51 outpatients with that either received surgical (n = 24) or UAW 27) every week during a six-week treatment period. Compared patients debridement, treated exhibited significantly improved proliferation, determined by CD31 staining,...

Bactericidal activity depends on antibiotic-bacteria couples, resistance phenotype and theoretically protein binding. This work explores the influence of binding bactericidal two antibiotics, daptomycin versus vancomycin, that exhibit, respectively, different C(max) (56 25.5 mg/L), (91.7% 36.9%) thus theoretical free-drug fractions (4.7 16.1 mg/L).The effect presence physiological concentrations human albumin (4 g/dL) or serum (90%) was studied against Gram-positive isolates with troublesome...

Objective: To evaluate the clinical and microbiological effects of sequential wound debridement in a case series neuroischaemic diabetic foot ulcers (DFUs) using an ultrasound-assisted (UAW) device. Method: A prospective, single-centre study, involving 24 DFUs, was conducted to with UAW during six-week treatment period. Soft tissue punch biopsies were taken every second week treatment, both before after sessions. Qualitative quantitative analysis performed wounds assessed at patient...

This study aimed to estimate the prevalence of methicillin-susceptible and -resistant Staphylococcus aureus (MSSA MRSA) methicillin-resistant epidermidis (MRSE) nasopharyngeal carriage among Doctors Podiatric Medicine (Podiatrists) determine potential risk factors.A cross-sectional was carried out in 2016-2017 239 podiatrists Spain. The presence MSSA, MRSA, MRSE determined by microbiological analysis nasal exudate antimicrobial susceptibility determined. Each podiatrist completed a...

The methods that are used for the diagnostic confirmation of human papillomavirus (HPV) include excisional biopsy and histopathological studies or polymerase chain reaction (PCR). They invasive, laborious, subject to ethical restrictions due benign nature these warts. This study aims analyse accuracy noninvasive swab samples diagnose plantar Fifty warts were included in study. Skin swabs hyperkeratosis skin scales collected from each wart. Multiplex PCR was performed detect type HPVs....

The use of an antimicrobial dressing instead a non-antimicrobial may increase the number diabetic foot ulcers healed over medium-term follow-up period. study aim was to evaluate clinical and microbiological efficacy silver foam in management ulcers. We conducted single-center, prospective, open, noncontrolled involving 21 outpatients with mild infection. All patients received standard care for their wounds silicone adhesive applied twice per week wound during 6-week treatment Soft tissue...

Aims: The aim of this study was to analyse the nucleotide sequences regions encoding penicillin-binding domains pbp1A, pbp2B and pbp2X genes murM alleles from 14 selected amoxicillin-resistant Streptococcus pneumoniae isolates (MICs 8–16 mg/L) obtained in Spain.

ObjectivesAttempts to interpret antibiotic pharmacodynamics using reported protein binding may underestimate true activity. To elucidate this issue we examined bacterial killing kinetics at cefditoren concentrations equal Cmax in the presence of 90% human serum or albumin physiological concentrations.

The surface adhesion of bacterial cells and the in vivo biocompatibility a new ceramic–metal composite made zirconium dioxide tantalum were evaluated. Within framework an vitro study using crystal violet staining colony counting methods, relatively similar Streptococcus oralis to 3Y-TZP/Ta biocermet (roughness Ra = 0.12 ± 0.04 µm) Ti-Al6-V4 titanium alloy (Ra 0.01 was found. In addition, preliminary focused on histological analysis series rods implanted jaws beagle dogs for six-month period,...

Objectives: To investigate the azithromycin pharmacodynamic parameters predicting bacterial killing in epithelial lining fluid (ELF) versus serum against macrolide-susceptible and -resistant Streptococcus pneumoniae isolates (with different resistance genotypes), through simulation of concentrations achieved after a 500 mg intravenous (iv) once day regimen. Methods: An vitro computer-controlled human ELF was carried out, colony counts were determined over 24 h. Four strains with MIC values...

Background Specific antibodies are likely to be present before S. pneumoniae infection. We explored cefditoren (CDN) total and free values of serum concentrations exceeding the MIC (t>MIC) related efficacy in a mice sepsis model, effect specific gammaglobulins on in-vitro phagocytosis in-vivo efficacy. Methodology/Principal Findings used three pneumococcal isolates (serotype, CDN): Strain 1 (6B, µg/ml), 2 (19F, µg/ml) 3 (23F, 4 µg/ml). Hyperimmune (HS) was obtained from immunized with...

Background Specific antibodies mediate humoral and cellular protection against invading pathogens such as Streptococcus pneumoniae by activating complement mediated immunity, promoting phagocytosis stimulating bacterial clearance. The emergence of pneumococcal strains with high levels antibiotic resistance is great concern worldwide a serious threat for public health. Methodology/Principal Findings Flow cytometry was used to determine whether complement-mediated immunity three...

Objectives: To investigate the bactericidal activity against Streptococcus pneumoniae of simulated amoxicillin serum concentrations obtained in humans after 2000/125 mg sustained-release (SR) and 875/125 co-amoxiclav administered twice three times a day, respectively.

Among 165 Spanish Haemophilus influenzae isolates with mutations in the ftsI gene (ftsI(+)) (2005 to 2007), 73% were beta-lactamase negative and 26.7% positive. The proportion of beta-lactamase-negative beta-lactamase-positive was 2:1 4:1 general, versus 1:3 pediatric hospitals. 44 strains, 8 strains produced ROB-1 (5 from hospital). beta-Lactamase-positive ftsI(+) phylogenetically closer than strains.

The tigecycline susceptibility of six different Enterobacteriaceae strains with reported high MICs was determined in quintuplicate by four methodologies using Mueller-Hinton agar and broth from manufacturers. Etest were a >or=1-fold dilution lower than those microdilution dilution, the highest modal values given dilution. obtained Oxoid medium, lowest inhibition zone (disc diffusion) bioMérieux media. Difco or Merck

The benefit of autologous platelet-rich plasma (PRP) treatment is still under discussion. Variations in PRP products, consequence the lack a standardised protocol for multitude commercially available blood separation systems and knowledge optimal composition or its suitability proposed indication are some reasons behind clinical inconsistencies. impact inter-subject variability has received less attention comparison. purpose this study was to determine variability, based on age, concentrates...