A5049333278- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Viral gastroenteritis research and epidemiology
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Vibrio bacteria research studies
- Viral Infections and Vectors
- Protein Structure and Dynamics
- Mosquito-borne diseases and control
- Viral Infections and Immunology Research
- Toxin Mechanisms and Immunotoxins
- Enzyme Structure and Function
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Outbreaks Research
- Antimicrobial Peptides and Activities
- Monoclonal and Polyclonal Antibodies Research
- Animal Disease Management and Epidemiology
- interferon and immune responses
- Hepatitis B Virus Studies
- Glycosylation and Glycoproteins Research
- Biochemical and Structural Characterization
- Escherichia coli research studies
- HIV Research and Treatment
- Animal Virus Infections Studies
- Photosynthetic Processes and Mechanisms
Tsinghua University
2016-2025
Shanxi Medical University
2024-2025
Center for Life Sciences
2022-2025
Chengdu Third People's Hospital
2025
Southwest Jiaotong University
2025
King Center
2024-2025
Beijing Advanced Sciences and Innovation Center
2016-2024
Sichuan Agricultural University
2023
Indiana University School of Medicine
2020
Purdue University West Lafayette
2006-2015
The trimeric SARS coronavirus (SARS-CoV) surface spike (S) glycoprotein consisting of three S1-S2 heterodimers binds the cellular receptor angiotensin-converting enzyme 2 (ACE2) and mediates fusion viral membranes through a pre- to postfusion conformation transition. Here, we report structure SARS-CoV S in complex with its host cell ACE2 revealed by cryo-electron microscopy (cryo-EM). shows that only one receptor-binding domain adopts protruding "up" conformation. In addition, studied...
The global outbreak of SARS in 2002-2003 was caused by the infection a new human coronavirus SARS-CoV. SARS-CoV is mediated mainly through viral surface glycoproteins, which consist S1 and S2 subunits form trimer spikes on envelope virions. Here we report ectodomain structures spike different conformational states determined single-particle cryo-electron microscopy. conformation 1 at 4.3 Å resolution three-fold symmetric has all three receptor-binding C-terminal domain (CTD1s) "down"...
Antibodies block Ebola virus entry The recent outbreak in West Africa illustrates the need for both an effective vaccine and therapies to treat infected individuals. Corti et al. isolated two monoclonal antibodies from a survivor of 1995 Kikwit demonstrated their therapeutic efficacy virus–infected macaques. In fact, one antibody protected macaques when it was given up 5 days after infection. Misasi solved crystal structures fragments bound glycoprotein (GP), which mediates viral cell entry....
A 5.3 Å resolution, cryo-electron microscopy (cryoEM) map of Chikungunya virus-like particles (VLPs) has been interpreted using the previously published crystal structure E1-E2 glycoprotein heterodimer. The heterodimer was divided into domains to obtain a good fit cryoEM density. Differences in T = 4 quasi-equivalent components show their adaptation different environments. spikes on icosahedral 3-fold axes and those general positions are significantly different, possibly representing phases...
C-type lectins are a family of proteins with carbohydrate-binding activity. Several in mammals or arthropods employed as receptors attachment factors to facilitate flavivirus invasion. We previously identified lectin Aedes aegypti, designated mosquito galactose specific lectin-1 (mosGCTL-1), facilitating the West Nile virus (WNV) on cell membrane. Here, we first that 9 A. aegypti mosGCTL genes were key susceptibility DENV-2 infection, which mosGCTL-3 exhibited most significant effect. found...
Abstract The structure and assembly of bacteriophage T4 has been extensively studied. However, the detailed portal protein remained unknown. Here we report assembly, gene product 20 (gp20), determined by cryo-electron microscopy (cryo-EM) to 3.6 Å resolution. In addition, analysis a 10 resolution cryo-EM map an empty prolate head shows how dodecameric interacts with capsid gp23 at special pentameric vertex. gp20 also verifies that is required for initiating attachment packaging motor,...
Abstract DNA nanostructures with increasing complexity have showcased the power of programmable self-assembly from strands. At nascent stage field, a variety small branched objects consisting few strands were created. Since then, quantum leap has been achieved by scaffolded ‘origami’ approach and scaffold-free using single-stranded tiles/bricks—creating fully addressable two-dimensional three-dimensional designed on densely packed lattices. Recently, wireframe architectures applied to...
Abstract Mycobacterium tuberculosis (Mtb) exposure drives antibody responses, but whether patients with active elicit protective antibodies, and against which antigens, is still unclear. Here we generate monoclonal antibodies from memory B cells of one patient to investigate the cell responses during infection. The members four distinct clones, are directed Mtb phosphate transporter subunit PstS1. Antibodies p4-36 p4-163 reduce bovis- BCG levels in an ex vivo human whole blood growth...
Abstract The respiratory system, especially the lung, is key site of pathological injury induced by SARS-CoV-2 infection. Given low feasibility targeted delivery antibodies into lungs intravenous administration and short half-life period in intranasal or aerosolized immunization, mRNA encoding broadly neutralizing with lung-targeting capability can perfectly provide high-titer to prevent Here, we firstly identify a human monoclonal antibody, 8-9D, broad potency against variants....
Eastern Equine Encephalitis virus (EEEV) is an alphavirus that can cause severe diseases in infected humans. The very low-density lipoprotein receptor (VLDLR) was recently identified as a of EEEV. Herein, we performed cryo-electron microscopy structural and biochemistry studies on the specific interactions between EEEV VLDLR. Our results show VLDLR binds at three different sites A, B C through its membrane-distal LDLR class A (LA) repeats. Site located cleft E1-E2 heterodimers. near...
Tailed DNA bacteriophages assemble empty procapsids that are subsequently filled with the viral genome by means of a packaging machine situated at special fivefold vertex. The consists “small terminase” and “large component. One functions small terminase is to initiate genome, whereas large responsible for ATP-powered translocation DNA. subunit has three domains, an N-terminal DNA-binding domain, central oligomerization C-terminal domain interacting terminase. Here we report structures in...
A cryoelectron microscopy 8.5 Å resolution map of the 1,900 diameter, icosahedral, internally enveloped Paramecium bursaria chlorella virus was used to interpret structures at initial stages cell infection. fivefold averaged demonstrated that two minor capsid proteins involved in stabilizing are missing vicinity unique vertex. Reconstruction presence host walls established spike vertex initiates binding wall, which results nucleocapsid moving closer cell. This process is concurrent with...
“Sputnik” is a dsDNA virus, referred to as virophage, that coassembled with Mimivirus in the host amoeba. We have used cryo-EM produce an electron density map of icosahedral Sputnik virus at 3.5-Å resolution, sufficient verify identity most amino acids capsid proteins and establish pentameric protein forming fivefold vertices. It was also shown lacks internal membrane. The organized into T = 27 lattice which there are 260 trimeric capsomers 12 capsomers. consist three double “jelly-roll”...
The mature virion of the tailed bacteriophage ϕ29 is an ~33 MDa complex that contains more than 450 subunits seven structural proteins assembling into a prolate head and short non-contractile tail. Here, we report near-atomic structures pre-genome packaging (prohead), genome-emptied virion. Structural comparisons suggest local rotation or oscillation head-tail connector upon DNA release. Termination occurs through pressure-dependent correlative positional conformational changes in connector....
Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and minor proteins pIII, pVII, pVI, pIX. Here, we report cryo-EM structure of a ~500 Å long bacteriophage M13 mini variant. The distal ends phage are sealed by two cap-like complexes composed proteins. top cap complex consists pVII pIX, both exhibiting helix structure. Arg33 Glu29 located on inner surface cap, play key role in recognizing packaging signal. bottom is formed...