A5091037351- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- COVID-19 Clinical Research Studies
- Sepsis Diagnosis and Treatment
- Immune Cell Function and Interaction
- Ubiquitin and proteasome pathways
- Cancer Immunotherapy and Biomarkers
- Respiratory Support and Mechanisms
- Cancer Genomics and Diagnostics
- Pancreatic function and diabetes
- Lung Cancer Treatments and Mutations
- PI3K/AKT/mTOR signaling in cancer
- Pancreatic and Hepatic Oncology Research
- Melanoma and MAPK Pathways
- Drug Transport and Resistance Mechanisms
- Hepatocellular Carcinoma Treatment and Prognosis
- Galectins and Cancer Biology
- Biosimilars and Bioanalytical Methods
- Synthesis of Tetrazole Derivatives
- Radiomics and Machine Learning in Medical Imaging
- Protein purification and stability
- Hematopoietic Stem Cell Transplantation
- Molecular Biology Techniques and Applications
- Adenosine and Purinergic Signaling
- PARP inhibition in cancer therapy
Centre Hospitalier Universitaire Amiens-Picardie
2016-2025
Centre Hospitalier Universitaire de Nantes
2024
Inserm
2016-2024
Université de Rennes
2019-2024
Oncogenesis Stress Signaling
2016-2024
Centre Eugène Marquis
2016-2023
Université de Picardie Jules Verne
2016-2021
Chirurgie et extrémité céphalique, caractérisation morphologique et fonctionnelle
2020
Centre Hospitalier Universitaire Henri-Mondor
2016
Université Toulouse III - Paul Sabatier
2016
Sorafenib, a kinase inhibitor active against various solid tumours, induces oxidative stress and ferroptosis, new form of necrosis, in some cancer cells. Clinically-applicable biomarkers that reflect the impact sorafenib on redox metabolism cells are lacking.We used gene expression microarrays, real-time PCR, immunoblot, protein-specific ELISA, reporter constructs encoding enzyme luciferase to study response panel sorafenib. Tumour explants prepared from surgical hepatocellular carcinoma...
Inositol-requiring enzyme 1 (IRE1) is a major mediator of the unfolded protein response (UPR), which activated upon endoplasmic reticulum (ER) stress. Tumor cells experience ER stress due to adverse microenvironmental cues, overcome by relying on IRE1 signaling as an adaptive mechanism. Herein, we report discovery structurally new inhibitors identified through structural exploration its kinase domain. Characterization in vitro and cellular models showed that they inhibit sensitize...
Sorafenib is the first line treatment for advanced hepatocellular carcinoma (HCC). We explored its impact on proteostasis of cancer cells, i.e. processes that regulate synthesis, maturation and turn-over cellular proteins. observed sorafenib inhibits production tumour marker alpha-foetoprotein (AFP) in two different HCC cell lines, an effect correlated with a radical inhibition protein biosynthesis. This was at clinically relevant concentrations not related to transport amino acids across...
Background/Objectives: Circulating tumor DNA (ctDNA) analysis is a powerful tool for non-invasive monitoring of burden and treatment response. Reliable quantification methods are critical the effective use ctDNA as biomarker. Digital PCR (dPCR) offers high sensitivity quantification, but requires prior knowledge tumor-specific genomic alterations. Next-generation sequencing (NGS) provides more comprehensive approach semi-quantitative, relying on variant allelic fraction (VAF), which can be...
Background: Determining the concentration of an antimicrobial agent in epithelial lining fluid using therapeutic drug monitoring (TDM) may be clinically useful for optimizing exposure patients with severe pneumonia. The can measured samples obtained during bronchoscopy and bronchoalveolar lavage (BAL). To account dilution induced by saline instillation procedure, BAL is usually corrected serum-to-BAL ratio urea. However, this analysis to integrated into daily TDM, a rapid, sensitive,...
The squamous cell carcinoma antigen (SCCA), encoded by the genes SERPINB3/B4, is a tumour marker produced head and neck (HNSCC). We aimed to examine SERPINB3/B4 mRNA levels its clinical significance in therapeutic context.We retrieved expression levels, clinical, pathological genomic data for 520 HNSCC from Cancer Genome Atlas (TCGA).HNSCC tumours express high of mRNA. SERPINB3 differs depending on Human papillomavirus (HPV) infection status, primary location, grade differentiation,...
Vemurafenib (Zelboraf) is a new tyrosine kinase inhibitor that selectively targets activated BRAF V600E gene and indicated for the treatment of advanced mutation-positive melanoma. We developed simple method vemurafenib quantification using liquid chromatography-tandem mass spectrometry. A stability study in human plasma was also performed.(13)C(6)-vemurafenib used as internal standard. single-step protein precipitation sample preparation. Chromatography performed on an Acquity UPLC system...
Abstract Purpose To assess the diagnostic performance of a panel standard tumor markers (TMs) in patients hospitalized with significant involuntary weight loss (IWL) and elevated levels inflammation biomarkers, combination TM finding computed tomography (CT) scan. Methods We conducted retrospective study internal medicine department at Amiens-Picardie University Medical Center (Amiens, France) between January 1st, 2015, November 2021. The inclusion criteria were age 18 or over, IWL (≥ 5 kg...
Uric acid (UA) is the end product of catabolism purines, and its serum levels are commonly increased in cancer patients. We aimed to explore transcriptional regulation tumour uricogenesis human tumours, relate with pathological pharmacological findings. Using data from The Cancer Genome Atlas (TCGA), we analysed expression xanthine dehydrogenase (XDH) adenine phosphoribosyltransferase (APRT), two key enzymes UA production purine salvage pathway, respectively. found large differences between...
Background/Aim: Organ transplant patients treated with the immunosuppressive drug cyclosporine A often present malignant kidney tumors. Cyclosporine can promote oncogenesis in a cell-intrinsic manner by increasing production of vascular endothelial growth factor (VEGF). Materials and Methods: We explored impact role unfolded protein response (UPR) on three human renal cell carcinoma (RCC) lines under normoxic hypoxic (1% O<sub>2</sub>) conditions. Results: regulated expression VEGF at...
Abstract One of the main glioblastoma (GB) features is diffuse migration tumor cells within surrounding brain parenchyma, rendering almost impossible complete resection and irradiation, leading to inexorable lethal relapse disease. In past years, we demonstrated that IRE1α (hereafter IRE1), one Endoplasmic Reticulum (ER) stress sensors, plays a key role in GB biology by impacting on immune infiltration, angiogenesis cell migration/invasion, all these being linked an alteration protein...
<title>Abstract</title> <bold>Background</bold>: Lung cancer treatment is a constantly evolving field, with an acceleration of innovative therapies over the last decade. Patients included in clinical trials are highly selected, thus efficacy all comers still scarce. Despite significant progress, mortality remains high, disease progression occurs most patients, and mechanisms underlying resistance to remain poorly understood. The BREATHE project (Biocollection for Research Thoracic Cancer)...
Background/Aim: Multiple myeloma (MM) is characterized by high production of immunoglobulins resulting in a constant source endoplasmic reticulum (ER)-stress. Mesencephalic astrocyte-derived neurotrophic factor (MANF) was identified as possible circulating biomarker that could help monitoring ER-stress mediated diseases. Materials and Methods: To assess the relevance MANF MM, we performed silico vitro analysis malignant cell lines including line RPMI 8226. Serum concentration compared...
The survival of patients with head and neck squamous cancer locoregional recurrence is short if salvage surgery or radiation cannot be performed. Systemic chemotherapy based on platinum salts cetuximab produces only partial transient responses. Immune checkpoint inhibitors (i.e., nivolumab) lead to a low complete response rate about 10%, but in some cases the effects can long-lasting. Intratumoral (ITC) has been proposed for local cell carcinoma an objective 27–50%. However, it often leads...