A5103017255- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Geophysics and Sensor Technology
- Genomics and Chromatin Dynamics
- RNA Interference and Gene Delivery
- RNA Research and Splicing
- Advanced Frequency and Time Standards
- Antibiotic Resistance in Bacteria
- Plant Virus Research Studies
- Neurobiology and Insect Physiology Research
- Hair Growth and Disorders
- Earthquake Detection and Analysis
- Phytoestrogen effects and research
- Inertial Sensor and Navigation
- Ion Channels and Receptors
- RNA and protein synthesis mechanisms
- Advanced MEMS and NEMS Technologies
- Spectroscopy and Quantum Chemical Studies
- Lipid Membrane Structure and Behavior
- Antimicrobial agents and applications
- Ion channel regulation and function
- Antimicrobial Peptides and Activities
- Protein Structure and Dynamics
Center for Excellence in Molecular Plant Sciences
2021-2024
Chinese Academy of Sciences
2011-2024
Huazhong University of Science and Technology
2024
University of Reading
2024
University of Chinese Academy of Sciences
2021-2023
China Medical University
2015
First Hospital of China Medical University
2015
Guangzhou Institutes of Biomedicine and Health
2011
DNA methylation affects gene expression and maintains genome integrity. The DNA-dependent RNA polymerase IV (Pol IV), together with the RNA-dependent RDR2, produces double-stranded small interfering precursors essential for establishing maintaining in plants. We determined cryo–electron microscopy structures of Pol IV–RDR2 holoenzyme backtracked transcription elongation complex. These reveal that RDR2 form a complex their active sites connected by an interpolymerase channel, through which...
Abstract De novo DNA methylation in plants relies on transcription of RNA polymerase V (Pol V) along with KTF1, which produce long non-coding RNAs for recruitment and assembly the machinery. Here, we report a cryo-EM structure Pol elongation complex bound to KTF1. The reveals conformation structural motifs active site that accounts its inferior RNA-extension ability. also features prevent it from interacting factors II IV. KOW5 domain KTF1 binds near exit channel providing scaffold proposed...
DNA-dependent RNA polymerases (Pols) transfer the genetic information stored in genomic DNA to all organisms. In eukaryotes, typical products of nuclear Pol I, II, and III are ribosomal RNAs, mRNAs, respectively. Intriguingly, plants possess two additional Pols, IV V, which produce small RNAs long noncoding respectively, mainly for silencing transposable elements. The five plant Pols share some subunits, but their distinct functions stem from unique subunits that interact with specific...
RNA polymerase IV (Pol IV) forms a complex with RNA-directed 2 (RDR2) to produce double-stranded (dsRNA) precursors essential for plant gene silencing. In the “backtracking-triggered channeling” model, Pol backtracks and delivers its transcript’s 3′ terminus RDR2, which synthesizes dsRNA. However, mechanisms underlying backtracking protection from cleavage are unclear. Here, we determined cryo–electron microscopy structures of elongation complexes at four states nucleotide addition cycle...
Depletion of intracellular Ca(2+) stores evokes store-operated entry through the release-activated (CRAC) channels. In this study, we found that was inhibited by neomycin, an aminoglycoside strongly binds phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). Patch clamp recordings revealed neomycin blocked CRAC currents reconstituted co-expression Orai1 and Stim1 in HEK293 cells. Using a rapamycin-inducible PtdIns(4,5)P2-specific phosphatase (Inp54p) system to manipulate PtdIns(4,5)P2...
Abstract De novo DNA methylation in plants relies on transcription elongation of RNA polymerase V (Pol V) along with KTF1 and SPT4, which produces long non-coding RNAs for recruitment assembly machinery. Here, we report a cryo-EM structure the KTF1-bound Pol complex. The reveals conformation motifs active site that accounts its inferior RNA-extension ability. further structural features prevent it from interacting general factors II IV. KOW5 domain binds near exit channel V, where provides...