A5024840454- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- RNA modifications and cancer
- Antibiotic Resistance in Bacteria
- Genetic diversity and population structure
- ATP Synthase and ATPases Research
- Genomics and Phylogenetic Studies
- Plant Diversity and Evolution
- Mitochondrial Function and Pathology
- Cancer-related molecular mechanisms research
- Photosynthetic Processes and Mechanisms
- Plant and Fungal Species Descriptions
- Tuberculosis Research and Epidemiology
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Enzyme Structure and Function
- Epigenetics and DNA Methylation
- Plant and animal studies
- Mycobacterium research and diagnosis
- Natural product bioactivities and synthesis
- Bacterial biofilms and quorum sensing
- Chromosomal and Genetic Variations
- Vibrio bacteria research studies
- Genetic Mapping and Diversity in Plants and Animals
Zhejiang University
2012-2025
Sir Run Run Shaw Hospital
2019-2025
Shanghai University of Traditional Chinese Medicine
2025
Chinese Academy of Sciences
2014-2024
Chengdu Institute of Biology
2022-2024
Nanjing University
2024
Central Hospital of Zibo
2024
Pudong New Area People's Hospital
2024
China Institute of Veterinary Drug Control
2023-2024
Blood Center of Zhejiang Province
2024
During transcription initiation, RNA polymerase (RNAP) binds and unwinds promoter DNA to form an RNAP-promoter open complex. We have determined crystal structures at 2.9 3.0 Å resolution of functional initiation complexes comprising Thermus thermophilus polymerase, σ(A), a fragment corresponding the bubble downstream double-stranded The show that σ recognizes -10 element discriminator through interactions include unstacking insertion into pockets three bases RNAP -4/+2 region pocket +2 base....
Abstract Reading and writing are crucial life skills but roughly one in ten children affected by dyslexia, which can persist into adulthood. Family studies of dyslexia suggest heritability up to 70%, yet few convincing genetic markers have been found. Here we performed a genome-wide association study 51,800 adults self-reporting diagnosis 1,087,070 controls identified 42 independent significant loci: 15 genes linked cognitive ability/educational attainment, 27 new potentially more specific...
Class II transcription activators function by binding to a DNA site overlapping core promoter and stimulating isomerization of an initial RNA polymerase (RNAP)-promoter closed complex into catalytically competent RNAP-promoter open complex. Here, we report 4.4 angstrom crystal structure intact bacterial class activation The comprises Thermus thermophilus activator protein TTHB099 (TAP) [homolog Escherichia coli catabolite (CAP)], T. RNAP σ(A) holoenzyme, TAP-dependent promoter,...
Abstract Purpose: PI3K and STAT3 are frequently activated in cancer progression. However, little is known about the underlying mechanisms by which regulate head neck squamous cell (HNSCC) growth. The lncRNA HOX transcript antisense RNA (HOTAIR) was found to modulate progression of HNSCC. In this study, we attempted establish correlation PI3K/STAT3/HOTAIR signaling with HNSCC its sensitivity toward platinum-based targeted anti-EGFR combination therapy. Experimental Design: We first analyzed...
Abstract As one of the great survivors plant kingdom, barnyard grasses ( Echinochloa spp.) are most noxious and common weeds in paddy ecosystems. Meanwhile, at least two species have been domesticated cultivated as millets. In order to better understand genomic forces driving evolution toward weed crop characteristics, we assemble genomes three (allohexaploid E. crus-galli colona , allotetraploid oryzicola ) re-sequence 737 accessions millets from 16 rice-producing countries. Phylogenomic...
In response to DNA damage, bacterial RecA protein forms filaments with the assistance of DinI protein. The stimulate autocleavage LexA, repressor more than 50 SOS genes, and activate response. During late phase response, UmuD λ CI, leading mutagenic repair lytic cycle, respectively. Here, we determined cryo-electron microscopy structures Escherichia coli in complex DinI, UmuD, λCI by helical reconstruction. reveal that LexA dimers bind filament groove cleave an intramolecular intermolecular...
We have previously demonstrated that the V0 domain of coated vesicle V-ATPase, a 250-kDa integral complex, does not form functional proton channel (Zhang, J., Myers, M., and Forgac, M. (1992) J. Biol. Chem. 267, 9773-9778). In present study we describe dissociation complex separation subunits by gel filtration. Dicyclohexylcarbodiimide-inhibitable passive conductance reconstituted vesicles containing reassembled was measured in response to K+/valinomycin-generated membrane potential....
We have previously demonstrated that the coated vesicle vacuolar H(+)-ATPase (V-ATPase) can be inactivated by formation of intramolecular disulfide bonds (Feng, Y., and Forgac, M. (1992) J. Biol. Chem. 267, 19769-19772). The bond responsible for inactivation distinguished from other form fact inactivating is blocked ATP or high ionic strength. By taking advantage these properties, we selectively labeled ATPase at relevant cysteine residues with fluorescein maleimide. After analyzing...
Previous studies have shown an anticancer effect of vitamin D, but the mechanisms underlying this action not been fully explored. Here we show that 1,25-dihydroxyvitamin D3 (VD3, active form D) significantly promoted apoptosis in undifferentiated gastric cancer cell line HGC-27, and was accompanied by a concurrent increase phosphatase tensin homolog deleted on chromosome 10 (PTEN) expression VD3 treatment. In contrast, knockdown PTEN stable transfection small interfering RNA greatly...
We report that bacterial RNA polymerase (RNAP) is the functional cellular target of depsipeptide antibiotic salinamide A (Sal), and we Sal inhibits RNAP through a novel binding site mechanism. show synthesis in cells mutations confer Sal-resistance map to genes. interacts with active-center ‘bridge-helix cap’ comprising N-terminal hinge’, ‘F-loop’, ‘link region’. nucleotide addition transcription initiation elongation. present crystal structure defines interactions between effects on...
Using a combination of genetic, biochemical, and structural approaches, we show that the cyclic-peptide antibiotic GE23077 (GE) binds directly to bacterial RNA polymerase (RNAP) active-center ‘i’ ‘i+1’ nucleotide binding sites, preventing initiating nucleotides, thereby transcription initiation. The target-based resistance spectrum for GE is unusually small, reflecting fact site on RNAP includes residues active center cannot be substituted without loss activity. different from rifamycin...
Abstract Background Common walnut ( Juglans regia L.) is one of the top four most consumed nuts in world due to its health benefits and pleasant taste. Despite economic importance, evolutionary history genetic control adaptation agronomic traits remain largely unexplored. Results We report a comprehensive genomic variation map based on whole-genome resequencing 815 accessions. Evolutionary analyses suggest that Chinese J. diverged from sigillata with extensive hybridizations after split two...
Abstract LIN28 is an RNA binding protein with important roles in early embryo development, stem cell differentiation/reprogramming, tumorigenesis and metabolism. Previous studies have focused mainly on its role the cytosol where it interacts Let-7 microRNA precursors or mRNAs, few addressed LIN28’s within nucleus. Here, we show that displays dynamic temporal spatial expression during murine development. Maternal drops upon exit from 2-cell stage, zygotic induced at forming nucleolus 4-cell...
We have recently demonstrated that Cys-254 of the 73-kDa A subunit clathrin-coated vesicle (H+)-ATPase is responsible for sensitivity enzyme to sulfhydryl reagents (Feng, Y., and Forgac, M. (1992) J. Biol. Chem. 267, 5817-5822). In present study we observe purified enzyme, disulfide bond formation causes inactivation proton transport which reversed by dithiothreitol (DTT). DTT also restores activity oxidized following treatment with N-ethylmaleimide (NEM). These results indicate between...
Abstract Bacteriophage Q protein engages σ-dependent paused RNA polymerase (RNAP) by binding to a DNA site embedded in late gene promoter and renders RNAP resistant termination signals. Here, we report single-particle cryo-electron microscopy (cryo-EM) structure of an intact Q-engaged arrested complex. The reveals key interactions responsible for pause, engagement, Q-mediated transcription antitermination. shows that two protomers (Q I II ) bind direct-repeat contact distinct elements the...
DNA methylation affects gene expression and maintains genome integrity. The DNA-dependent RNA polymerase IV (Pol IV), together with the RNA-dependent RDR2, produces double-stranded small interfering precursors essential for establishing maintaining in plants. We determined cryo–electron microscopy structures of Pol IV–RDR2 holoenzyme backtracked transcription elongation complex. These reveal that RDR2 form a complex their active sites connected by an interpolymerase channel, through which...