A5019346237- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Reproductive biology and impacts on aquatic species
- Animal Genetics and Reproduction
- Aquaculture Nutrition and Growth
- Growth Hormone and Insulin-like Growth Factors
- Chromosomal and Genetic Variations
- Sperm and Testicular Function
- Neuroendocrine regulation and behavior
- Fish Ecology and Management Studies
- Muscle Physiology and Disorders
- Animal Behavior and Reproduction
- Physiological and biochemical adaptations
- Fluid Dynamics and Turbulent Flows
- Energy, Environment, Economic Growth
- Reproductive Biology and Fertility
- CRISPR and Genetic Engineering
- Plasma and Flow Control in Aerodynamics
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Amphibian and Reptile Biology
- interferon and immune responses
- Dermatologic Treatments and Research
- Genetic and Kidney Cyst Diseases
- Fluid Dynamics and Vibration Analysis
- Climate Change Policy and Economics
Southwest University
2020-2025
Zhongnan University of Economics and Law
2022
Chinese Academy of Fishery Sciences
2022
Southwest Medical University
2017
Institute of Hydrobiology
2014-2016
Chinese Academy of Sciences
2014-2016
Air Force Engineering University
2016
University of Chinese Academy of Sciences
2014-2015
Disruption of androgen signaling is known to cause testicular malformation and defective spermatogenesis in zebrafish. However, knockout
Prolactin (PRL) is an anterior pituitary hormone with a broad range of functions. Its ability to stimulate lactogenesis, maternal behavior, growth and development, osmoregulation, epithelial ion transport has been reported in many vertebrates. In our present study, we have targeted the zebrafish prl locus via transcription activator-like effector nucleases (TALENs). Two independent mutant lines premature termination putative sequence PRL peptides were generated. All prl-deficient progeny...
The sex determination for zebrafish is controlled by a combination of genetic and environmental factors. in has been suggested to rely on mechanism that affected germ cell-derived signals. To begin our current study, simplified efficient cell-specific promoter the dead end (dnd) gene was identified. Utilizing metrodinazole (MTZ)/ bacterial nitroreductase (NTR) system inducible cell ablation, several stable Tg (dnd:NTR-EGFP-3'UTR) (dnd:NTR-EGFP+3'UTR) lines were then generated with identified...
Intense stresses caused by high-altitude environments may result in noticeable genetic adaptions native species. Studies of adaptations to high elevations have been largely limited terrestrial animals. How fish adapt high-elevation is unknown. Triplophysa bleekeri, an endemic inhabiting regions, excellent model investigate the mechanisms adaptation local environment. Here, we assembled a chromosomal genome sequence T. with size ∼628 Mb (contig and scaffold N50 3.1 22.9 Mb, respectively). We...
Abstract Polyploidy, a significant catalyst for speciation and evolutionary processes in both plant animal kingdoms, has been recognized long time. However, the exact molecular mechanism that leads to polyploid formation, especially vertebrates, is not fully understood. Our study aimed elucidate this phenomenon using zebrafish model. We successfully achieved an effective knockout of cyclin N-terminal domain containing 1 (cntd1) CRISPR/Cas9 technology. This resulted impaired formation meiotic...
Chorionic gonadotropin α (Cgα) functions as the shared subunit for thyroid-stimulating hormone β (Tshβ), luteinizing (Lhβ), and follicle-stimulating (Fshβ). While these β-subunits have been extensively studied using effective gene knockout models in zebrafish, biological role of Cgα remains elusive. In this study, cgα-deficient zebrafish generated via transcription activator-like effector nucleases (TALENs) exhibited viability but displayed pronounced developmental abnormalities, including...
The zebrafish skeletal muscle-specific promoter mylz2 was used to cause crucian carp overexpression of the IGF-1 cDNA. In stable transgenic germline F1 progenies, a 5-fold increase in level muscle observed. Evident hyperplasia observed fish through histologic analysis. By analyzing RNA sequencing transcriptome and nontransgenic control at 15 months age, 10 966 transcripts with significant expression levels were identified definite gene descriptions based on corresponding genome information....
Sexual maturation and somatic growth cessation are associated with adolescent development, which is precisely controlled by interconnected neuroendocrine regulatory pathways in the endogenous endocrine system. The pituitary gland one of key regulators By analyzing RNA sequencing (RNA-seq) transcriptome before after sexual maturation, this study, we characterized global gene expression patterns zebrafish pituitaries at 45 90 days post-fertilization (dpf). A total 15 043 annotated genes were...
The regularity of Piwi-interacting RNA (piRNA) biogenesis is crucial to germline development. Functioning as proteins, Tudor domain-related proteins (Tdrds) have been demonstrated be involved in spermatogenesis and the piRNA pathway. In this study, zebrafish tdrd12 was identified, maternal germ cell-specific expression patterns were observed. Utilizing TALEN (transcription activator-like effector nuclease) techniques, two independent mutant lines generated. Although no defects found during...
Abstract It has been suggested that many novel RNA-binding proteins (RBPs) are required for gametogenesis, but the necessity of few these functionally verified. Here, we identified one RBP, Rbm46, and investigated its expression pattern role in zebrafish reproduction. We found rbm46 is maternally provided specifically expressed germ cells gonadal tissues using situ hybridization, reverse transcription-PCR, quantitative real-time polymerase chain reaction (qRT-PCR). Two independent mutant...
Sexually dimorphic mating behaviors differ between sexes and involve gonadal hormones possibly sexually gene expression in the brain. However, associations among brain, gonad, sexual behavior teleosts are still unclear. Here, we utilized germ cells-free tdrd12 knockout (KO) zebrafish, steroid synthesis enzyme cyp17a1-deficient zebrafish to investigate differences interplays brain-gonad-behavior axis, molecular control of brain dimorphism male behaviors.
Abstract The presence of breeding tubercles (BTs) on the pectoral fins has been investigated as a typical male secondary sexual characteristic (SSC) that distinguish males from females in adult zebrafish. Nonetheless, earliest occurrence these and its association with puberty onset body growth remain unclear. In this study, using morphological, histological statistical analyses, authors examined first appearance BTs zebrafish, particular emphasis potential impact them. results study revealed...
Fish skin is mainly composed of the epidermis, dermis, and its derivative scales. There a wide diversity in scale number fishes, but structure lacks systematic histological comparison. This research aimed to improve our understanding functional relationship between freshwater fishes determine which ecological factors affect structure. First, we presented method quantify fish histologically quantified 54 fishes. Second, collected habitat information 509 Cyprinidae China explored were related...
In light of China's implementation the Environmental Protection Tax Law (EPT Law) on 1 January 2018, this paper empirically investigates influence environmental regulations investment behaviour heavily polluting firms using a difference-in-differences (DID) model. The EPT is found to improve efficiency by restraining overinvestment, an effect that applies mainly private firms, particularly those with no political affiliations. This enriches existing research economic consequences integrating...
Abstract Background: Sexually dimorphic mating behaviors differ between sexes and involve gonadal hormones possibly sexually gene expression in the brain. However, associations among brain, gonad, sexual behavior teleosts are still unclear. Here, we utilized germ cells-free tdrd12 knockout (KO) zebrafish, steroid synthesis enzyme cyp17a1- deficient zebrafish to investigate differences interactions brain-gonad-behavior axis, molecular control of brain dimorphism male behaviors. Methods:...
Objective To observe the therapeutic effect of LS-200 semiconductor laser for subcutaneous hematoma and induration. Methods A randomized controlled trial was performed on 200 patients with induration. The were divided into an experimental group a control group, 100 each group. treated therapy, took no special treatment, 3 treatment courses (5 days 1 course). data statistically processed using SPSS 22.0 statistical software. Results The effective rate 94% in 70%...