A5110785460- Cancer-related molecular mechanisms research
- Adipose Tissue and Metabolism
- Reproductive biology and impacts on aquatic species
- Connexins and lens biology
- RNA modifications and cancer
- Heat shock proteins research
- interferon and immune responses
- Pharmacological Effects of Natural Compounds
- Animal Genetics and Reproduction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Herpesvirus Infections and Treatments
- Sperm and Testicular Function
- Epigenetics and DNA Methylation
- Advanced Biosensing Techniques and Applications
- DNA Repair Mechanisms
- Retinal and Optic Conditions
- Histone Deacetylase Inhibitors Research
- Muscle Physiology and Disorders
- Environmental Monitoring and Data Management
- Gene Regulatory Network Analysis
- Nuclear Receptors and Signaling
- Advanced Electron Microscopy Techniques and Applications
- Autophagy in Disease and Therapy
- Genetics, Aging, and Longevity in Model Organisms
- Regulation of Appetite and Obesity
Xiangyang Hospital of Traditional Chinese Medicine
2024-2025
China Agricultural University
2022-2024
Zhengzhou University
2021-2024
Institute of Hydrobiology
2014-2019
Chinese Academy of Sciences
2014-2019
University of Chinese Academy of Sciences
2014-2019
Highlights•Zebrafish Cyp2r1 is a major but not exclusive contributor to 25(OH)D in vivo•Cyp2r1-deficient zebrafish exhibit decreased somatic growth and obesity•Vitamin D signaling promotes fatty acid oxidation by inducing Pgc1a expressionSummary1α,25(OH)2D3 (vitamin D3) crucial for mineral homeostasis mammals, the precise effects of 1α,25(OH)2D3 adipose tissue remain be clarified vivo. The initial 25-hydroxylation catalyzed liver microsomal cytochrome P450 2R1 (CYP2R1), which conserved...
Cytochrome P450 (Cyp)17A1 has both 17α-hydroxylase and 17,20-lyase activities, which are involved in the steroidogenic pathway that produces androgens estrogens. Previously, a phenotype of all-male cyp17a1-deficient zebrafish generated by transcription activatorlike effector nuclease been reported. In current study, mechanisms relating to Cyp17a1 development sexual traits, especially gonadal differentiation testicular development, were characterized. We found fish at 3 months...
Insulin, the most potent anabolic hormone, is critical for somatic growth and metabolism in vertebrates. Type 2 diabetes, which primary cause of hyperglycemia, results from an inability insulin to signal glycolysis gluconeogenesis. Our previous study showed that double knockout receptor a ( insra) b insrb) caused β-cell hyperplasia lethality 5 16 days postfertilization (dpf) (Yang BY, Zhai G, Gong YL, Su JZ, Han D, Yin Z, Xie SQ. Sci Bull (Beijing) 62: 486-492, 2017). In this study, we...
SARS-Cov-2 infection, which has caused the COVID-19 global pandemic, triggers cellular senescence. In this study, we investigate role of SARS-COV-2 spike protein (S-protein) in regulating senescence RPE cells. The results showed that administration or overexpression S-protein ARPE-19 decreased cell proliferation with cycle arrest at G1 phase. increased SA-β-Gal positive cells high expression P53 and P21, senescence-associated inflammatory factors (e.g., IL-1β, IL-6, IL-8, ICAM, VEGF), ROS....
In recent studies, luteinizing hormone (LH) was reported to play important roles in oocyte maturation. However, the mechanism by which LH signaling, especially regarding steroidogenesis process, affects maturation has not been clarified. this study, zebrafish models with a functional deficiency beta (Lhb) or steroidogenic acute regulatory protein (Star), an enzyme that promotes transport of cholesterol into inner mitochondrial membrane for maturation-induced (MIH) production, were generated...
Abstract The endocrine regulatory roles of the hypothalamic–pituitary–adrenocortical axis on anxiety-like behavior and metabolic status have been found throughout animal taxa. However, precise effects balancing adrenal corticosteroid biosynthesis under influence adrenocorticotrophic hormone (ACTH), a pro-opiomelanocortin (POMC)-derived peptide, energy expenditure somatic growth remain unknown. POMC has also identified as one candidate loci for polycystic ovary syndrome, which features...
Aging | doi:10.18632/aging.203496. Dan-Dan Chen, Xuyan Peng, Yuxuan Wang, Mingjun Jiang, Mengjiao Xue, Guohui Shang, Xuhui Liu, Xiaolin Jia, Baixue Yingwei Lu, Hongmei Mu, Fengyan Zhang, Yanzhong Hu
Human AlkB homolog 6, ALKBH6, plays key roles in nucleic acid damage repair and tumor therapy. However, no precise structural functional information are available for this protein. In study, we determined atomic resolution crystal structures of human holo-ALKBH6 its complex with ligands. members bind acids by NRLs (nucleotide recognition lids, also called Flips), which can recognize DNA/RNA flip methylated lesions. We found that ALKBH6 has unusual Flip1 Flip2 domains, distinct from other...
Chorionic gonadotropin α (Cgα) functions as the shared subunit for thyroid-stimulating hormone β (Tshβ), luteinizing (Lhβ), and follicle-stimulating (Fshβ). While these β-subunits have been extensively studied using effective gene knockout models in zebrafish, biological role of Cgα remains elusive. In this study, cgα-deficient zebrafish generated via transcription activator-like effector nucleases (TALENs) exhibited viability but displayed pronounced developmental abnormalities, including...
Comprehensive Summary Aromatic polyketides (APKs) are renowned for their structural diversity and potent biological activities, making them valuable resources drug discovery in antibiotics, anticancer agents, antiparasitic therapies. Bipentaromycins, a unique class of dimeric APKs with cyclic head‐to‐tail linkage, exhibit broad‐spectrum antibacterial activities. Acylation modifications bipentaromycins C–F enhance pharmacological properties, yet the responsible acyltransferase remains...
The zebrafish skeletal muscle-specific promoter mylz2 was used to cause crucian carp overexpression of the IGF-1 cDNA. In stable transgenic germline F1 progenies, a 5-fold increase in level muscle observed. Evident hyperplasia observed fish through histologic analysis. By analyzing RNA sequencing transcriptome and nontransgenic control at 15 months age, 10 966 transcripts with significant expression levels were identified definite gene descriptions based on corresponding genome information....
Epigenetic modulations lead to changes in gene expression, including DNA methylation, histone modifications, and noncoding RNAs. In recent years, epigenetic modifications have been related the pathogenesis of different types cancer, cardiovascular disease, other diseases. Emerging evidence indicates that methylation could be associated with ischemic stroke (IS) plays a role pathological progression, but underlying mechanism has not yet fully understood. this study, we used human 850K...
Adenine N6 methylation in DNA (6mA) is a well-known epigenetic modification bacteria, phages, and eukaryotes. Recent research has identified the Mpr1/Pad1 N-terminal (MPN) domain-containing protein (MPND) as sensor that may recognize 6mA However, structural details of MPND molecular mechanism their interaction remain unknown. Herein, we report first crystal structures apo-MPND MPND-DNA complex at resolutions 2.06 Å 2.47 Å, respectively. In solution, assemblies both are dynamic. addition, was...
N6-methyladenine (6mA) of DNA is an emerging epigenetic mark in the genomes Chlamydomonas, Caenorhabditis elegans, and mammals recently. Levels 6mA undergo drastic fluctuation thus affect fertility during meiosis early embryogenesis. Here, we showed three complex structures demethylase C. elegans NMAD-1A, a canonical isoform NMAD-1 (F09F7.7). Biochemical results revealed that NMAD-1A prefers Bubble or Bulge DNAs. Structural studies unexpected "stretch-out" conformation its Flip2 region,...
Abstract Testicular nuclear receptor 4 (TR4) modulates the transcriptional activation of genes and plays important roles in many diseases. The regulation TR4 on target involves direct interactions with DNA molecules via DNA-binding domain (DBD) recruitment coregulators by ligand-binding (LBD). However, their regulatory mechanisms are unclear. Here, we report high-resolution crystal structures TR4DBD, TR4DBD–DNA complexes TR4LBD–JAZF1 complex. For recognition, multiple factors come into play,...
Abstract The dysfunction of CRALBP, a key regulator the visual cycle, is associated with retinitis punctata albescens characterized by night vision loss and retinal degeneration. In this paper, we find that expression CRALBP regulated heat shock protein 90 (HSP90). Inhibition HSP90α or HSP90β using CRISPR‐Cas9 technology downregulates CRALBP's mRNA in ARPE‐19 cells triggering degradation transcription factor SP1 ubiquitin–proteasome pathway. can bind to promoter, inhibition its inhibitor...
Genetic mutations in retinol dehydrogenase 5 (RDH5), a rate-limiting enzyme of the visual cycle, is associated with nyctalopia, AMD and stationary congenital fundus albipunctatus (FA). A majority these impair RDH5 protein expression intracellular localization. However, regulatory mechanisms underlying metabolism remain unclear. Here, we find that undergoes degradation via autophagy-lysosomal pathway, its stability regulated by interacting HSP90. Deletion HSP90α or HSP90β CRISPR-Cas9...
The involvement of long non-coding RNA (lncRNA) in bone marrow mesenchymal stem cell (MSC) osteogenic differentiation during osteoporosis (OP) development has attracted much attention. In this study, we aimed to disclose how LINC01089 functions human (hMSC) differentiation, and study the mechanism by which regulates MSC osteogenesis.