A5008328392- 3D Printing in Biomedical Research
- Bone Tissue Engineering Materials
- Mesenchymal stem cell research
- Orthopaedic implants and arthroplasty
- Electrospun Nanofibers in Biomedical Applications
- Graphene and Nanomaterials Applications
- Cellular Mechanics and Interactions
- Fullerene Chemistry and Applications
- Graphene research and applications
- dental development and anomalies
- Advancements in Battery Materials
- Synthesis and Characterization of Heterocyclic Compounds
- RNA Research and Splicing
- Oral and Maxillofacial Pathology
- Machine Learning in Materials Science
- Plant and Fungal Species Descriptions
- Extracellular vesicles in disease
- Wound Healing and Treatments
- Bone and Dental Protein Studies
- Nuclear materials and radiation effects
- Multiple Myeloma Research and Treatments
- Periodontal Regeneration and Treatments
- Electromagnetic Fields and Biological Effects
- Plant Pathogens and Resistance
- Cancer-related molecular mechanisms research
University of Bergen
2021-2025
Malmö University
2019
King's College London
2019
Kyoto University
2012
University of Tsukuba
2002-2011
There is growing evidence that extracellular vesicles (EVs) play a crucial role in the paracrine mechanisms of transplanted human mesenchymal stem cells (hMSCs). Little known, however, about influence microenvironmental stimuli on osteogenic effects EVs. This study aimed to investigate properties and functions EVs derived from undifferentiated hMSC (Naïve-EVs) during early stage osteogenesis (Osteo-EVs). A further aim was assess osteoinductive potential Osteo-EVs for bone regeneration rat...
Abstract Objective We aimed to develop a nanoemulgel of stem cells from human exfoliated deciduous teeth–derived conditioned medium (SHED-CM) for oral wound biotherapy candidate. Materials and Methods Deciduous tooth pulp was collected two patients aged 6 years. The mesenchymal cell marker expression analyzed by immunocytochemistry CD45, CD90, CD105. Alizarin red staining performed differentiate SHEDs osteoblasts. quantitative quantification transforming growth factor-β (TGF-β) vascular...
K2NiF4-type LaSrAlO4 and Sr2TiO4 exhibit anisotropic isotropic thermal expansion, respectively; however, their structural origin is unknown. To address this unresolved issue, the crystal structure expansion of have been investigated through high-temperature neutron synchrotron X-ray powder diffraction experiments ab initio electronic calculations. The coefficient (TEC) along c-axis (αc) being higher than that a-axis (αa) [αc = 1.882(4)αa] mainly ascribed to TEC interatomic distance between...
The fatal determination of bone marrow mesenchymal stem/stromal cells (BMSC) is closely associated with mechano-environmental factors in addition to biochemical clues. aim this study was induce osteogenesis the absence chemical stimuli using a custom-designed laminar flow bioreactor. BMSC were seeded onto synthetic microporous scaffolds and subjected subphysiological level fluid for up 21 days. During perfusion, cell proliferation significantly inhibited. There also morphological changes,...
Various perfusion bioreactor systems have been designed to improve cell culture with three-dimensional porous scaffolds, and there is some evidence that fluid force improves the osteogenic commitment of progenitors. However, because unique design concept operational configuration each study, experimental setups are not always compatible other systems. To reconcile results from different systems, thorough optimization validation required in system. In this optimal conditions for a were...
Abstract The fate determination of bone marrow mesenchymal stem/stromal cells (BMSC) is tightly regulated by mechanical cues, including fluid shear stress. Knowledge mechanobiology in 2D culture has allowed researchers tissue engineering to develop 3D dynamic systems with the potential for clinical translation which and growth BMSC are mechanically controlled. However, due complexity cell compared counterpart, mechanisms regulation environment remain relatively undescribed. In present study,...
Abstract Bioprinting allows for the fabrication of tissue-like constructs by precise architecture and positioning bioactive hydrogels with living cells. This study was performed to determine effect very low concentrations alginate (0.1, 0.3, 0.5% w/v) on bioprinting bone marrow mesenchymal stem cells (BMSC) in gelatin methacryloyl (GelMA; 5% w/v)/alginate blend. Furthermore, while GelMA photocrosslinked all bioprinted constructs, crosslinking calcium chloride physical biological...
The diversity of cyanobacterial species within the coralloid roots an individual and populations Cycas revoluta was investigated based on 16S rRNA gene sequences. Sixty-six were collected from nine natural cycads Kyushu Ryukyu Islands, covering entire distribution range species. Approximately 400 bp 5'-end genes amplified, each identified by denaturing gradient gel electrophoresis. Most harbored only one cyanobiont, Nostoc, whereas some contained two or three, representing cyanobiont a...
The structural phase transition of Pr(1-x)Ca(x)MnO(3)(x = 3/8) was investigated by means low-temperature transmission electron microscopy. Superlattice reflection spots with a modulation wave vector q(1) (0,1/2,0) appeared below 230 K, indicating formation the d(3x(2-r(2))/d(3y(2)-r(2)) type charge/orbital ordering. Below 150 new series superlattice q(2) (1/4,1/4,1/2) appeared, suggesting an additional ordering excess 1/8 Mn(3+), necessary due to deviation x from 1/2, occupation d(3z(2-r(2))...
This study investigated the level of magnetic energy around implants possessing a static field (SMF) and assessed in vivo influence SMF on bone regeneration. Implants sintered neodymium magnet internally were placed rabbit femur. An implant without was as control. After 12 weeks healing vivo, samples subjected to histologic/histomorphometric evaluation. The bone-to-implant contact for test group control 32.4 ± 13.6% 17.1 4.5%, respectively, differences statistically significant (P < .05)....
Tooth germs undergo a series of dynamic morphologic changes through bud, cap, and bell stages, in which odontogenic epithelium continuously extends into the underlying mesenchyme. During transition from bud stage to cap stage, base flattens then bends shape whose edges are referred as “cervical loops.” Although genetic mechanisms for formation have been well described, little is understood about morphogenetic mechanisms. Computer modeling cell trajectory tracking suggested that epithelial...
Abstract Various types of synthetic polyesters have been developed as biomaterials for tissue engineering. These materials commonly possess biodegradability, biocompatibility, and formability, which are preferable properties bone regeneration. The major challenge using is the result low cell affinity due to their hydrophobic nature, hinders efficient seeding active dynamics. To improve wettability, plasma treatment widely used in industry. Here, we performed surface activation with oxygen...
Three-dimensional printing (3D printing) is a promising technique for producing scaffolds bone tissue engineering applications. Porous can be printed directly, and the design, shape porosity controlled. 3D synthetic biodegradable polymeric intended in situ regeneration must meet stringent criteria, primarily appropriate mechanical properties, good adequate biocompatibility ability to enhance formation. In this study, healing of critical-sized (5 mm) femur defects rats was enhanced by...
Degradable polyester-based scaffolds are ideal for tissue engineering applications where long-term structural integrity and mechanical support a requisite. However, their hydrophobic unfunctionalized surfaces restrain tissue-mimetic quality. Instead, hyaluronan (HA) hydrogels able to act as cell-instructive materials with the ability recapitulate native tissue, although HA is rapidly metabolized in vivo. Taking advantage of these distinctly diverse material properties, degradable concurrent...
Osteocytes perceive and process mechanical stimuli in the lacuno-canalicular network bone. As a result, they secrete signaling molecules that mediate bone formation resorption. To date, few three-dimensional (3D) models exist to study response of mature osteocytes biophysical mimic fluid shear stress substrate strain mineralized, biomimetic bone-like environment. Here we established 3D model by utilizing state-of-art perfusion bioreactor platform where immortomouse/Dmp1-GFP-derived...
The structural and electronic modifications of C 60 monolayer on Cu(111) Pt(111) have been studied by scanning tunneling microscopy. On Cu(111), controlled experiments demonstrated that the structure layer changes markedly with increasing extent interaction between substrate. most strongly interacting exhibited a metallic density states at Fermi energy. Pt(111), where substrate is stronger, annealing caused decomposition layer, resulting in graphene formation instead polymerization.
Understanding cellular mechanics requires evaluating the mechanical and chemical cues that regulate actin cytoskeleton, particularly filopodia lamellipodia. Therefore, this study aims to investigate effect of scaffolds properties on cell migration. The results showed toughness, strain, strength played a key role in promoting movement by stimulating dynamic formation test sample containing 3 wt% G significantly enhanced yield strength, leading increase motility as well development longer...
The structural and electronic modifications of C60 monolayer on Cu(111) Pt(111) have been studied by scanning tunneling microscopy. On Cu(111), controlled experiments demonstrated that the structure layer changes markedly with increasing extent interaction between substrate. most strongly interacting exhibited a metallic density states at Fermi energy. Pt(111), where substrate is stronger, annealing caused decomposition layer, resulting in graphene formation instead polymerization.
Abstract Background IRX3 is implicated in genetic predisposition to obesity via the FTO variant locus. shows risk allele-dependent upregulation specifically during early adipogenesis, leading a shift from energy-dissipation fat storage mature adipocytes. However, how changes expression at one developmental stage affect cellular phenotype later remains unclear. We here hypothesize that regulates adipocyte development transcriptional modulation of epigenetic reprogramming factors. Methods...
Several synthetic polymers have been widely investigated for their use in bone tissue engineering applications, but the ideal material is yet to be engineered. Triazine-trione (TATO) based materials and derivatives are novel field of biomedical started draw interest. Different designs TATO monomers introduction different chemical linkages end-groups widens scope due a range mechanical properties. The aim our work investigate materials, with or without hydroxyapatite filler, potential...