A5050162741- Bone Tissue Engineering Materials
- Periodontal Regeneration and Treatments
- Bone and Dental Protein Studies
- Mesenchymal stem cell research
- Orthopaedic implants and arthroplasty
- Dental Implant Techniques and Outcomes
- Bone fractures and treatments
- Bone Metabolism and Diseases
- Bone health and treatments
- Anatomy and Medical Technology
- Titanium Alloys Microstructure and Properties
- Connective tissue disorders research
- Pharmacological Effects of Medicinal Plants
- Tissue Engineering and Regenerative Medicine
- MicroRNA in disease regulation
- Anesthesia and Neurotoxicity Research
- 3D Printing in Biomedical Research
- Organ Transplantation Techniques and Outcomes
- Circular RNAs in diseases
- Bone and Joint Diseases
- Dental Trauma and Treatments
- Innovations in Concrete and Construction Materials
- Oral and gingival health research
- Facial Trauma and Fracture Management
- Forensic Anthropology and Bioarchaeology Studies
Ludwig Boltzmann Institute for Experimental and Clinical Traumatology
2021-2025
Ludwig Boltzmann Institute for Digital Health and Prevention
2021-2025
Medical University of Vienna
2021-2025
Austrian Cluster for Tissue Regeneration
2021-2025
University of Vienna
2018-2024
Human mesenchymal stromal cells (hMSCs) seeded on calcium phosphate (CaP) bioceramics are extensively explored in bone tissue engineering and have recently shown effective clinical outcomes. In previous pre-clinical studies, hMSCs-CaP-mediated formation was preceded by osteoclastogenesis at the implantation site. The current study evaluates to what extent phase composition of CaPs affects osteoclast response ultimately influence formation. To this end, four different CaP were used,...
There is growing evidence that extracellular vesicles (EVs) play a crucial role in the paracrine mechanisms of transplanted human mesenchymal stem cells (hMSCs). Little known, however, about influence microenvironmental stimuli on osteogenic effects EVs. This study aimed to investigate properties and functions EVs derived from undifferentiated hMSC (Naïve-EVs) during early stage osteogenesis (Osteo-EVs). A further aim was assess osteoinductive potential Osteo-EVs for bone regeneration rat...
Abstract Although autografts are considered to be the gold standard treatment for reconstruction of large bone defects resulting from trauma or diseases, donor site morbidity and limited availability restrict their use. Successful repair also depends on sufficient vascularization address this challenge, novel strategies focus development vascularized biomaterial scaffolds. This pilot study aimed investigate feasibility regenerating in sheep using 3D-printed customized calcium phosphate...
Abstract Background Three-dimensional (3D) spheroid culture can promote the osteogenic differentiation of bone marrow mesenchymal stromal cells (BMSC). 3D printing offers possibility to produce customized scaffolds for complex defects. The aim this study was compare potential human BMSC cultured as 2D monolayers or spheroids encapsulated in constructs 3D-printed poly-L-lactide-co-trimethylene carbonate and modified platelet lysate hydrogels (PLATMC-HPLG) regeneration. Methods PLATMC-HPLG...
Functionalizing biomaterials with conditioned media (CM) from mesenchymal stromal cells (MSC) is a promising strategy for enhancing the outcomes of guided bone regeneration (GBR). This study aimed to evaluate regenerative potential collagen membranes (MEM) functionalized CM human marrow MSC (MEM-CM) in critical size rat calvarial defects. MEM-CM prepared via soaking (CM-SOAK) or followed by lyophilization (CM-LYO) were applied Control treatments included native MEM, MEM (CEL) and no...
Bio-Gide® is a collagen membrane routinely used in guided bone regeneration. Recent studies have shown that this has osteoconductive properties, meaning it can support the growth of new bone. However, also been observed areas mineralized fibers which occur spontaneously and independently osteoblasts. To better understand how works, we established model using minced membranes to reduce active mineralization intact favor passive mineralization. We thus compared original with 5 mm calvarial...
Bone healing is a well-orchestrated process involving various bone cells and signaling pathways, where disruptions can result in delayed or incomplete healing. MicroRNAs (miRNAs) are small non-coding RNAs capable of influencing cellular processes, including remodeling. Due to their biological relevance stable presence biofluids, miRNAs may serve as candidates for diagnosis prognosis The aim the study was investigate changes circulating blood during rat calvaria defects biomarkers successful...
Platelet-rich fibrin, the coagulated plasma fraction of blood, is commonly used to support natural healing in clinical applications. The rat calvaria defect a standardized model study bone regeneration. It remains, however, unclear if appropriate investigate impact human PRF (Platelet-Rich Fibrin) on To this end, we soaked Bio-Gide® collagen membranes or liquid concentrated before placing them onto 5 mm calvarial defects Sprague Dawley rats. Three weeks later, histology and micro-computed...
Three-dimensional (3D) spheroid culture can promote the osteogenic differentiation and bone regeneration capacity of mesenchymal stromal cells (MSC). Gingiva-derived progenitor (GPC) represent a less invasive alternative to marrow MSC (BMSC) for clinical applications. The aim this study was test in vivo forming potential human GPC BMSC cultured as 3D spheroids or dissociated (2D). 2D encapsulated constructs platelet lysate hydrogels (HPLG) 3D-printed poly (L-lactide-co-trimethylene...
A major challenge in orthopedics is the repair of large non-union bone fractures.
Autogenous tooth roots are increasingly applied as a grafting material in alveolar bone augmentation. Since undergo creeping substitution similar to grafts, it can be hypothesized that osteoclasts release the growth factors stored dentin thereby influencing formation. To test this hypothesis, collagen membranes were either soaked acid lysates (ADL) from extracted porcine teeth or serum-free medium followed by lyophilization. Thereafter, these covered standardized 5-mm-diameter critical-size...
A static magnetic field generated by neodymium–iron–boron (NdFeB) magnets placed in the inner cavity of dental implants can enhance bone regeneration rabbits. It is, however, unknown whether fields support osseointegration a canine model. We therefore determined potential osteogenic effect carrying NdFeB inserted tibia six adult canines early stages osseointegration. Here, we report that after 15 days healing, and regular showed high variation with median new bone-to-implant contact (nBIC)...
Abstract Objectives Secretomes of mesenchymal stromal cells (MSC) represent a novel strategy for growth‐factor delivery tissue regeneration. The objective this study was to compare the efficacy adjunctive use conditioned media bone‐marrow MSC (MSC‐CM) with collagen barrier membranes vs. leukocyte‐ and platelet‐rich fibrin (PRF‐CM), current therapy, guided bone regeneration (GBR). Methods MSC‐CM PRF‐CM prepared from healthy human donors were subjected proteomic analysis using mass...
Large non-union bone fractures are a significant challenge in orthopedic surgery. Although auto- and allogeneic grafts excellent for healing such lesions, there potential complications with their use. Thus, material scientists developing synthetic, biocompatible biomaterials to overcome these problems. In this study, we present multidisciplinary platform evaluating repair. We combined expertise from biology immunology develop including vitro osteoclast (OC) osteoblast (OB) assays vivo mouse...
Abstract Objectives Chronic liver disease increases the risk for periodontal and osteoporotic fractures, but its impacts on bone regeneration remain unknown. Herein, we studied impact of cirrhosis peri‐implant formation. Material Methods A total 20 male Wistar rats were randomly divided into two groups: one with common bile duct ligated (BDL) respective sham‐treated control group (SHAM). After four weeks induction, titanium mini‐screws inserted tibia. Successful induction was confirmed by...
Searchable abstracts of presentations at key conferences on calcified tissues ISSN 2052-1219 (online)
Searchable abstracts of presentations at key conferences on calcified tissues ISSN 2052-1219 (online)
Abstract Background Secretomes of mesenchymal stromal cells (MSC) are emerging as a novel strategy for growth factor delivery and promising alternative to cell therapies tissue regeneration. The objective this study was compare the efficacy adjunctive use conditioned media human MSC (MSC-CM) with collagen barrier membranes (MEM) guided bone regeneration (GBR) vs. leukocyte- platelet-rich fibrin (PRF-CM). Methods MSC-CM PRF-CM were prepared from three donors each subjected quantitative...