A5086028468- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Cancer Immunotherapy and Biomarkers
- T-cell and B-cell Immunology
- Genetics, Bioinformatics, and Biomedical Research
- Cervical Cancer and HPV Research
- Neuroblastoma Research and Treatments
- Biotechnology and Related Fields
- Machine Learning in Bioinformatics
- COVID-19 Clinical Research Studies
- Cytokine Signaling Pathways and Interactions
- Childhood Cancer Survivors' Quality of Life
- Viral gastroenteritis research and epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Pediatric Urology and Nephrology Studies
- vaccines and immunoinformatics approaches
- Acute Lymphoblastic Leukemia research
- SARS-CoV-2 and COVID-19 Research
- Heat shock proteins research
- Hepatitis B Virus Studies
- Pancreatic function and diabetes
- Diet, Metabolism, and Disease
- Neonatal Health and Biochemistry
Moffitt Cancer Center
2021-2025
Universidade de Ribeirão Preto
2023
Universidade de São Paulo
2010-2023
Fundação Hemopa
2023
Instituto do Câncer do Estado de São Paulo
2020
Hospital São Paulo
2019
Abstract Background mRNAs are highly versatile, non-toxic molecules that easy to produce and store, which can allow transient protein expression in all cell types. The safety aspects of mRNA-based treatments gene therapy make this molecule one the most promising active components therapeutic or prophylactic methods. use mRNA as strategy for stimulation immune system has been used mainly current strategies cancer treatment but until now no tested vaccine infectious disease. Results We...
Background Adoptive transfer of tumor-infiltrating lymphocytes (TIL) is now a Food and Drug Administration (FDA)-approved treatment for melanoma. While this major milestone, there room improvement to increase clinical response rates further optimize the manufacturing TIL products. In study, we characterized association B-cells (TIL-B) tertiary lymphoid structures (TLSs) with therapy tested whether presence in tumor can be leveraged manufacture. Methods Tumor sections from responders (R, n=9)...
In DNA vaccines, the gene of interest is cloned into a bacterial plasmid that engineered to induce protein production for long periods in eukaryotic cells. Previous research has shown intramuscular immunization BALB/c mice with naked fragment encoding Mycobacterium leprae 65-kDa heat-shock (pcDNA3-Hsp65) induces protection against M. tuberculosis challenge. A key stage protective immune response after generation memory T Previously, we have B cells capture DNA-Hsp65 and thereby modulate...
Cervical cancer, which main etiologic factor is Human Papillomavirus (HPV) infection, continues to be a burden for public health systems in developing countries. Our laboratory has been working with the hypothesis that signals generated tumor microenvironment can modulate local and systemic immune responses. In this context, it would reasonable think tumors create pro-tumoral bias cells, even before they are recruited microenvironment. To understand if how signaling started influence cells...
The role of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) neutralizing antibody response from natural infection and vaccination, potential determinants this are poorly understood. Characterizing factors associated with neutralization can help inform future prevention efforts improve clinical outcomes in those infected.The goals study were to prospectively evaluate SARS-CoV-2 levels responses among naturally infected adults determine demographic behavioral independently...
Despite the remarkable success in use of T cells expressing Chimeric Antigen Receptors (CAR) for hematologic malignances, their application to solid tumors, which represents majority neoplasms, remains challenging. However, lymphocytes artificial Cell (TCR) with enhanced affinity, refered as TCR-T cells, have been providing encouraging results against neoplasms. Unlike conventional CAR, these receptors are able recognize intracellular proteins presented by Human Leukocyte (HLA), expanding...
The treatment of solid tumors with CAR-T cells is challenging due to the complex tumor microenvironment that impairs infiltration and persistence injected cells. Thus, enhancing resistance exhaustion desirable maximize therapeutic efficacy. GITR, a T cell costimulatory molecule, plays crucial role in modulating activation function. When engaged by its ligand GITRL, GITR signaling promotes proliferation, cytokine production, survival, leading enhanced effector functions. Additionally, can...
Adoptive therapy using Chimeric Antigen Receptor T-cell (CAR-T) technology has emerged as a groundbreaking immunotherapeutic approach for treating various hematological malignancies. Among the diverse CAR-T targets, CD19, B-cell surface marker, demonstrated remarkable success in However, despite impressive clinical outcomes, challenges such efficacy and insufficient vivo persistence still exist. To address these limitations, preclinical investigations are exploring integration of additional...
Introduction The use of myeloablative conditioning regimen followed by autologous hematopoietic stem cell infusion is considered as standard care for the first or second line treatment diverse types lymphoma. However, studies comparing different regimens are not frequent, and its choice can be based on transplant center's experience. In last few years, our center had choices due to unavailability some chemotherapy drugs, main options were BEAM, CBV busulfan (BuMel). Objective Perform a...
<h3>Background</h3> We designed a novel bispecific T-cell engager (BiTE) targeting the somatostatin receptor (SSTR), which is overexpressed by well-differentiated neuroendocrine tumors (NETs). In our BiTE, single chain variable fragment (scFV) based anti-SSTR domain replaced 2 molecules of somatostatin-14 (SST14), hormone that physiologically activates SSTR. <h3>Methods</h3> The sequence SST14-scFV anti-CD3 BiTE was optimized and cloned into pAcGP67avector. recombinant protein expressed...
<h3>Background</h3> The immunogenic nature of melanoma has been exploited for the development adoptive transfer ex-vivo expanded tumor infiltrating lymphocytes (TIL). This cell therapy overall response rates around 50%. Multiple factors may determine quality TIL product including components microenvironment. B-cells are frequently found in metastasis, and display signs antigen experience. Recently, B-cell infiltration associated with improved clinical responses to immune checkpoint...