The demand for lithium-ion batteries (LIBs) has dramatically increased in recent years due to their application various electronic devices and electric vehicles (EVs). Great amount of LIB waste is generated, most which ends up landfills. wastes contain substantial amounts critical metals (such as Li, Co, Ni, Mn, Cu) can therefore serve valuable secondary sources these metals. Metal recovery from the black mass (shredded spent LIBs) be achieved via bioleaching, a microbiology-based technology...

Experiments were carried out to examine redox transformations of copper and chromium by acidophilic bacteria (Acidithiobacillus, Leptospirillum Acidiphilium), also iron (III) reduction Acidithiobacillus spp. under aerobic conditions. Reduction was found with all five species tested, grown aerobically on elemental sulfur. Cultures maintained at pH 1.0 for protracted periods displayed increasing propensity reduction, which observed in cell-free culture as well those containing bacteria. At....

Hydrogen can serve as an electron donor for chemolithotrophic acidophiles, especially in the deep terrestrial subsurface and geothermal ecosystems. Nevertheless, current knowledge of hydrogen utilization by mesophilic acidophiles is minimal. A multi-omics analysis was applied on Acidithiobacillus ferrooxidans growing hydrogen, a respiratory model proposed. In model, [NiFe] hydrogenases oxidize to two protons electrons. The electrons are used reduce membrane-soluble ubiquinone ubiquinol....

Over the past 100 years, extensive oxidation of As-bearing sulfide-rich tailings from abandoned Long Lake Gold Mine (Canada) has resulted in formation acid mine drainage (pH 2.0–3.9) containing high concentrations dissolved As (∼400 mg L−1), SO42-, Fe and other metals. Dissolved is predominantly present as As(III), with increased As(V) near surface. Pore-gas O2 depleted to < 1 vol% upper 30–80 cm profile. The primary sulfides, pyrite arsenopyrite, are highly oxidized portions tailings....

In recent years, the demand for lithium-ion batteries (LIBs) has been increasing rapidly. Conventional recycling strategies (based on pyro- and hydrometallurgy) are damaging environment more sustainable methods need to be developed. Bioleaching is a promising environmentally friendly approach that uses microorganisms solubilize metals. However, bioleaching-based technology not yet applied recover valuable metals from waste LIBs an industrial scale. A series of experiments was performed...

Abstract Cellular ATP content of Acidithiobacillus ferrooxidans cultures was determined with a bioluminescence assay in relation to batch growth and oxidation ferrous iron elemental sulfur. Inhibitory effects inorganic substrates products on luciferase were eliminated. Extracellular levels negligible. The sulfur-grown cells decreased anomalously due culture pH increase at the stationary phase. Although rates sulfur reached original levels, remained constant because gradual decrease cellular...

According to the literature, pyrite (FeS2) oxidation has been previously determined involve thiosulfate as first aqueous intermediate sulfur product, which is further oxidized sulfate. In present study, by Acidithiobacillus ferrooxidans was studied using electrochemical and metabolic approaches in an effort extend existing knowledge on mechanism. Due small surface area, reaction rate of a compact electrode form polycrystalline aggregate A. suspension very slow at spontaneously formed high...

Acid mine/rock drainage (AMD/ARD) is generated by the microbially-accelerated oxidative dissolution of sulfide minerals in working and abandoned mine sites, some natural environments. Iron-oxidizing microorganisms (IOM) regenerate oxidant Fe3+, while sulfur-oxidizing (SOM) contribute to AMD/ARD generating H2SO4 via oxidation elemental S reduced inorganic sulfur compounds. Bacterial archaeal diversity 34 samples sulfide-bearing waste rock recovered from three boreholes at Faro Mine Complex...

Ten strains of extremely acidophilic bacteria, isolated from different environments form a distinct monophyletic clade within the phylum Firmicutes. Comparison complete genomes proposed type confirm that they comprise two genera (proposed names Sulfoacidibacillus and Ferroacidibacillus), at least three species (Sulfoacidibacillus ferrooxidans, thermotolerans Ferroacidibacillus organovorans). The bacterial share some physiological traits, including catalysing dissimilatory oxidation reduction...

Deep in situ biomining (DISB) offers the potential for exploiting metal-rich ore bodies that are currently difficult or non-economic to access, with far less environmental impact than current deep opencast mining practices. DISB is being developed within EU Horizon 2020 project "BIOMOre", which targets a calcareous sedimentary copper ore, kupferschiefer, widespread subterranean rock stratum central and north-western Europe. Laboratory tests on two main copper-bearing fractions of sandstone...

The Strathcona Waste Water Treatment System (SWWTS; Sudbury, ON, Canada) has received mill tailings from Ni/Cu ore processing 1970 to present. Demonstration-scale, multi-layer cover systems were installed on selected deposition cells at the SWWTS. are comprised of an upper layer organic carbon-rich material, composed a biosolids fertilizer along with composted municipal food and yard waste, then desulfurized, fine-grained tailings. Organic carbon components used in these covers promote...

Red mud (RM) is a highly alkaline polymetallic waste generated via the Bayer process during alumina production. It contains metals that are critical for sustainable development of modern society. Due to shortage global resources many metals, efficient large-scale processing RM has been receiving increasing attention from both researchers and industry. This study investigated solubilization RM, together with dealkalization, sulfur (S0) oxidation catalyzed by moderately thermophilic bacterium...

Industrial waste is accumulating, while primary metal resources are depleting. Bioleaching has been shown to be a cost-effective and environmentally friendly approach recovery from waste, but improved designs needed for large-scale recycling. Metal components that manufactured by electrodeposition over mandrel can difficult recycle using conventional techniques due their complex geometry inner Ag coating. A sustainable biotechnology separating Cu electrodeposited presented. Two-step...

The major objective of the EU Horizon 2020 project “BioMOre” is technical realization indirect in situ leaching Kupferschiefer sandstone and black shale ore by a ferric iron lixiviant generated mixed culture autotrophic, acidophilic, iron-oxidizing bacteria archaea iron-generating bioreactor (FIGB). These organisms could colonize deeply buried geological formations even under anaerobic conditions as most are able to grow coupling reduction oxidation reduced sulfur compounds absence oxygen....