A5075554752- Pharmacogenetics and Drug Metabolism
- Neonatal Health and Biochemistry
- Pancreatic function and diabetes
- Chromium effects and bioremediation
- Drug Transport and Resistance Mechanisms
- Glutathione Transferases and Polymorphisms
- Glycosylation and Glycoproteins Research
- Redox biology and oxidative stress
- Carbohydrate Chemistry and Synthesis
- Genomics, phytochemicals, and oxidative stress
- Environmental Toxicology and Ecotoxicology
- Sulfur Compounds in Biology
- Cancer-related Molecular Pathways
- Heavy metals in environment
- Enzyme function and inhibition
- Metal complexes synthesis and properties
- Mercury impact and mitigation studies
- Diabetes and associated disorders
- Histone Deacetylase Inhibitors Research
- Protein Tyrosine Phosphatases
- Literature, Film, and Journalism Analysis
- Zebrafish Biomedical Research Applications
- Chemotherapy-induced organ toxicity mitigation
- Biochemical Analysis and Sensing Techniques
- Chemotherapy-induced cardiotoxicity and mitigation
Laboratoire Interdisciplinaire des Environnements Continentaux
2016-2022
Université de Lorraine
2012-2022
Centre National de la Recherche Scientifique
1994-2022
Institut de Pharmacologie Moléculaire et Cellulaire
2011
Laboratoire d'Étude des Microstructures et de Mécanique des Matériaux
2001-2007
University of Arkansas for Medical Sciences
1996-2006
Eastern Virginia Medical School
2006
University of Helsinki
2006
Brigham and Women's Hospital
1999-2001
Harvard University
2001
Sodium butyrate (NaBu), a potent histone deacetylase inhibitor, modulates the expression of large number genes. The purpose this study was to determine whether dietary agent could induce apoptosis in MCF-7 cells, breast cancer cell line that lacks caspase-3 activity, and identify mechanisms underlie NaBu toxicity these cells. Cell viability assessed by activity mitochondrial succinate dehydrogenase (MTT assay) revealed dose-dependent reduction cellular growth response treatment. Restoring...
Abstract Despite the consensus about importance of chemical speciation in controlling bioavailability and ecotoxicity trace elements, detailed studies during laboratory testing remain scarce, contributing to uncertainty when extrapolating findings real field situations risk assessment. We characterized ecotoxicological effects chromium (Cr III Cr VI ) International Organization for Standardization (ISO) medium algal testing. Total dissolved (< 0.22 μm) concentrations showed little...
A phosphine-coordinated gold(I) thiosugar complex was found to exert potent cytotoxic and antiproliferative effects through thioredoxin reductase inhibition in MCF-7 cells.
4-Azido-2-hydroxybenzoic acid (4-AzHBA), a novel photoactive benzoic derivative, has been synthesized and used as photoprobe to identify the phenol binding site of UDP-glucuronosyltransferases (UGTs). Analysis recombinant His-tag UGTs from 1A family for their ability glucuronidate p-nitrophenol (pNP) 4-methylumbelliferone (4-MU) revealed that UGT1A10 shows high activity toward phenols derivatives. Purified was photolabeled with 4-AzHBA, digested trypsin, analyzed by matrix-assisted laser...
CDC25 phosphatases control cell cycle progression by activating cyclin dependent kinases. The three isoforms encoding genes are submitted to alternative splicing events which generate at least two variants for CDC25A and five both CDC25B CDC25C. An over-expression of was reported in several types cancer, including breast is often associated with a poor prognosis. Nevertheless, most the previous studies did not address expression splice variants. Here, we evaluated spliced transcripts...
The hepatic UDP-glucuronosyltransferase UGT1*6 is actively involved in the glucuronidation of short and planar phenols humans. Based on irreversible inhibition enzyme chemical modification by 2,3-butanedione diethyl pyrocarbonate, roles His54 Arg52 were investigated oligonucleotide site-directed mutagenesis. These amino acids belong to a consensus sequence LX2-R52-G-H54-X3-V-L located conserved hydrophobic region variable amino-terminal domain UGT. was replaced alanine (mutant R52A), or...
The treatment of UDP‐glucuronosyltransferase UGT1*6 stably expressed in V79 cells with three car☐yl‐specific reagents, dicyclohexylcarbodiimide, 1‐ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide and N ‐ethyl‐5‐phenylisoxazolium‐3′‐sulfonate (Woodward's reagent K), resulted a fast, dose‐dependent decrease the 4‐methylumbelliferone glucuronidation. inactivation reactions followed pseudo‐first order kinetics. p K modified residue was close to 5.0. A partial protection against by Woodward's...
Abstract A novel fluorescent photoactive probe 7‐azido‐4‐methylcoumarin (AzMC) has been characterized for use in photoaffinity labeling of the substrate binding site human phenol sulfotransferase (SULT1A1 or P‐PST‐1). For experiments, SULT1A1 cDNA was expressed Escherichia coli as a fusion protein to maltose (MBP) and purified apparent homogeneity over an amylose column. The moiety removed by Factor Xa cleavage. Both MBSULT1A1 were efficiently photolabeled with AzMC. This concentration...
A wide array of drugs, xenobiotics, and endogenous compounds undergo detoxification by conjugation with glucuronic acid in the liver via action UDP-glucuronosyltransferases. The mechanism whereby glucuronides, generated this enzyme system lumen endoplasmic reticulum (ER), are exported to cytosol prior excretion is unknown. We examined process purified rat microsomes using a rapid filtration technique [(3)H]estradiol-17beta-d-glucuronide ([(3)H]E(2)17betaG) as model substrate. Time-dependent...
The histone deacetylase inhibitor sodium butyrate induces several gene products that modify cellular metabolism. Here, we investigated its ability to modulate glutathione-related detoxification enzymes in the breast cancer cell line MCF-7 and a derivative resistant vincristine (VCREMS). We found induced glutathione S-transferase glutathione-dependent peroxidase activities triggered depletion. Expression of MRP1, an ATP-dependent GS-X pump, was unmodified. Moreover, isobologram analysis...
Reactive oxygen species regulate protein functionality. Cell cycle CDC25 phosphatases are targets of such oxidative regulation in vitro. We sought to evaluate if a thioredoxin (trx)-dependent redox exists cancer cells. For that purpose, we used MCF7 and MDA-MB 231 breast cells, which express trx1 differentially, together with two trx/thioredoxin reductase (trxR) inhibitors, Auranofin Acrolein. could induce full trxR inhibition associated ROS production both cell lines. Acrolein provoke...
Previous studies have documented the presence of protein-mediated transport UDP-glucuronic acid (UDP-GlcUA) in rat liver endoplasmic reticulum (ER). To determine crucial amino acids membrane transporter and evaluate their function regulating glucuronidation reaction, we examined effect histidyl-specific irreversible inhibitors on uptake radiolabeled UDP-GlcUA ER. Inactivation (initial rate) was more pronounced with hydrophobic reagents [diethyl pyrocarbonate (DEPC), p-bromophenacyl bromide]...