A5082361562- Neuroscience of respiration and sleep
- Genetics and Neurodevelopmental Disorders
- Diagnosis and Treatment of Venous Diseases
- Peripheral Artery Disease Management
- Cardiac, Anesthesia and Surgical Outcomes
- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- Central Venous Catheters and Hemodialysis
- Neonatal Respiratory Health Research
- Epilepsy research and treatment
- Respiratory Support and Mechanisms
- Anesthesia and Neurotoxicity Research
- Aortic Disease and Treatment Approaches
- Venous Thromboembolism Diagnosis and Management
- Vascular Procedures and Complications
- Stroke Rehabilitation and Recovery
- Vascular Anomalies and Treatments
- Heart Rate Variability and Autonomic Control
- Aortic aneurysm repair treatments
- Anesthesia and Pain Management
- Neuroendocrine regulation and behavior
- Neonatal and fetal brain pathology
- Neuroscience and Neuropharmacology Research
- High Altitude and Hypoxia
- Diversity and Career in Medicine
University of Connecticut
2017-2025
Medical University of South Carolina
2025
University of New Orleans
2022
Sanford Research
2014
Cerebral blood flow is highly sensitive to changes in CO2/H+ where an increase causes vasodilation and increased flow. Tissue also functions as the main stimulus for breathing by activating chemosensitive neurons that control respiratory output. Considering CO2/H+-induced would accelerate removal of potentially counteract drive breathe, we hypothesize brain regions have adapted a means preventing vascular CO2/H+-reactivity. Here, show rat purinergic signaling, possibly through P2Y2/4...
Dravet syndrome (DS) is a form of epilepsy with high incidence sudden unexpected death in (SUDEP). Respiratory failure leading cause SUDEP, and DS patients’ frequently exhibit disordered breathing. Despite this, mechanisms underlying respiratory dysfunction are unknown. We found that mice expressing DS-associated Scn1a missense mutation (A1783V) conditionally inhibitory neurons (Slc32a1cre/+::Scn1aA1783V fl/+; defined as Scn1aΔE26) spontaneous seizures, die prematurely present phenotype...
Abstract Pitt–Hopkins syndrome is an autism spectrum disorder caused by autosomal dominant mutations in the human transcription factor 4 gene (TCF4). One pathobiological process murine Tcf4 mutation a cell autonomous reduction oligodendrocytes and myelination. In this study, we show that promyelinating compounds, clemastine, sobetirome Sob-AM2 are effective at restoring myelination defects mouse model. vitro, clemastine treatment reduced excess oligodendrocyte precursor cells normalized...
Respiratory chemoreceptors regulate breathing in response to changes tissue CO 2 /H + . Blood flow is a fundamental determinant of , yet little known regarding how regulation vascular tone chemoreceptor regions contributes respiratory behavior. Previously, we showed rat that -vasoconstriction the retrotrapezoid nucleus (RTN) supports chemoreception by purinergic-dependent mechanism (Hawkins et al., 2017). Here, show mice dilates arterioles other regions, thus demonstrating reactivity RTN...
Chronic anticoagulation for atrial fibrillation, history of venous thromboembolism, and following heart valve replacement is often stopped or bridged surgery. Our institutional practice to continue through ambulatory phlebectomy (AP) procedures. As such, we aimed compare post-procedure bleeding major adverse events in patients on who received compared not anticoagulation. We included all required AP from January 2016 February 2023. Given the low frequency chronic during study period, as...
The proximal anastomosis is a crucial step during open repair of an abdominal aortic aneurysm. A technically sound decreases intraoperative blood loss, as well the operative time needed for suture line repair. Traditionally, continuous running used construction this anastomosis. At author's institution, technique interrupted pledgeted selectively when quality tissue suboptimal. This report describes and compares outcomes to traditional technique. Among 60 48 anastomoses, we found equivalent...
The cloaca is a transient structure that forms in the terminal hindgut giving rise to rectum dorsally and urogenital sinus ventrally. Similarly, human cultures derived from pluripotent stem cells generate colonic organoids (HCOs) which also contain co-developing urothelial tissue. In this study, our goal was identify pathways involved cloacal patterning apply cultures. RNA sequencing (RNA-seq) data comparing dorsal versus ventral e10.5 mice revealed WNT signaling elevated cloaca. Inhibition...
A longstanding hypothesis contends that sleep is promoted by sleep-active inhibitory neurons suppress arousal centers. Sleep may also be facilitated mechanisms active during wakefulness; however, evidence for wake promoting scarce. Here, we use chemogenetics, fiber photometry and circuit mapping to identify subset(s) of promote NREM sleep. We targeted the ventrolateral retropontine (VLRP) region medulla because its proximity REM found selective activation parvalbumin (VLRPPvalb) but not...
Abstract Pitt-Hopkins syndrome (PTHS) is a rare autism spectrum-like disorder characterized by intellectual disability, developmental delays, and breathing problems involving episodes of hyperventilation followed apnea. PTHS caused functional haploinsufficiency the gene encoding transcription factor 4 ( Tcf4 ). Despite severity this disease, mechanisms contributing to behavioral abnormalities are not well understood. Here, we show that truncation tr/+ ) mouse model exhibits similar patients....
Glutamatergic neurons in the retrotrapezoid nucleus (RTN) function as respiratory chemoreceptors by regulating breathing response to tissue CO 2 /H + . The RTN and greater parafacial region may also a chemosensing network composed of -sensitive excitatory inhibitory synaptic interactions. In context disease, we showed that loss neural activity mouse model Dravet syndrome disinhibited destabilized (Kuo et al., 2019). Despite this, contributions control are unknown, properties have not been...
Abstract Astrocytes in the retrotrapezoid nucleus (RTN) stimulate breathing response to CO 2 /H + , however, it is not clear how these cells detect changes . Considering Kir4.1/5.1 channels are -sensitive and important for several astrocyte-dependent processes, we consider a leading candidate sensor RTN astrocytes. To address this, show that astrocytes express Kir4.1 Kir5.1 transcripts. We also characterized respiratory function astrocyte-specific inducible knockout mice (Kir4.1 cKO);...
Abstract Loss- and gain-of-function variants in the gene encoding KCNQ2 channels are a common cause of developmental epileptic encephalopathy, condition characterized by seizures, delays, breathing problems, early mortality. To understand how dysfunction impacts behavior mouse model, we focus on control neurons expressing transcription factor Phox2b which includes respiratory ventral parafacial region. We find Phox2b-expressing express Kcnq2 absence other Kcnq isoforms, thus clarifying why...
Aneurysmal pelvic arteriovenous malformations in male patients are exceptionally rare. Upon spontaneous or traumatic rupture, these aneurysms can cause severe hemorrhage and often associated with high mortality. Given that most intact found after symptomatic presentation, other case reports have detailed an approach for elective endovascular treatment concomitant arterial venous embolization. We describe incidental discovery of a 7-cm-high flow aneurysmal malformation successful strategy...
Supervised exercise therapy (SET) provides clinical benefit for patients suffering from intermittent claudication due to peripheral artery disease. However, enrollment in programs when offered remains low. We sought identify patient-reported barriers SET as part of a prospective quality improvement program. Patients who presented clinic and were diagnosed with protocol, an effort improve completion this 12-week These at nine regional offices throughout our health system. Both enrolled...
INTRODUCTIONThe Society for Vascular Surgery(SVS) recently established the Young Surgeons Section(YSS), open to early career vascular surgeons in their first 10 years of practice and dedicated providing resources advocacy foster development, encourage diversity, equity, inclusion within society. Research was identified as a priority area focus by members this group surgeons. This study aims evaluate current contribution research presented at Annual Meeting(VAM).METHODSAbstracts plenary...