A5010257496- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Tissue Engineering and Regenerative Medicine
- Cell Image Analysis Techniques
- Bone Metabolism and Diseases
- Bone health and treatments
- Telomeres, Telomerase, and Senescence
- Bone Tissue Engineering Materials
- Fatty Acid Research and Health
- AI in cancer detection
- Cardiovascular Disease and Adiposity
- Biomedical Ethics and Regulation
- Cancer Genomics and Diagnostics
- Parathyroid Disorders and Treatments
- Medical and Biological Sciences
- Lipoproteins and Cardiovascular Health
- Mesenchymal stem cell research
- Estrogen and related hormone effects
- TGF-β signaling in diseases
- Genomics and Rare Diseases
- RNA Research and Splicing
- Bone and Dental Protein Studies
- Thyroid Disorders and Treatments
- Eicosanoids and Hypertension Pharmacology
- Translation Studies and Practices
Institute of Cytology
2022-2025
Crimea State Medical University named after S. I. Georgievsky
2024
Novosibirsk State Medical University
2024
Pathologies associated with impaired bone homeostasis, including osteoporosis, are among the leading diseases in terms of mortality. The development and implementation tissue engineering approaches based on use human mesenchymal stem cells promises to become a highly effective method for their therapy. However, fundamental cellular mechanism, which is diseases, require an additional study. Interactions between osteoblasts osteocytes undoubtedly plays important role maintaining balance...
Abstract Background Three-dimensional (3D) cell culture is widely used in various fields of biology. In comparison to conventional two-dimensional (2D) culture, 3D facilitates a more accurate replication the vivo microenvironment, which essential for obtaining relevant results. The application techniques regenerative medicine, particularly mesenchymal stem (MSC)-based research, has been extensively studied. Many these studies focus on enhanced paracrine activity MSCs cultured environments....
The ability of human pluripotent stem cells for unlimited proliferation and self-renewal promotes their application in the fields regenerative medicine. morphological assessment growing colonies cells, as a non-invasive method, allows best clones further clinical applications to be safely selected. For this purpose, we analyzed seven parameters both extracted from phase-contrast images embryonic cell line H9, control induced (hiPSC) AD3, hiPSC HPCASRi002-A (CaSR) various passages during...
G-protein-coupled receptors (GPCRs) are the largest family of cell surface receptors. They modulate key physiological functions and required in diverse developmental processes including embryogenesis, but their role pluripotency maintenance acquisition during reprogramming towards hiPSCs draws little attention. Meanwhile, it is known that more than 106 GPCRs overexpressed human pluripotent stem cells (hPSCs). Previously, to identify novel effectors reprogramming, we performed a...
Human pluripotent stem cells (hPSCs) are an important tool in the field of regenerative medicine due to their ability differentiate towards all tissues adult organism. An task study hPSCs is understand factors that influence maintenance and clonal characteristics colonies represented by morphological phenotype. Such include migrate during growth. In this work, we measured analyzed migration trajectories hPSC obtained from bright-field images three cell lines, including induced lines AD3...
Human pluripotent stem cells (hPSCs) attract tremendous attention due to their unique properties. Manual extraction of trajectories cell colonies in experimental image time series is labor intensive and subjective, thus the aim work was develop a computer semi-automated protocol for colony tracking. The developed procedure consists three major stages, namely, registration, object detection Registration using discrete Fourier transform tracking based on solution linear assignment problem...
G-protein-coupled receptors (GPCRs) have emerged as critical regulators of bone development and remodeling. In this study, we aimed to identify specific GPCR mutations in osteoporotic patients via next-generation sequencing (NGS). We performed NGS six genomic DNA samples taken from two healthy donors. Next, searched for single-nucleotide polymorphisms (SNPs) genes that are associated with osteoporosis. For three one donor, biopsies were used generate patient-specific mesenchymal stem cell...
Human pluripotent stem cells are promising for a wide range of research and therapeutic purposes. Their maintenance in culture requires the deep control their clonal status. A non-invasive method such involves day-to-day observation morphological changes, along with imaging colonies, subsequent automatic assessment colony phenotype using image analysis by machine learning methods. We developed classifier convolutional neural network applied it to discriminate between images human embryonic...
Osteoporosis is a chronic disease characterized by pathological change in bone tissue, excessive fragility and decrease strength as result of the predominance resorption processes over process formation. This manifests form low-traumatic fractures occurring falling from height one’s height, or with little physical exertion. could vertebral bodies bones peripheral skeleton, causing high level disability. Of note, postmenopausal osteoporosis develops women against background cessation...
This article is devoted to the consideration of existing level classifications equivalence, in particular, classification G. Jaeger as a method for assessing translated texts English-Russian language pair. When working with texts, methods contextual and corpus analysis were used, well synthesis identify similarities between translations. The assessed by assigning each one an appropriate level. According results assessment its help, three turned out be highest compliance original. In addition...
Osteoporosis is characterized by increased resorption and decreased bone formation; it predominantly influenced genetic factors. G-protein coupled receptors (GPCRs) play a vital role in homeostasis, mutations these genes are associated with osteoporosis. This study aimed to investigate the impact of single nucleotide polymorphism (SNP) rs1042713 ADRB2 gene, encoding beta-2-adrenergic receptor, on osteoblastogenesis. Herein, using quantitative polymerase chain reaction, western...
<title>Abstract</title> Background Three-dimensional (3D) cell culture is widely used in various fields of biology. In comparison to conventional two-dimensional (2D) culture, 3D facilitates a more accurate replication the<italic>in vivo</italic>microenvironment, which essential for obtaining relevant results. The application techniques regenerative medicine, particularly mesenchymal stem (MSC)-based research, has been extensively studied. Many these studies focus on the enhanced paracrine...