A5054772086- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Telomeres, Telomerase, and Senescence
- Peptidase Inhibition and Analysis
- RNA Interference and Gene Delivery
- Genetics, Aging, and Longevity in Model Organisms
- Virus-based gene therapy research
- Biochemical effects in animals
- Tissue Engineering and Regenerative Medicine
- Protease and Inhibitor Mechanisms
- MicroRNA in disease regulation
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Neurological Disorders and Treatments
- Heat shock proteins research
- Wound Healing and Treatments
- Historical Gender and Feminism Studies
- Adipose Tissue and Metabolism
- Yersinia bacterium, plague, ectoparasites research
- Pancreatitis Pathology and Treatment
- Biotin and Related Studies
- Human Health and Disease
- Electrospun Nanofibers in Biomedical Applications
- Erythrocyte Function and Pathophysiology
- Pancreatic function and diabetes
Institute of Cytology
2012-2024
Don State Technical University
2021
Russian Academy of Sciences
2009-2015
Asherman's syndrome (AS) is one of the gynecological disorders caused by destruction endometrium. For some cases AS available surgical methods and hormonal therapy are ineffective. Stem cell transplantation may offer a potential alternative for cure. Human endometrial mesenchymal stem cells (eMSC) organized in spheroids were transplanted rats with damaged endometrium modeled on AS. Treatment response was defined as pregnancy outcome litter size. Application eMSC significantly improved rat...
Stem cells in adult organism are responsible for cell turnover and tissue regeneration. The study of stem stress response contributes to our knowledge on the mechanisms damaged repair. Previously, we demonstrated that sublethal heat shock (HS) induced apoptosis human embryonic cells. This aimed investigate HS Human mesenchymal (MSCs) cultivated vitro were challenged with HS. It was found did not affect viability assessed by annexin V/propidium staining. However, MSCs subjected severe...
Mesenchymal stem cells are currently tested as a promising tool for the treatment of wide range human diseases. Enhanced therapeutic potential spheroids formed from these has been proved in numerous studies, however, fundamental basics this effect still being discussed. In work, we showed that endometrial mesenchymal stem/stromal (eMSCs) assembled possess higher efficacy compared to grown monolayer defects non-specific eMSC tissue origin - skin wounds. With purpose elucidate possible causes...
Embryonic stem cells (ESC) are able to self-renew and differentiate into any cell type. To escape error transmission future progeny, ESC require robust mechanisms ensure genomic stability. It was stated that stress defense of mouse human against oxidative irradiation is superior compared with differentiated cells. Here, we investigated heat shock response (hESC) their progeny. Fibroblast-like were generated by spontaneous hESC differentiation via embryoid bodies. Like normal diploid...
Quiescence is the prevailing state of many cell types under homeostatic conditions. Yet, surprisingly, little known about how quiescent cells respond to environmental challenges. The aim present study compare stress responses cycling and mesenchymal stem (MSC). Human endometrial (eMSС) were employed as adult cells. eMSC quiescence was modeled by serum starvation. Sublethal heat shock (HS) used a factor. Both heated at 45°C for 30 min then returned standard culture conditions their recovery....
The ability of human pluripotent stem cells for unlimited proliferation and self-renewal promotes their application in the fields regenerative medicine. morphological assessment growing colonies cells, as a non-invasive method, allows best clones further clinical applications to be safely selected. For this purpose, we analyzed seven parameters both extracted from phase-contrast images embryonic cell line H9, control induced (hiPSC) AD3, hiPSC HPCASRi002-A (CaSR) various passages during...
High temperature is a critical environmental and personal factor. Although heat shock well-studied biological phenomenon, hyperthermia response of stem cells poorly understood. Previously, we demonstrated that sublethal induced premature senescence in human endometrial mesenchymal (eMSC). This study aimed to investigate the fate eMSC-survived (SHS) with special emphasis on their genetic stability possible malignant transformation using methods classic molecular karyotyping, next-generation...
Temperature is an important exogenous factor capable of leading to irreversible processes in the vital activity cells. However, long-term effects heat shock (HS) on mesenchymal stromal cells (MSC) remain unstudied. We investigated karyotype and DNA repair drivers pathways human endometrium MSC (eMSC) survived progeny at passage 6 after sublethal stress (sublethal (SHS-SP)). G-banding revealed outbreak random instability caused by chromosome breakages aneuploidy. Molecular karyotyping...
In our previous study, we found that high doses of several substances with antioxidant capacities (Tempol, resveratrol, diphenyleneiodonium) can cause genotoxic stress and induce premature senescence in the human mesenchymal stem cells (MSCs). Here, using whole-transcriptome analysis, revealed signs endoplasmic reticulum unfolded protein response (UPR) MSCs stressed Tempol resveratrol. addition, upregulation genes, coding UPR downstream target APC/C, E3 ubiquitin ligase regulate stability...
The mechanisms underlying the therapeutic potential of MSCs are focus intense research. We studied human isolated from desquamated endometrium (eMSCs), which, as previously shown, have high regenerative in various disease models. aim was to evaluate role secreted VEGF stimulating angiogenesis and maintaining eMSC viability migration, which is important for improving properties MSCs. compared three cultures differing level secretion: 3D spheroids, monolayer eMSCs, eMSCs with knockdown....
Endometrial mesenchymal stem/stromal cells (eMSCs) hold great promise in bioengineering and regenerative medicine due to their high expansion potential, unique immunosuppressive properties multilineage differentiation capacity. Usually, eMSCs are maintained applied as a monolayer culture. Recently, using animal models with endometrial skin defects, we showed that formation of multicellular aggregates known spheroids from enhances tissue repair capabilities. In this work, refined method...
This study describes the changes in ion homeostasis of human endometrial mesenchymal stem/stromal cells (eMSCs) during formation three-dimensional (3D) cell structures (spheroids) and investigates conditions for apoptosis induction 3D eMSCs. Detached from monolayer culture, (2D) eMSCs accumulate Na+ have dissipated transmembrane gradients, while compact spheroids, restore lower content high K/Na ratio characteristic functionally active cells. Organized as are non-proliferating with an Na/K...
The synthetic polymer, polyallylamine hydrochloride (PAA), is found in a variety of applications biotechnology and medicine. It used gene siRNA transfer, to form microcapsules for targeted drug delivery damaged tumor cells. Conventional chemotherapy often does not kill all cancer cells leads multidrug resistance (MDR). Until recently, studies the effects PAA on have mainly focused their morphological genetic characteristics immediately or several hours after exposure polymer. properties cell...