A5072645530- Adipose Tissue and Metabolism
- Muscle metabolism and nutrition
- Muscle Physiology and Disorders
- Exercise and Physiological Responses
- Mitochondrial Function and Pathology
- Turbomachinery Performance and Optimization
- Biochemical effects in animals
- Refrigeration and Air Conditioning Technologies
- Protease and Inhibitor Mechanisms
- Glycosylation and Glycoproteins Research
- Microtubule and mitosis dynamics
- Autophagy in Disease and Therapy
- Signaling Pathways in Disease
- Monoclonal and Polyclonal Antibodies Research
- Nutrition and Health in Aging
- Peptidase Inhibition and Analysis
- Muscle activation and electromyography studies
- Medical Image Segmentation Techniques
- Cell death mechanisms and regulation
- Heat Transfer Mechanisms
- Genetics and Physical Performance
- Cardiovascular and exercise physiology
- Diet and metabolism studies
- Cardiomyopathy and Myosin Studies
- Transcranial Magnetic Stimulation Studies
Nippon Sport Science University
2017-2024
National Hospital Organization Takasaki Medical Center
2024
Tokyo University of Agriculture and Technology
2024
Shibaura Institute of Technology
2022-2023
The University of Tokyo
2013-2022
Saiseikai Ibaraki Hospital
2022
Kansai Medical University
2022
Kyoto Tachibana University
2021
Okayama University of Science
2020
York University
2017-2019
Oxidative stress and mitochondrial dysfunction are associated with the aging process. However, role of nuclear factor erythroid 2 -related (Nrf2) in skeletal muscle during remains to be clarified. In current study, we assessed whether lack Nrf2, which is known as a master regulator redox homeostasis, promotes age-related atrophy muscle. Here, demonstrated that 4-hydroxynonenal protein carbonyls, markers oxidative stress, were robustly elevated aged Nrf2 knockout (KO) mice because decreased...
Bcl‐2 protein play important roles in the regulation of apoptosis. We previously reported that phosphorylation was augmented by treatment with phosphatase 2A (PP2A) inhibitor; however, kinase responsible for had not yet been identified. In this study, we identified extracellular‐signal‐regulated (ERK) as Bcl‐2. also found transmembrane region (TM) deleted form (Bcl‐2ΔTM), which unable to localize on mitochondria constitutively phosphorylated, whereas wild‐type localized mitochondria, present...
To examine the potential role of lactate as a signalling molecule in skeletal muscle, we performed global gene expression analysis mouse gastrocnemius 3 h after administration using Affymetrix GeneChip system (Affymetrix, Santa Clara, Calif., USA). Among top 15 genes with largest fold change, increased Ppargc1a, Pdk4, and Ucp3 was confirmed real-time quantitative polymerase chain reaction. Our findings suggest that serves signal for upregulating related to mitochondrial function.
A recent study demonstrated that heat stress induces mitochondrial biogenesis in C2C12 myotubes, thereby implying may be an effective treatment to enhance endurance training-induced adaptations skeletal muscle. However, whether actually muscle vivo is unclear. In the present study, we report novel findings 1) whole body produced by exposure of ICR mice a hot environment (40°C, 30 min/day, 5 days/wk, 3 wk) induced such as increased enzyme activity (citrate synthase and 3-hydroxyacyl CoA...
Traumatic nerve injury or disease leads to denervation and severe muscle atrophy. Recent evidence shows that mitochondrial loss could be a key mediator of skeletal Here, we show daily heat stress treatment rescues denervation-induced mitochondria concomitant We also found denervation-activated autophagy-dependent clearance (mitophagy) was suppressed by treatment. The molecular basis this observation is explained our results showing attenuates the increase proteins regulate tagging step for...
SUMMARY Abscisic acid (ABA) is a phytohormone that promotes leaf senescence in response to environmental stress. We previously identified methyl CpG‐binding domain 10 (MBD10) as phosphoprotein becomes differentially phosphorylated after ABA treatment Arabidopsis. ABA‐induced was delayed mbd10 knockout plants but accelerated MBD10 ‐overexpressing plants, suggesting positively regulates senescence. phosphorylation of occurs planta on Thr‐89, and our results demonstrated Thr‐89 essential for...
Duchenne muscular dystrophy (DMD) is an X-linked genetic disorder characterized by progressive weakness because of the loss dystrophin. Extracellular Ca2+ flows into cytoplasm through membrane tears in dystrophin-deficient myofibers, which leads to muscle contracture and necrosis. Sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) takes up cytosolic sarcoplasmic reticulum, but its activity decreased dystrophic muscle. Here, we show that allosteric SERCA activator, CDN1163, ameliorates...
Dendritic cells (DCs) loaded with tumour antigens have been successfully used to induce protective immunity in murine models and human trials. However, it is still unclear which DC administration route elicits a superior therapeutic effect. Herein, we investigated the vaccine efficiency of DC2.4 cells, dendritic cell line, pulsed ovalbumin (OVA) E.G7-OVA model after immunization via various routes. After single vaccination using 1 × 106 OVA-pulsed was completely rejected intradermally (i.d.;...
It is well known that resistance exercise increases muscle protein synthesis and strength. However, little about the effect of on mitochondrial dynamics, which coupled with function. In skeletal muscle, mitochondria exist as dynamic networks are continuously remodeling through fusion fission. The purpose this study was to investigate acute chronic exercise, induces hypertrophy, expression proteins related dynamics in rat muscle. Resistance consisted maximum isometric contraction, induced by...
Abstract High‐intensity exercise stimulates glycolysis, subsequently leading to elevated lactate production within skeletal muscle. While produced the muscle is predominantly released into circulation via monocarboxylate transporter 4 (MCT4), recent research underscores lactate's function as an intercellular and intertissue signalling molecule. However, its specific intracellular roles cells remains less defined. In this study, our objective was elucidate effects of increased intramuscular...
Abstract RECK, a glycosylphosphatidylinositol (GPI)–anchored glycoprotein, negatively regulates matrix metalloproteinases (MMP), such as MMP-9, and inhibits tumor invasion metastasis. The predicted amino acid sequence of human RECK includes five putative N-glycosylation sites; however, the precise biochemical role glycosylated remains unknown. In this study, we examined link between glycosylation function in cell lines. protein was at Asn86, Asn200, Asn297, Asn352 residues but not Asn39...
Recent studies suggested that lactate accumulation can be a signal for mitochondrial biogenesis in skeletal muscle. We investigated whether reductions concentrations response to dichloroacetate (DCA), an activator of pyruvate dehydrogenase, attenuate adaptations after exercise training mice. first confirmed DCA administration (200 mg/kg BW by i.p. injection) 10 min before decreased muscle and blood high-intensity interval (10 bouts 1 treadmill running at 40 m/min with rest). At the same...
This paper describes the flow mechanisms of rotating stall inception in a multistage axial compressor an actual gas turbine. Large-scale numerical simulations unsteady have been conducted. The investigated is test rig that was used development Kawasaki L30A industrial While consists total 14 stages, only front stages were analyzed present study. data show fifth or sixth machine are most likely ones leading to stall. To model precise physics inception, modeled using very dense computational...
Abstract Oxidative stress‐induced mitochondrial dysfunction is associated with age‐related and disuse‐induced skeletal muscle atrophy. However, the role of nuclear factor erythroid 2‐related 2 (Nrf2) during fiber atrophy remains to be elucidated. In this study, we examined whether deficiency Nrf2, a master regulator antioxidant transcription, promotes denervation‐induced fragmentation We found that expression Nrf2 its target genes was upregulated at weeks after denervation in wild‐type ( WT...
Mechanical unloading impairs cytosolic calcium (Ca2+) homeostasis in skeletal muscles. In this study, we investigated whether sarco(endo)plasmic reticulum Ca2+-ATPase (SERCA) itself or one of the regulators Ca2+ SERCA pump, sarcolipin (SLN), is altered to deregulate cast immobilized, atrophied Hindlimb muscles 8-wk-old male C57BL/6J mice were subjected bilateral immobilization for 2 wk. Two-week-cast induced both body weight and muscle loss. Highly phosphorylated Ca2+/calmodulin-dependent...
High-intensity muscle contractions (HiMCs) are known to increase c-Myc expression that is stimulate ribosome biogenesis and protein synthesis in most cells. However, although mRNA transcription translation have been shown be upregulated following resistance exercise concomitantly with increased biogenesis, this connection has not tested directly. We investigated the effect of adeno-associated virus (AAV)-mediated overexpression, or without fasting percutaneous electrical stimulation-induced...
The contraction of myotubes using electrical pulse stimulation is a research tool used to mimic muscle contractile activity and exercise in rodents humans. Most protocols employed previous work low-frequency twitch contractions. However, high-frequency tetanus contractions that are more physiologically relevant vivo poorly characterized. In this report, the similarities differences acute responses chronic adaptations with different modes twitches (2 Hz, continuous, 3 h) (66 on: 5 s/off: s,...
Abstract Belt electrode-skeletal muscle electrical stimulation (B-SES) involves the use of belt-shaped electrodes to contract multiple groups simultaneously. Twitch contractions have been demonstrated protect against denervation-induced atrophy in rats, possibly through mitochondrial biosynthesis. This study examined whether inducing tetanus with B-SES suppresses and identified underlying molecular mechanisms. We evaluated effects acute (60 Hz, 5 min) chronic min, every alternate day for one...
Recently, the demand for thermoplastic elastomers (TPEs) with high tensile strength and strong toughness has grown. In this study, we synthesized ionically-functionalized polystyrene-b-polyisoprene-b-polystyrene (i-SIS(n)) containing 7.1 mol% of monosodium succinate...