A5002601563- RNA and protein synthesis mechanisms
- Biotin and Related Studies
- Alzheimer's disease research and treatments
- Ultrasound and Hyperthermia Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Amino Acid Enzymes and Metabolism
- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- Mitochondrial Function and Pathology
- Photoacoustic and Ultrasonic Imaging
- Advanced Proteomics Techniques and Applications
- Axon Guidance and Neuronal Signaling
- Sphingolipid Metabolism and Signaling
- Anesthesia and Neurotoxicity Research
- Protein Structure and Dynamics
- RNA Research and Splicing
- Nerve injury and regeneration
- RNA modifications and cancer
- S100 Proteins and Annexins
- RNA regulation and disease
- Medicinal Plants and Neuroprotection
- Receptor Mechanisms and Signaling
- Memory and Neural Mechanisms
- Cell death mechanisms and regulation
New York University
2023
Park Centre for Mental Health
2017-2021
The University of Queensland
2016-2021
Alzheimer's disease is characterized by the deposition of amyloid-β as extracellular plaques and hyperphosphorylated tau intracellular neurofibrillary tangles. Tau pathology characterizes not only disease, but also many other tauopathies, presenting an attractive therapeutic target. Passive immunotherapy has been previously explored; however, because a small fraction peripherally delivered antibodies crosses blood–brain barrier, enters brain engages with that forms aggregates, more efficient...
Tau is a scaffolding protein that serves multiple cellular functions are perturbed in neurodegenerative diseases, including Alzheimer's disease (AD) and frontotemporal dementia (FTD). We have recently shown amyloid-β, the second hallmark of AD, induces de novo synthesis tau. Importantly, this activation was found to be tau-dependent, raising question whether FTD-tau by itself affects synthesis. therefore applied non-canonical amino acid labelling visualise identify newly synthesised proteins...
The synthesis of new proteins is a fundamental aspect cellular life and required for many neurological processes, including the formation, updating extinction long-term memories. Protein impaired in neurodegenerative diseases tauopathies, which pathology caused by aberrant changes to microtubule-associated protein tau. We recently showed that both global de novo select ribosomal (RPs) are decreased mouse models frontotemporal dementia (FTD) express mutant forms However, comprehensive...
Abstract Advanced physiological aging is associated with impaired cognitive performance and the inability to induce long-term potentiation (LTP), an electrophysiological correlate of memory. Here, we demonstrate in physiologically aged, senescent mouse brain that scanning ultrasound combined microbubbles (SUS +MB ), by transiently opening blood–brain barrier, fully restores LTP induction dentate gyrus hippocampus. Intriguingly, SUS treatment without only i.e., uptake blood-borne factors,...
The formation of spatial long-term memory (LTM) requires the de novo synthesis distinct sets proteins; however, a non-biased examination proteome in this process is lacking. Here, we generated novel mouse strain, which enables cell-type-specific labelling newly synthesised proteins with non-canonical amino acids (NCAAs) by genetically restricting expression mutant tRNA synthetase, NLL-MetRS, to hippocampal neurons. By combining technique an accelerated version active place avoidance task and...
Abstract Phosphatase and tensin homolog (PTEN) regulates synaptic density in development; however, whether PTEN also synapse loss a neurodegenerative disorder such as frontotemporal lobar degeneration with Tau deposition (FTLD-Tau) has not been explored. Here, we found that pathological promotes early activation of PTEN, which precedes apoptotic caspase-3 cleavage the rTg4510 mouse model FTLD-Tau. We further demonstrate increased neuronal exposure signal phosphatidylserine tags structures...
Age is a critical factor in the prevalence of tauopathies, including Alzheimer's disease. To observe how an aging phenotype interacts with and affects pathological intracellular accumulation hyperphosphorylated tau, tauopathy mouse model pR5 (expressing P301L mutant human tau) was back-crossed more than ten times onto senescence-accelerated SAMP8 background to establish new strain, SApT. Unlike mice, mice are characterized by robust tau pathology particularly amygdala hippocampus. Analysis...
Abstract The formation of new associative long-term memory (LTM) following Pavlovian conditioning is dependent upon multiple, temporally distinct windows mRNA translation. Current methods lack the temporal specificity to robustly characterize dynamics protein synthesis throughout rodent brain conditioning. Here we resolve these technological limitations and demonstrate that in awake mice, retro-orbital (RO) injection azidohomoalanine (AHA) enables labelling subsequent visualization de novo...
Abstract Background Protein synthesis is a vital biological process, important for many neuronal and cognitive processes such as synaptic plasticity the formation, updating, extinction of long‐term memories. Recent studies have identified dysregulated translation molecular hallmark neurodegenerative diseases, including tauopathies Alzheimer’s disease (AD), frontotemporal dementia (FTD), corticobasal degeneration (CBD) (Evans et al., EMBO J, 2019; Evans Acta Neuropathoc Comms, 2021; Elder...
Abstract Background Tau is a scaffolding protein which serves multiple cellular functions that are perturbed in neurodegenerative diseases, including Alzheimer’s disease (AD) and frontotemporal dementia (FTD). Recently we demonstrated amyloid‐b, the second hallmark of AD, induces de novo synthesis tau, with this activation being found to be tau‐dependent. This raised question whether FTD‐tau by itself affects synthesis. Method In order examine synthesis, optimised non‐canonical amino acid...