A5022632543- Immune Cell Function and Interaction
- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- Glycosylation and Glycoproteins Research
- Immune cells in cancer
- T-cell and B-cell Immunology
- Liver physiology and pathology
- Platelet Disorders and Treatments
- Galectins and Cancer Biology
- Pancreatic function and diabetes
- Cytokine Signaling Pathways and Interactions
- Liver Disease Diagnosis and Treatment
- CAR-T cell therapy research
- Diabetes and associated disorders
- Immune Response and Inflammation
- Epigenetics and DNA Methylation
- Diet and metabolism studies
- Hepatitis B Virus Studies
- Cancer, Hypoxia, and Metabolism
- RNA Interference and Gene Delivery
- Complement system in diseases
- Phagocytosis and Immune Regulation
- Connexins and lens biology
- PI3K/AKT/mTOR signaling in cancer
- Fibroblast Growth Factor Research
National Yang Ming Chiao Tung University
2013-2024
National Yang Ming University Hospital
2017
University of British Columbia
2010-2011
National Taiwan University
2003-2010
Institute of Biological Chemistry, Academia Sinica
2004-2006
Mackay Memorial Hospital
2000-2004
New York Blood Center
2003
Drexel University
2003
Chang Gung Memorial Hospital
2003
Hepatocellular carcinoma (HCC) is the most common primary malignant tumor worldwide; however, traditional therapeutic approaches and survival rates are still limited. To improve current therapies, it necessary to investigate molecular mechanisms underlying liver cancer identify potential targets. The aims of this study were verify ketogenesis rate-limiting enzyme 3-Hydroxymethylglutaryl-CoA synthase 2 (HMGCS2) in HCC. Immunohistochemical staining human disease tissue arrays showed that...
Phthalates are often added to plastic products increase their flexibility. Di-(2-ethylhexyl) phthalate (DEHP) is one of the most common plasticizers. Previously, a major incident involving phthalate-contaminated foodstuffs occurred, where phthalates were deliberately as substitute for emulsifiers, resulting in threat public health. DEHP exposure can cause liver damage and further lead cancer; however, effects long-term low-dose on hepatic stellate cells (HSCs) fibrosis still unclear. In this...
Members of the TNF and receptor (TNFR) superfamily play important roles in maintenance homeostasis immune system. Furthermore, several members TNFR family participate T-cell activation sustaining responses. We have shown that TNFR2 regulates by lowering threshold providing costimulatory signaling. activated TNFR2(-/-) CD8(+) T cells are highly resistant to activation-induced cell death (AICD). Here, we showed using anti-TNFR2 antibodies block on WT rendered them AICD. This resistance AICD...
In chronic liver diseases, regardless of their etiology, the development fibrosis is first step toward progression to cirrhosis, portal hypertension, and hepatocellular carcinoma. Hepatic stellate cells (HSCs) are main profibrogenic that promote pathogenesis fibrosis, so it important identify molecules regulate HSCs activation fibrosis. Niemann-Pick type C2 (NPC2) protein plays an role in regulation intracellular cholesterol homeostasis by directly binding with free cholesterol. However,...
The Kell blood group system is polymorphic, and 23 antigens have been defined to date. are located on a single red cell transmembrane glycoprotein, encoded by the 19 exons of KEL gene. different phenotypes result from point mutations leading amino acid changes in glycoprotein. An unusual phenotype, which as complete lack all antigens, has identified designated Kell-null or Ko phenotype. coding region gene individual showed normal KEL2/KEL4/KEL7 sequence; nevertheless, G C mutation at splice...
It has been noted that the expression of Sda, including its antigenic structure, β-1,4-N-acetylgalactosyltransferase II (β4GalNAcT-II) activity responsible for formation, and Sda β4GalNAcT-II mRNA transcript, is drastically reduced in oncogenetic processes gastrointestinal tissues. Markedly metastatic potential demonstrated colon gastric cancer cells transfected with gene. In this study, a putative CpG island encompassing promoter exon 1 regions human gene was identified, investigation DNA...
Liver fibrosis is the first step toward progression to cirrhosis, portal hypertension, and hepatocellular carcinoma. A high-cholesterol diet associated with liver via accumulation of free cholesterol in hepatic stellate cells (HSCs). Niemann-Pick type C2 (NPC2) plays an important role regulation intracellular homeostasis direct binding cholesterol. Previously, we reported that NPC2 was downregulated cirrhosis tissues. Loss enhanced HSCs made them more susceptible transforming growth factor...
BACKGROUND The P 1 /P 2 phenotypic polymorphism is one of the earliest blood groups discovered in humans. These have been connected to different levels expression A4GALT gene and red cells; however, detailed molecular genetic mechanism that leads these two phenotypes has not established. STUDY DESIGN AND METHODS After our previous identification an association between single‐nucleotide polymorphisms (SNPs) rs2143918 rs5751348 phenotype, we conduct a survey transcription factors might connect...
Altered sialic acid processing that leads to upregulation of cell surface sialylation is recognized as a key change in malignant tissue glycosylation. This cancer-associated hypersialylation directly impacts the signaling interactions between tumor cells and their surrounding microenvironment, especially mediated by immune acid-binding immunoglobulin-like lectins (Siglecs) relay inhibitory signals for cytotoxicity. First, we obtained Siglec-7neg NK-92MI line, NK-92MI-S7N, separating group...
Glycine N-methyltransferase (GNMT) is abundantly expressed in the normal liver but down-regulated cancer tissues. GNMT knockout (Gnmt−/−) mice can spontaneously develop chronic hepatitis, fatty liver, and cancer. We previously demonstrated that hepatic decreased high-fat-diet-induced type 2 diabetes mellitus, its contribution to metabolic syndrome unclear. Here we show modulates key aspects of mice. Eleven-week-old Gnmt−/− wild-type (WT) with a C57BL/6 genetic background were used this...
BACKGROUND: In addition to the common ABO phenotypes, numerous phenotypes with a weak expression of A or B antigens on RBCs have been found. This study describes molecular genetic analysis el and 3 phenotypes. STUDY DESIGN AND METHODS: The seven‐exon regions genes individuals were amplified by PCR cloned, sequences exons their adjacent splice sites analyzed. Samples from 30 randomly selected 1 also assessed. RESULTS: gene wild‐type coding sequence was demonstrated in propositus, but all six...
BACKGROUND: It has been demonstrated that the 43‐bp minisatellite sequence in 5′ region of ABO gene plays an important role its transcriptional regulation. was determined previous investigations structure enhancer specific to A , B and O alleles. STUDY DESIGN AND METHODS: Real‐time polymerase chain reaction (PCR) detection a PCR‐restriction fragment length polymorphism (RFLP) strategy were used compare quantities transcripts AB ‐genotype cells, including peripheral blood cells cancer cell...
Hepatocellular carcinoma (HCC) is one of the most prevalent malignancies and deadliest cancers in world.
The increased proliferation and activation of hepatic stellate cells (HSCs) are associated with liver fibrosis development. To date, there no FDA-approved drugs for the treatment cirrhosis. Augmentation HSCs apoptosis is one resolutions fibrosis. In this study, we extracted α-mangostin (1,3,6-trihydroxy-7-methoxy-2,8-bis(3-methyl-2-butenyl)-9H-xanthen-9-one) from fruit waste components mangosteen pericarp. isolated structure was determined characterized nuclear magnetic resonance (NMR)...
The Secretor gene (Se or FUT2), which produces alpha(1,2)-fucosyltransferase, exhibits extensive polymorphism. Six Se genes, including the weak (Se(w) Se385) and three nonsecretor alleles (se571, se685, se849) have been detected in various populations of Taiwan. distribution Lewis phenotypes among Taiwanese population groups has shown to vary considerably.A PCR-RFLP analysis system, was based on nucleotide polymorphism variation different can reveal genotype an individual easily accurately,...
Perturbation of gut microbiota has been linked to chronic kidney disease (CKD), which was correlated with a sophisticated milieu metabolic and immune dysregulation.