A5015152606- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- CAR-T cell therapy research
- Immune cells in cancer
- RNA modifications and cancer
- Hepatocellular Carcinoma Treatment and Prognosis
- Physiological and biochemical adaptations
- Cancer, Lipids, and Metabolism
- Peptidase Inhibition and Analysis
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- Histone Deacetylase Inhibitors Research
- Cancer, Hypoxia, and Metabolism
- Cervical Cancer and HPV Research
- Radiomics and Machine Learning in Medical Imaging
- Nanoparticle-Based Drug Delivery
- Cancer Genomics and Diagnostics
- Inflammatory Biomarkers in Disease Prognosis
- Cancer-related Molecular Pathways
- Chronic Lymphocytic Leukemia Research
- Insect-Plant Interactions and Control
Wuxi No.2 People's Hospital
2022-2025
Nanjing Medical University
2022-2025
Sichuan University
2015-2024
Jiangnan University
2023-2024
Twelfth Guangzhou City People's Hospital
2024
Shenzhen Second People's Hospital
2014-2024
State Key Laboratory of Biotherapy
2015-2023
Nanjing University of Chinese Medicine
2023
Jiangsu Province Hospital
2023
University Medical Center
2023
Nanocarriers with positive surface charges are known for their toxicity which has limited clinical applications. The mechanism underlying toxicity, such as the induction of inflammatory response, remains largely unknown. In present study we found that injection cationic nanocarriers, including liposomes, PEI, and chitosan, led to rapid appearance necrotic cells. Cell necrosis induced by nanocarriers is dependent on charges, but does not require RIP1 Mlkl. Instead, intracellular Na+ overload...
Here, we sought to explore the underlying role of interleukin (IL)-8 in neutrophil extracellular traps (NETs) formation during atherosclerosis (AS). The concentration pro-inflammatory cytokines IL-8, IL-6 and IL-1β was determined by enzyme-linked immunosorbent assay (ELISA). NETs evaluated immunofluorescence myeloperoxidase (MPO)-DNA complex ELISA. mRNA levels IL-8 Toll-like receptor 9 (TLR9) were measured quantitative real-time PCR (qRT-PCR). phosphorylation NF-κB p65 detected western...
Abstract The existence of breast cancer stem cells (BCSCs) is a major reason underlying metastasis and recurrence after chemotherapy radiotherapy. Targeting BCSCs may ameliorate relapse therapy resistance. Here we report that expression the pseudokinase Tribble 3 (TRIB3) positively associates with stemness progression. Elevated TRIB3 supports by interacting AKT to interfere FOXO1-AKT interaction suppress FOXO1 phosphorylation, ubiquitination, degradation E3 ligases SKP2 NEDD4L. accumulated...
Fibroblast activation protein (FAP) is highly expressed in the tumor‐associated fibroblasts (TAFs) of most human epithelial cancers. FAP plays a critical role tumorigenesis and cancer progression, which makes it promising target for novel anticancer therapy. However, mere abrogation enzymatic activity by small molecules not very effective inhibiting tumor growth. In this study, we have evaluated immune‐based approach to specifically deplete FAP‐expressing TAFs mouse 4T1 metastatic breast...
Abstract λ-Carrageenan is a seaweed polysaccharide which has been generally used as proinflammatory agent in the basic research, however, how immunomodulating activity of λ-carrageenan affects tumor microenvironment remains unknown. In this study, we found that intratumoral injection could inhibit growth B16-F10 and 4T1 bearing mice enhance immune response by increasing number tumor-infiltrating M1 macrophages, DCs more activated CD4 + CD8 T lymphocytes spleen. addition, secretion IL17A...
Chimeric antigen receptor-modified T cells (CAR-T cells) have emerged as a promising cancer immunotherapy for solid tumors. Epithelial cell adhesion molecule (EpCAM) is overexpressed in variety of tumors and recognized biomarker circulating tumor stem cells, representing an attractive target adoptive T-cell immunotherapy. This study generated third-generation CAR-T with redirected specificity to EpCAM (EpCAM CAR-T) by lentiviral vector. The demonstrated that can elicit lytic cytotoxicity...
Abstract Background Understanding how the tumor microenvironment is shaped by various factors important for development of new therapeutic strategies. Tumor cells often undergo spontaneous apoptotic cell death in microenvironment, these are histologically co‐localized with immunosuppressive macrophages. However, mechanism which apoptosis modulates macrophage polarization not fully understood. In this study, we aimed to explore promoting effects and signal pathways involved. Methods Apoptotic...
The efficacy of CAR-T cells for solid tumors is unsatisfactory. EpCAM a biomarker epithelial tumors, but the clinical feasibility therapy targeting lacking. Here, we report pre- and investigations EpCAM-CAR-T tumors. We demonstrated that costimulated by Dectin-1 exhibited robust antitumor activity without adverse effects in xenograft mouse models EpCAM-humanized mice. Notably, trials (NCT02915445), 6 (50%) 12 enrolled patients experienced self-remitted grade 1/2 toxicities, 1 patient (8.3%)...
Background: Cisplatin is a widely used anti-tumor agent but its use frequently limited by nephrotoxicity.Transient receptor potential melastatin 2 (TRPM2) non-selective cation channel which generally viewed as sensor of oxidative stress, and increasing evidence supports link with autophagy, critical process for organelle homeostasis.Methods: Cisplatin-induced cell injury mitochondrial damage were both assessed in WT Trpm2-knockout mice primary cells.RNA sequencing, immunofluorescence...
Attempts to develop cell-based cancer vaccines have shown limited efficacy, partly because transplanted dendritic cells (DCs) do not survive long enough reach the lymph nodes. The development of biomaterials capable modulating DCs in situ enhance antigen uptake and presentation has emerged as a novel method toward developing more efficient vaccines. Here, we propose two-step hybrid strategy produce robust vaccine situ. First, significant number are recruited an injectable thermosensitive...
The unique spectral properties of semiconductor quantum dots (QDs) enable long-term live-cell imaging and ultrasensitive detection viral particles, which in turn can potentially provide a practical means for detailed analysis the underlying molecular mechanisms virus entry. In this study, we report general method labeling adeno-associated serotype 2 (AAV2) with QDs enhanced visualization intracellular behavior viruses living target cells. It was found that mild conditions required QD...
Background Non-small cell lung cancer (NSCLC) is a leading cause of death worldwide. Early diagnosis essential for improvements prognosis and survival the patients. Currently, there no effective biomarker available in clinical settings early detection cancer. Altered expressions many types including NSCLC stable existence plasma make microRNAs (miRNAs) group potentially useful biomarkers assessments patients with NSCLC. Objectives To evaluate potential values miRNAs as blood-based Methods...
Zwint, a centromere-complex component required for the mitotic spindle checkpoint, has been reported to be overexpressed in different human cancers, but it not studied hepatocellular carcinoma (HCC).The role of Zwint cell proliferation capacities was evaluated by using counting kit-8 (CCK8), flow cytometry, clone formation and tumor assay nude mice. Western blot analysis qPCR were performed assess interacting with cell-cycle-related proteins.We report that ZWINT mRNA protein expression...
Background Locally advanced rectal cancer (LARC) patient stratification by clinicoradiologic factors may yield variable results. Therefore, more efficient prognostic biomarkers are needed for improved risk of LARC patients, personalized treatment, and prognostication. Purpose/Hypothesis To compare the ability a radiomic signature to predict disease‐free survival (DFS) with that model in individual patients LARC. Study Type Retrospective study. Population In all, 108 consecutive (allocated...
The neutrophil-lymphocyte ratio (NLR) and the platelet-lymphocyte (PLR) have been proved to affect prognosis of various types cancers. However, prognostic role NLR PLR in patients with small-cell lung cancer (SCLC) remains controversial. objective this study is assess values NLR, other potential indexes SCLC patients.The optimal cutoff levels were 2.65 for 125 210 LDH by ROC curves analysis. Patients ≥ groups significantly correlated worse PFS OS. < group presented longer time than group....
Abstract The objective of this study was to reveal a novel mechanism underlying the progression atherosclerosis (AS) associated with endothelial cells (ECs) and neutrophils. Transmission electron microscopy (TEM) nanoparticle tracking analysis (NTA) were used observe morphology particle size isolated exosomes. Western blotting applied examine exosomal markers, while expression metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) measured by quantitative real-time polymerase chain...