The conversion of testosterone into oestradiol by ovine Leydig cells culturedin vitrowas studied using the non-steroidal aromatase inhibitor CGS 16949A. Additionally, activity was detected immunohistochemical staining cultured or cryosections. were obtained from testes Polish Mountain rams 5–6 months old (immature) 12–15 (mature). alone (controls) incubated for 6 h in presence testosterone. Aromatase then added to cultures which a further 18 h. After 24-h incubation period, and secretion...

Angiogenesis is a multi-stage process of new vessel development which involves migration, proliferation and differentiation endothelial cells. Pathological angiogenesis plays crucial role in the pathomechanism various ischemic, malignant inflammatory disorders. Among eye diseases, macular degeneration (AMD) proliferative diabetic retinopathy are major public health issue as most common causes blindness. Since these conditions, there has been an increased interest evaluating anti-angiogenic...

Adipocytes derived from 3T3-L1 cells are a gold standard for analyses of adipogenesis processes and the metabolism fat cells. A widely used histological immunohistochemical staining mass spectrometry lipidomics mainly aimed examining lipid droplets (LDs). Visualizing other cellular compartments contributing to machinery requires additional cell culturing multiple labeling. Here, we present localization intracellular structure 3T3-L1-derived adipocytes utilizing vibrational spectromicroscopy,...

The hexachlorophene (HCP) is a highly lipophilic chlorinated bisphenol present in hygienic and dermatological products. HCP accumulates preferentially adipose tissue that privileged source of mesenchymal stem cells (MSCs). evaluation the potential effects on MSCs important for their medical application. Here we examined murine tissue-derived (ADSCs) human umbilical cord-derived (UCSCs) cell culture. We found 10−4 10−5 M inhibits proliferation, osteogenesis increases apoptosis ADSCs UCSCs....

Protein undernutrition affects inter alia blood parameters and immune system. These negative effects may be improved by the addition of vitamin B6 to diet. Here, we evaluated effect supplementation physical exercise in rats fed a protein-deficient Rats were divided into six groups: exercised or unexercised – control, protein deficient, deficient with supplementation. Sixty days malnutrition caused significant changes rat body weight, haematological (mainly red cells parameters),...

The genus Rhodiola (Crassulaceae) consists of many species, growing mainly in Asia and traditionally used as adaptogens anti-inflammatory drugs. In order to elaborate herbal immunostimulator which could be safely given pregnant women, we performed a study on immunotropic effects feeding lactating mice kirilowii extracts. This paper presents the results first part our - spleen content selected polyphenols, cellularity, splenocytes phenotype their response mitogens. Experiments were adult...

Escherichia coli is a common cause of infections in companion animals. In recent years the increasing prevalence resistance to β-lactams, including extended-spectrum cephalosporins, antimicrobials frequently used small animal veterinary practice, was observed canine isolates E. coli. The aim this study detect and characterize β-lactamases (ESBLs) produced by isolated from diseased dogs Poland. Four out 119 studied (3.4%) were ESBL-positive. They harbored blaSHV-12, blaCTX-M-15, blaTEM-116...

Liposomes are used for encapsulation of the active compounds in different therapies, with increasing frequency. The important areas clinical applications liposomes cancer targeted treatment, antibiotic delivery or regenerative medicine. can transfer both hydrophilic and hydrophobic have lipid bilayer which imitates cell membrane. additionally may extend half-live period drugs protect them against elimination ways, such as phagocytosis, enzymatic cleavage exclusion by detoxification. size...

Mesenchymal stem cells (MSCs) are used as therapeutic agents for the treatment of a wide spectrum diseases, well regeneration and healing burns wounds. MSCs have an immunomodulatory effect influence phenotype functions immune cells, including macrophages, which in turn prime license MSCs. We discuss new findings on feedback loop between macrophages its consequences outcome MSC therapies.

Research on the implementation of patient decision aids to facilitate shared making in clinical settings has steadily increased across Western countries. A study which implements and measures their impact yet be conducted Eastern part Europe.To use Option GridTM a sample Grupa LUX MED clinics Warsaw, Poland, measure making.We pre-post interventional study. Following three-month period usual care, clinicians from three received one-hour training session how were provided with copies for four...

Vitamin B6 is necessary for many enzymatic pathways (glucose and lipid metabolism, DNA/RNA synthesis, or modulation of gene expression) affects immune cell function blood-forming processes. We hypothesised that supplementing a protein-deficient diet with vitamin may reduce the negative impact protein malnutrition. Here, we evaluated effect moderate, long-term exercise (ninety days) on selected blood parameters in rats fed normal diet, supplemented B6. Selected haematological, immunological,...

Abstract Circulating endothelial cell progenitors originating from the bone marrow are considered to be a powerful tool in repair of endothelium damage. Due their unique properties, now broadly investigated assess clinical significance diseases e.g., associated with brain dysfunction. However, distinction terms expression specific markers remains ambiguous. Additionally, progenitor cells may change repertoire depending on microenvironment tissue which they currently located. Here, we applied...

In these studies, a clone of cells derived from the porcine renal epithelial line LLC-PK1 grown on porous filters was used to evaluate basolateral uptake organic cation tetraethylammonium (TEA). (3H) TEA (1 microM) entered in saturable and time-dependent manner achieving steady-state value at 2 2.5 h. Uptake reduced by hypothermia metabolic inhibitors sodium azide iodoacetate. Several other cations 1 mM concentrations inhibited majority uptake. lower concentrations, inhibitory potency was:...

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It is very difficult to cure pregnant females suffering from infections, because of the risk which might occur during treatment by several, even herbal, medications. Many these substances, among them extracts plants, have antimicrobial, anti-inflammatory and immunostimulatory properties owing their polyphenols content, but also may reveal unwanted effects on fetal development anti-angiogenic properties. The aim present study was elucidate whether daily feeding nursing mice 0.2 mg/kg...

Cytokines are important immune modulator factors controlling homeostasis of the body and involved in tissue regeneration after wound healing. The encapsulation cytokines liposomes has many advantages potentially useful for their transfer to cells. Liposomes protect from neutralization, improving pharmacokinetics or biologic activity vivo. They targeted specific cell types may delay release cytokines, allowing sustained paracrine delivery. Their physicochemical characteristics such as size,...

BACKGROUND:Acetylcholinesterase (AChE) and cholinergic receptors have an important role in the immune system angiogenesis. This work evaluated effects of isopropyl methylphosphonofluoridate (IMPF), irreversible inhibitor AChE, on tumor growth selected parameters associated with MATERIAL AND METHODS:Experiments were performed male BALB/c mice exposed to IMPF (study group) or saline buffer (control inoculated L-1 sarcoma; number new blood vessels (TIA test) level αvβ3 integrin (131I-MAb-antiβ3...

W ostatnich latach obserwujemy coraz większe rozpowszechnienie występowania chorób neurodegeneracyjnych na całym świecie. Jest to związane ze wzrostem wieku populacji światowej. Pomimo intensywnych badań naukowych oraz prób klinicznych, w większości przypadków nie opracowano do tej pory skutecznej terapii. Tradycyjne postępowanie przypadku zaburzeń neurologicznych łagodzi jedynie nasilenie objawów bez likwidowania przyczyny choroby. Poszukiwanie nowych metod leczenia jest utrudnione przez...

Age-related macular degeneration (AMD) and diabetic retinopathy are the leading cause of blindness in developed countries. Pathological angiogenesis has a causal role these eye diseases. Resveratrol (RSV), plant-derived polyphenol, anti-proliferative anti-angiogenic properties that could improve its management. Here, effects various concentrations RSV (1, 5, 10, 50, 100 µM) were compared two types cell lines: HECa10 (endothelial line) ARPE-19 (retinal pigment epithelial line). We assayed...