A5000096718- Bone health and osteoporosis research
- Bone Metabolism and Diseases
- Bone and Dental Protein Studies
- Cellular Mechanics and Interactions
- Bone Tissue Engineering Materials
- Bone health and treatments
- Alkaline Phosphatase Research Studies
- Nuclear Physics and Applications
- Bone and Joint Diseases
- Connective tissue disorders research
- Orthopaedic implants and arthroplasty
- Medical Imaging Techniques and Applications
- Calcium Carbonate Crystallization and Inhibition
- X-ray Spectroscopy and Fluorescence Analysis
- Osteoarthritis Treatment and Mechanisms
- Dermatological and Skeletal Disorders
- Trace Elements in Health
- Endodontics and Root Canal Treatments
- Heterotopic Ossification and Related Conditions
- Nuclear Structure and Function
- Orthopedic Infections and Treatments
- Oral microbiology and periodontitis research
- Medical Imaging and Pathology Studies
- Advanced X-ray Imaging Techniques
- Skin and Cellular Biology Research
University of Salzburg
2019-2024
Max Planck Institute of Colloids and Interfaces
2014-2024
Pädagogische Hochschule Salzburg
2021
Ludwig Boltzmann Institute of Osteology
2011-2020
Hanusch Hospital
2011-2020
Max Planck Society
2014-2019
TU Wien
2012-2013
Trace elements are chemical in minute quantities, which known to accumulate the bone. Cortical and trabecular bones consist of bone structural units (BSUs) such as osteons packets different mineral content separated by cement lines. Previous studies investigating trace lacked resolution therefore very little is about local concentration zinc (Zn), strontium (Sr) lead (Pb) BSUs human We used synchrotron radiation induced micro X-ray fluorescence analysis (SR μ-XRF) combination with...
In the present study a rat animal model of lathyrism was employed to decipher whether anatomically confined alterations in collagen cross-links are sufficient influence mechanical properties whole bone. Animal experiments were performed under an ethics committee approved protocol. Sixty-four female (47 day old) rats equivalent weights divided into four groups (16 per group): Controls fed semi-synthetic diet containing 0.6% calcium and phosphorus for 2 or 4 weeks β-APN treated animals...
Significance The explanation of how bone senses and adapts to mechanical stimulation still relies on hypotheses. fluid flow hypothesis claims that a load-induced through the lacunocanalicular network can be sensed by osteocytes, which reside within structure. We show considering architecture results in better prediction remodeling than strain alone. This was done calculating volumes covering complete cross-sections mouse tibiae, underwent controlled vivo loading. established relationship...
Osteocytes interconnect with each other forming an intricate cell network within the mineralized bone matrix. One important function of osteocyte is mechano-regulation remodeling, where a possible mechanism includes fluid flow through porosity housing - lacuno-canalicular (OLCN). In our study OLCN in human osteons was three-dimensionally imaged aim to obtain quantitative description canalicular density and spatial variations this quantity osteons. The topology determined by first staining...
Abstract A popular hypothesis explains the mechanosensitivity of bone due to osteocytes sensing load-induced flow interstitial fluid squeezed through lacunocanalicular network (LCN). However, way in which intricate structure LCN influences is largely unexplored. We therefore aimed quantify real LCNs from human osteons using a combination experimental and computational techniques. Bone samples were stained with rhodamine image 3D confocal microscopy. Image analysis was then performed convert...
Abstract During bone remodeling, osteoblasts are known to deposit unmineralized collagenous tissue (osteoid), which mineralizes after some time lag. Some of the differentiate into osteocytes, forming a cell network within lacunocanalicular (LCN) bone. To get more insight potential role osteocytes in mineralization process osteoid, sites formation three‐dimensionally imaged nine human osteons using focused ion beam‐scanning electron microscopy (FIB‐SEM). In agreement with previous...
Raman microspectroscopy and quantitative backscattered electron imaging (qBEI) of bone are powerful tools to investigate material properties. Both methods provide information on the degree matrix mineralization. However, a head-to-head comparison these outcomes from identical areas has not been performed date. In femoral midshaft cross sections three women, 99 regions (20×20 μm2) were selected inside osteons interstitial covering wide range As focus this study was only undergoing secondary...
ABSTRACT High bone mass in animals and humans with sclerostin deficiency is associated increased strength, which not the case for all disorders high mineral density, some of are even fragility fractures owing to unfavorable composition. In current study we investigated whether alterations composition may contribute strength characteristics lack sclerostin. We examined cortical Sost-knockout (KO) mice (n = 9, 16 weeks old) sclerosteosis patients (young [4 14 years], n 4 adults [24 43 2) by...
ABSTRACT Hypophosphatasia is an inborn error of metabolism caused by mutations in the ALPL gene. It characterized low serum alkaline phosphatase (ALP) activity and defective mineralization bone, but phenotype varies greatly severity depending on degree residual enzyme activity. We describe a man with compound heterozygous ALPL, no previous bone disease, who suffered numerous disabling fractures after he developed progressive renal failure (for which eventually needed dialysis treatment) was...
Abstract Gadolinium-based contrast agents (GBCAs) are frequently used in patients undergoing magnetic resonance imaging. In GBCAs gadolinium (Gd) is present a bound chelated form. Gadolinium rare-earth element, which normally not human body. Though the blood elimination half-life of about 90 minutes, recent studies demonstrated that some tissues retain gadolinium, might further pose health threat due to toxic effects free gadolinium. It known bone tissue can serve as depot, but so far only...
Abstract Little is known about the contribution of 3D surface geometry to development multilayered tissues containing fibrous extracellular matrix components, such as those found in bone. In this study, we elucidate role curvature formation chiral, twisted-plywood-like structures. Tissues consisting murine preosteoblast cells (MC3T3-E1) were grown on scaffolds with constant-mean and negative Gaussian for up 32 days. Using fluorescence microscopy, influence actin stress-fiber alignment...
Osteocyte lacuno-canalicular network (LCN) is comprised of micrometre-sized pores and submicrometric wide channels in bone. Accumulating evidence suggests multiple functions this material transportation, mechanobiological signalling, mineral homeostasis bone remodelling. Combining rhodamine staining confocal laser scanning microscopy, the longitudinal cross-sections six mouse tibiae were imaged, connectome was quantified with a focus on spatial heterogeneities density, connectivity length...
Humanized mouse models are increasingly studied to recapitulate human-like bone physiology. While human and architectures differ in multiple scales, the extent which chimeric human-mouse physiologically interacts structurally integrates remains unknown. Here, we identify that humanized is formed by a mosaic of collagen, integrated within same organ, as shown immunohistochemistry. Combining this with materials science techniques, investigate extracellular matrix specific collagen regions. We...
Abnormal tissue levels of certain trace elements such as zinc (Zn) were reported in various types cancer. Little is known about the role Zn osteosarcoma. Using confocal synchrotron radiation micro X-ray fluorescence analysis, we characterized spatial distribution high-grade sclerosing osteosarcoma nine patients (four women/five men; seven knee/one humerus/one femur) following chemotherapy and wide surgical resection. Levels compared with adjacent normal tissue. Quantitative backscattered...
Various tissue types, including fibrous connective tissue, bone marrow, cartilage, woven and lamellar bone, coexist in healing bone. Similar to most type, contains a lacuno-canalicular network (LCN) housing osteocytes. These cells are known orchestrate remodeling healthy by sensing mechanical strains translating them into biochemical signals. The structure of the LCN is hypothesized influence mineralization processes. Hence, aim present study was visualize match spatial variations topology...
Bone mineral density distributions (BMDDs) are a measurable property of bone tissues that depends strongly on remodelling and mineralisation processes. These processes can vary significantly in health disease across skeletal sites, so there is high interest analysing these from experimental BMDDs. Here, we propose rigorous hypothesis-testing approach based mathematical model heterogeneity due to mineralisation, help explain differences observed between the BMDD human femoral cortical...