A5102940992- Cancer Genomics and Diagnostics
- Bioinformatics and Genomic Networks
- Systemic Lupus Erythematosus Research
- Cancer-related molecular mechanisms research
- Acute Myeloid Leukemia Research
- Gene expression and cancer classification
- RNA modifications and cancer
- Genetic Associations and Epidemiology
- Lipoproteins and Cardiovascular Health
- Computational Drug Discovery Methods
- Ferroptosis and cancer prognosis
- Neuroblastoma Research and Treatments
- MicroRNA in disease regulation
- Lung Cancer Treatments and Mutations
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- Esophageal Cancer Research and Treatment
- Prostate Cancer Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Pancreatic and Hepatic Oncology Research
- Sepsis Diagnosis and Treatment
- Genomic variations and chromosomal abnormalities
- Epigenetics and DNA Methylation
- Atherosclerosis and Cardiovascular Diseases
- Immune cells in cancer
Taichung Veterans General Hospital
2016-2025
National Chung Hsing University
2019-2025
Fu Jen Catholic University
2019-2025
National Taipei University
2024
Institut thématique Génétique, génomique et bioinformatique
2024
National Tsing Hua University
2021-2024
National Yang Ming Chiao Tung University
2023
Academia Sinica
2010-2016
The University of Texas Health Science Center at San Antonio
2011-2014
Children's Cancer Center
2013-2014
Abstract This study utilized Next-Generation Sequencing (NGS) to explore genetic determinants of survival duration in Glioblastoma Multiforme (GBM) patients. We categorized 30 primary GBM patients into two groups based on their periods: extended (over years, N = 17) and abbreviated (under 13). For identifying pathogenic or likely variants, we leveraged the ClinVar database. The cohort, aged 23 66 (median: 53), included 17 Group A (survival >2 10 males, 7 females), 13 B <2 8 5 with a...
Abstract Background ADH1B rs1229984 and ALDH2 rs671 are the specifically prevalent functional variants in East Asians. These variants, which result a dramatic change enzyme activity, highly associated with alcohol‐related disorders cancer. Previous studies focusing on additive synergic effects of few inconsistent. The aim research was to evaluate associations risks disorder Methods This cohort study enrolled 42,665 participants from Taiwan Precision Medicine Initiative database, including...
Abstract Background Although the 2019 EULAR/ACR classification criteria for systemic lupus erythematosus (SLE) has required at least a positive anti-nuclear antibody (ANA) titer (≥ 1:80), it remains challenging clinicians to identify patients with SLE. This study aimed develop machine learning (ML) approach assist in detection of SLE using genomic data and electronic health records. Methods Participants ANA 1:80) were enrolled from Taiwan Precision Medicine Initiative cohort. The Biobank...
Objectives This study aimed to develop a predictive model using polygenic risk score (PRS) forecast renal outcomes for adult systemic lupus erythematosus (SLE) in Taiwanese population. Methods Patients with SLE (n=2782) and matched non-SLE controls (n=11 128) were genotyped Genome-Wide TWB 2.0 single-nucleotide polymorphism (SNP) array. PRS models (C+T, LDpred2, Lassosum, PRSice-2, PRS-continuous shrinkage (CS)) constructed predicting susceptibility. Logistic regression was assessed...
Additional sex combs-like 1 (ASXL1) is frequently mutated in myeloid malignancies. Recent studies showed that hematopoietic-specific deletion of Asxl1 or overexpression mutant ASXL1 resulted myelodysplasia-like disease mice. However, actual effects a "physiological" dose remain unexplored. We established knock-in mouse model bearing the most frequent mutation and studied its pathophysiological on hematopoietic system. Heterozygotes (Asxl1 tm/+ ) marrow cells had higher vitro proliferation...
Abstract The bifunctional enzyme UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE) plays a key role in sialic acid production. It is different from the non-hydrolyzing enzymes for bacterial cell wall biosynthesis and it feed-back inhibited by downstream product CMP-Neu5Ac. Here complex crystal structure of N-terminal epimerase part human GNE shows tetramer which UDP binds to active site CMP-Neu5Ac dimer-dimer interface. locked tightly closed conformation. By comparing UDP-binding modes hydrolyzing...
The standard "7 + 3" induction results in 30% of de novo acute myeloid leukemia (AML) patients not achieving complete remission (CR). We aimed to utilize the Olink® platform compare bone marrow plasma proteomic profiles newly diagnosed AML who did and achieve CR following treatment. This prospective study included 43 untreated patients, stratified into (n = 29) non-CR 14) groups based on their response therapy. employed Explore-384 Inflammation for analysis investigate differences protein...
Thymoma is a rare malignancy with an unclear etiology of occurrence and development. We observed higher incidence thymoma in the Taiwanese population compared to other Western populations, suggesting existence different genomic features. Since most studies are based on we aimed characterize profile compare it TCGA cohort this study. analyzed genome 47 patients using Tumor Mutational Burden Panel discover genetic population. also characterized mutational signatures these samples....
Rationale: Brain metastasis in patients with lung cancer is life-threatening. However, the molecular mechanism for this catastrophic disease remains elusive, and few druggable targets are available. Therefore, study aimed to identify characterize proteins that could be used as therapeutic targets. Methods: Proteomic analyses were conducted differentially expressed membrane between brain metastatic cells primary cells. A neuronal growth-associated protein, acid soluble protein 1 (BASP1), was...
Our previous study demonstrated that myc, mitochondrial oxidative phosphorylation, mTOR, and stemness are independently responsible for chemoresistance in acute myeloid leukemia (AML) cells. This aimed to identify potential mechanisms of the "7 + 3" induction AML by using a single-cell RNA sequencing (scRNA-seq) approach. In present study, 13 untreated patients with de novo were enrolled stratified into two groups: complete remission (CR; n = 8) non-CR (n 5). Single-cell was used analyze...
Large-scale cancer drug sensitivity data have become available for a collection of cell lines, but only limited response from patients are available. Bridging the gap in pharmacogenomics knowledge between vitro and vivo datasets remains challenging. In this study, we trained deep learning model, Scaden-CA, deconvoluting tumor into proportions cancer-type-specific lines. Then, developed prediction method using deconvoluted The Scaden-CA model showed excellent performance terms concordance...
Drug evaluation in tumoroids lacks circulation, limiting infiltration analysis and real-time observation. Using tumor-microenvironment-on-chip (TMoC), this study confirms chemotherapy efficacy mouse patient tumors.
Abstract Background Sepsis is characterized by organ dysfunction as a response to infection and one of the leading causes mortality loss health. The heterogeneous nature sepsis, along with ethnic differences in susceptibility, challenges thorough understanding its etiology. This study aimed propose prediction models leveraging genetic-risk scores clinical variables that can assist risk stratification patients. Methods A total 1,403 patients from Taiwan, diagnosed were utilized. Genome-wide...
Abstract Background Rheumatoid arthritis (RA) and systemic lupus erythematous (SLE) are autoimmune rheumatic diseases that share a complex genetic background common clinical features. This study’s purpose was to construct machine learning (ML) models for the genomic prediction of RA SLE. Methods A total 2,094 patients with 2,190 SLE were enrolled from Taichung Veterans General Hospital cohort Taiwan Precision Medicine Initiative. Genome-wide single nucleotide polymorphism (SNP) data obtained...
Hypertension has been reported as a major risk factor for diseases such cardiovascular disease, and associations between platelet activation hypertension are well-established. However, the exact nature of causality them remains unclear. In this study, bi-directional Mendelian randomization (MR) analysis was conducted on 15,996 healthy Taiwanese individuals aged 30 70 years from Taiwan Biobank, recorded 2008 2015. The inverse variance weighted (IVW) method applied to determine causal...
Abstract Mammographic screening has contributed to a significant reduction in breast cancer mortality. Several studies have highlighted the correlation between density, as detected through mammography, and higher likelihood of developing cancer. A polygenic risk score (PRS) is numerical that calculated based on an individual's genetic information. This study aims explore potential roles PRS candidate markers for development investigate profiles associated with clinical characteristics Asian...
Naja atra is a major venomous snake found in Taiwan. The bite of this causes extensive wound necrosis or necrotizing soft tissue infection. Conventional microbial culture-based techniques may fail to identify potential human pathogens and render antibiotics ineffective the management Therefore, we evaluated 16S Sanger sequencing next-generation (NGS) bacterial species oropharynx N. atra. Using conventional culture methods VITEK 2 system, isolated nine from snakebite wounds. On basis clones...
Abstract Background Sepsis is a frequent complication in critically ill patients, highly heterogeneous and associated with high morbidity mortality rates, especially the elderly population. Utilizing RNA sequencing (RNA-Seq) to analyze biological pathways widely used clinical molecular genetic studies, but studies patients sepsis are still lacking. Hence, we investigated mortality-relevant features transcriptomic who were admitted intensive care unit (ICU) for sepsis. Methods We enrolled 37...