A5102848996- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Circular RNAs in diseases
- RNA Research and Splicing
- Nutrition, Genetics, and Disease
- BRCA gene mutations in cancer
- Connective tissue disorders research
- Thyroid Cancer Diagnosis and Treatment
- Blood groups and transfusion
- Congenital limb and hand anomalies
- Complement system in diseases
- TGF-β signaling in diseases
- Renal Diseases and Glomerulopathies
Medical University of Warsaw
2014-2018
University of Warsaw
2017-2018
The Maria Sklodowska-Curie National Research Institute of Oncology
2013-2014
A single microRNA gene may give rise to several mature products that differ in length, called isomiRs. IsomiRs are known be tissue specific and functionally relevant. The sequence heterogeneity of the thyroid gland has yet determined. objective study was provide a comprehensive view transcriptome normal papillary carcinoma (PTC). We used next-generation deep sequencing analyze length expression profiles PTC tumors (n = 14), unaffected adjacent control, noncancerous 14). results were...
The risk of developing papillary thyroid carcinoma (PTC), the most frequent form malignancy, is elevated up to 8.6‐fold in first‐degree relatives PTC patients. familial could be explained by high‐penetrance mutations yet unidentified genes, or polygenic action low‐penetrance alleles. Since DNA‐damaging exposure ionizing radiation a known factor for cancer, polymorphisms DNA repair genes are likely affect this risk. In search susceptibility alleles we employed Sequenom technology genotype...
Both obesity and weight loss may cause molecular changes in adipose tissue. This study aimed to characterize tissue miRNome order identify pathways affected by changes. Next generation sequencing (NGS) was applied microRNAs (miRNAs) differentially expressed 47 samples of visceral (VAT) subcutaneous (SAT) tissues from normal-weight (N), obese (O) after surgery-induced (PO) individuals. Subsequently miRNA expression validated real-time PCR 197 bioinformatics analysis performed obesity-related...
// Łukasz Koperski 1 , Marta Kotlarek 2 Michał Świerniak 2, 3 Monika Kolanowska Anna Kubiak Barbara Górnicka Krystian Jażdżewski and Wójcicka Department of Pathology, Medical University Warsaw, Poland Laboratory Human Cancer Genetics, Center New Technologies, CENT, Genomic Medicine, Correspondence to: Wójcicka, email: anna.wojcicka@uw.edu.pl Keywords: microRNA, adrenocortical carcinoma, cancer diagnostics, next-generation sequencing, NGS Abbreviations: AA, adenoma; ACC, carcinoma; NA, normal...
Aberrant expression of the sodium-iodide symporter (NIS) and resistance to post-operative radioactive iodide treatment is a crucial cause higher mortality some thyroid cancer patients. In this study, we analyzed impact miR-146a on function NIS overall survival The study included 2441 patients (2163 women; 278 men); including 359 cases with follicular variant papillary carcinoma (fvPTC). miR:NIS interactions were in cell lines using vivo binding inhibition assays iodine uptake assays....
MicroRNAs, non-coding regulators of gene expression, are known culprits thyroid cancer. Using next-generation sequencing, we identified a novel microRNA gene, encoded within an important regulator - thyroglobulin, and analyzed its functionality in the gland. In vitro silico analyses proved that miR-TG is processed from precursor, co-expressed with thyroglobulin. Both genes specific for tissue downregulated papillary carcinoma by 44% (p = 0.04) 48% 0.001), respectively. Putative target were...
Abstract Background Brachydactylies are a group of inherited conditions, characterized mainly by the presence shortened fingers and toes. Based on patients’ phenotypes, brachydactylies have been subdivided into 10 subtypes. In this study, we identified family with two members affected brachydactyly type A2 (BDA2). BDA2 is caused mutations in three genes: BMPR1B, BMP2 or GDF5. So far only studies reported cases BMPR1B gene. Methods We employed next‐generation sequencing to identify culpable...
Atypical hemolytic uremic syndrome (aHUS) is a rare disease triggered by dysregulation of the alternative complement pathway, consisting characteristic triad nonimmune anemia, thrombocytopenia, and renal failure. The risk aHUS onset, recurrence, allograft loss depends on genetic background patient. We show series cases from single family whose five members were affected presented distinct clinical outcomes. Next-generation sequencing revealed combined mutations in both factor H membrane...
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)