The structures of membrane receptors mediating rapid, nongenomic actions steroids have not been identified. We describe the cloning a cDNA from spotted seatrout ovaries encoding protein that satisfies following seven criteria for its designation as steroid receptor: plausible structure, tissue specificity, cellular distribution, binding, signal transduction, hormonal regulation, and biological relevance. For computer modeling predicts has transmembrane domains, typical G protein-coupled...

Recently we discovered a previously uncharacterized gene with the characteristics of membrane progestin receptor (mPR) in fish model, spotted seatrout. Here, report identification, cloning, and other members this hitherto unknown family putative mPRs from several vertebrate species, including human, mouse, pig, Xenopus , zebrafish, Fugu highly conserved nucleotide deduced amino acid sequences similar structures to seatrout mPR. The 13 genes identified seem belong an family. Phylogenetic...

A novel progestin receptor (mPR) with seven-transmembrane domains was recently discovered in spotted seatrout and homologous genes were identified other vertebrates. We show that cDNAs for the mPR alpha subtypes from (st-mPRalpha) humans (hu-mPRalpha) encode receptors display many functional characteristics of G protein-coupled receptors. Flow cytometry immunocytochemical staining whole MDA-MB-231 cells stably transfected mPRalphas using antibodies directed against their N-terminal regions...

Public concerns on phthalates distributions in the environment have been increasing since they can cause liver cancer, structural abnormalities and reduce sperm counts male reproductive system. However, few data are actually available effects of Di-(2-ethylhexyl)-phthalate (DEHP) female The aim this study was to assess impacts DEHP zebrafish oogenesis embryo production. Female Danio rerio were exposed environmentally relevant doses a significant decrease ovulation production observed....

As sessile organisms, plants have evolved a wide range of defence pathways to cope with environmental stress such as heat shock. However, the molecular mechanism these remains unclear in rice. In this study, we found that OsHSFA2d, shock transcriptional factor, encodes two main splice variant proteins, OsHSFA2dI and OsHSFA2dII Under normal conditions, is dominant but transcriptionally inactive spliced form. when plant suffers stress, OsHSFA2d alternatively into active form, OsHSFA2dI, which...

Fanconi anemia (FA) is an autosomal recessive disease characterized by congenital anomalies, aplastic anemia, and chromosomal instability. A cDNA encoding the FA complementation group C (FACC) polypeptide was recently cloned [Strathdee, C. A., Gavish, H., Shannon, W. R. & Buchwald, M. (1992) Nature (London) 356, 763-767]. To further characterize this polypeptide, we generated a rabbit polyclonal antiserum against its carboxyl terminus. We used to analyze FACC from normal or mutant...

The zebrafish nuclear progestin receptor (nPR; official symbol PGR) was identified and characterized to better understand its role in regulating reproduction this well-established teleost model. A full-length cDNA that encoded a 617-amino acid residue protein with high homology PGRs other vertebrates, contained five domains characteristic of steroid receptors. In contrast the multiplicity receptors often found euteleosts attributed probable genome duplication, only single locus encoding pgr...

Progestins, progesterone derivatives, are the most critical signaling steroid for initiating final oocyte maturation (FOM) and ovulation, in order to advance fully-grown immature oocytes become fertilizable eggs basal vertebrates. It is well-established that progestin induces FOM at least partly through a membrane receptor non-genomic process, which precedes triggered ovulation mediated nuclear (Pgr) genomic pathway. To determine whether Pgr plays role mechanism during FOM, we knocked out...

Recently, a unique family of membrane progestin receptors (mPRalpha, mPRbeta, and mPRgamma) was identified, which may be responsible for mediating rapid, nongenomic actions progestins in variety target tissues. In this study, the mPRalpha mPRbeta isoforms from zebrafish were shown to rapidly specifically activated by maturation-inducing steroid (MIS) species, 4-pregnen-17,20beta-diol-3-one (17,20beta-DHP). The previously uncharacterized isoform stably expressed nuclear progesterone...

Hypoimmunity and numerous stresses are two major challenges in broiler industry. Nutrient intervention at the specific time of embryonic stage is a feasible way to improve animal performance. This study was conducted investigate possible effects ovo feeding (IOF) vitamin C age 15th day (E15) on growth performance, antioxidation immune function broilers. A total 240 fertile eggs were randomly divided into groups (0 3 mg injected dose E15), new-hatched chicks from each treatment allocated six...

Recent investigations suggest progestin receptor membrane component 1 (PGRMC1) associates with and transports a wide range of molecules such as heme, cytochromes P450, steroids 21 carbons, alpha (mPRα/Paqr7), epidermal growth factor (EGFR), insulin receptor. It is difficult to discriminate the true functions PGRMC1 from its associated using biochemical pharmacological approaches. To determine physiological function(s) PGRMC1, we generated global knockouts for pgrmc1 (pgrmc1-/-) in zebrafish....

Somatolactin (SL) is a pituitary hormone belonging to the growth hormone/prolactin superfamily, with recognizable homologues in all fish taxa examined date. Although sequences from most share reasonably high sequence identity, several more highly divergent SLs have been reported. Goldfish SL and second protein found rainbow trout (rtSLP) are remarkably different each other also dissimilar SLs. It has unclear whether rtSLP recent paralogue restricted trout, or reflects ancient duplication of...

Ovulation requires proteinases to promote the rupture of ovarian follicles. However, identity these remains unclear. In our previous studies using RNA-seq analysis differential expressed genes, we found significant down-regulation five metalloproteinases: adam8b (a disintegrin and metalloproteinase domain 8b), adamts8a with thrombospondin motif 8a), adamts9, mmp2 (matrix 2), mmp9 in nuclear progestin receptor knockout (pgr−/−) zebrafish that have failed ovulate. We hypothesize...

Elucidating the genes that contribute to behavioral variation has become an important endeavor in studies. While advances genomics have narrowed down candidate genes, functionally validating them been lagging, partly because of challenges associated with rapid gene manipulations. Consequently, few studies demonstrated causal genetic changes linked behaviors. The ‘gene editing revolution’ offered unprecedented opportunities interrogate responsible for critical Here, we edited androgen...

In rodents, there is increasing evidence that nuclear progesterone receptors are transiently expressed in many regions of the developing brain, notably outside hypothalamus. This suggests and/or its metabolites could be involved functions not related to reproduction, particularly neurodevelopment. this context, adult fish brain particular interest, as it exhibits constant growth and high neurogenic activity supported by radial glia progenitors. However, although synthesis neuroprogestagens...