Abstract The anticancer effects of ceramide have been reported in many types cancers but less lung cancer. In this study, we used C 2 -ceramide to further investigate its possible and mechanisms on non-small cell cancer (NSCLC) H1299 cells. result proliferation terms trypan blue assay showed high dose inhibited survival after 24 h treatment. flow cytometry-based assays indicated the effect apoptosis, chromatin condensation, G 1 arrest Annexin V/propidium iodide (PI), DAPI, PI stainings,...

To compare the prognostic performance between different comorbidity assessments of survival in patients with operated lung cancer. A total 4508 cancer treated by surgery 2003 and 2012 were identified through Taiwan's National Health Insurance Research Database. Information on pre-existing comorbidities prior to diagnosis was obtained adapted Charlson index, age-adjusted index (ACCI) Elixhauser scores. The influence analysed using a Cox proportional hazard model. discriminatory ability...

Caffeic acid (CA), a natural phenolic compound, is abundant in medicinal plants. CA possesses multiple biological effects such as anti-bacterial and anti-cancer growth. was also reported to induce fore stomach kidney tumors mouse model. Here we used two human lung cancer cell lines, A549 H1299, clarify the role of proliferation. The growth assay showed that moderately promoted proliferation cells. Furthermore, pre-treatment rescues inhibition induced by sub-IC50 dose paclitaxel (PTX), an...

Trans-ferulic (FA) acid exhibits antioxidant effects in vitro. However, the underlying mechanism of trans-FA activity cellular physiology, especially cancer remains largely unknown. This study investigated physiological on H1299 human lung cell line. The 2,2-diphenyl-1-picrylhydrazyl assay was used to determine free radical scavenging capability. Assessment intracellular reactive oxygen species (ROS) evaluated using oxidized 2ʹ,7ʹ-dichlorofluorescin diacetate and dihydroethidium staining....

Sirtuins, NAD + -dependent deacetylases, could target both histones and nonhistone proteins in mammalian cells. Sirt1 is the major sirtuin has been shown to involve various cellular processes, including antiapoptosis, senescence. was reported be overexpressed many cancers, lung cancer. Sirtinol, a specific inhibitor of Sirt1, induce apoptosis cancer cells by elevating endogenous level reactive oxygen species. In study, we investigated effect sirtinol on proliferation nonsmall cell (NSCLC)...

Ceramides, abundant sphingolipids on the cell membrane, can act as signaling molecules to regulate cellular functions including viability. Exogenous ceramide has been shown exert potent anti-proliferative effects against cancer cells, but little is known about how it affects reactive oxygen species (ROS) in lung cells. In this study, we investigated effect of N-octanoyl-D-erythro-sphingosine (C₈-ceramide) human non-small-cell H1299 Flow cytometry-based assays indicated that C₈-ceramide...

Early detection of oral malignant or precancerous lesion by screening individuals with high-risk factors may identify candidates who should receive treatment to prevent cancer progression and reduce patient mortality. Among the diagnostic tools, in vivo staining is advocated as a simple, inexpensive, fairly sensitive method.The present study involved examination 58 patients suspected having lesions methylene blue staining. The results uptake were compared simultaneous biopsy these lesions....

2,9-Bis[2-(pyrrolidin-1-yl)ethoxy]-6-{4-[2-(pyrrolidin-1-yl)ethoxy] phenyl}-11H-indeno[1,2-c]quinolin-11-one (BPIQ) is a derivative from 6-arylindeno[1,2-c]quinoline. Our previous study showed the anti-cancer potential of BPIQ compared to its two analogues topotecan and irinotecan. In study, aim investigate potency mechanism against lung cancer cells. Both in vitro zebrafish xenograft model were performed examine anti-lung effect BPIQ. Flow cytometer-based assays for detecting apoptosis cell...

This study was undertaken to investigate the facial skeletal features associated with chin deviation.Twenty-five patients (14 males and 11 females) diagnosed deviations of more than 2 mm were recruited. Fifteen landmarks, including median lateral points, located on posteroanterior cephalograms. The CG-ANS (crista-galli ethmoid-anterior nasal spine) line perpendicular through CG used as references. differences between distances from paired points reference lines examined intraclass...

Abstract Background: Publications regarding the 100 top-cited articles in a given discipline are common, but studies reporting association between article topics and their citations lacking. Whether or not reviews original have higher impact factor than case reports is point for verification this study. In addition, that can be used predicting been analyzed. Thus, study aims to Methods: We searched PubMed Central downloaded abstracts journal Medicine (Baltimore) since 2011. Four types 7...

Her2/neu was thought to be a proto-oncogene with sequence homology epidermal growth factor receptor (EGFR). Its overexpression seen in many cancers and referred regimens of anticancer therapy. The aim this study investigate whether the abnormal expression existed oral carcinogenesis.Immunohistochemistry (IHC) used detect normal mucosa (NOM) (n = 20), precancerous lesions epithelial dysplasia (ED) squamous cell carcinoma (OSCC) 30). association clinicopathologic covariates areca use, tumor...

2,9-Bis[2-(pyrrolidin-1-yl)ethoxy]-6-{4-[2-(pyrrolidin-1-yl)ethoxy] phenyl}-11H-indeno[1,2-c]quinoline-11-one (BPIQ), is a synthetic quinoline analog. A previous study showed the anti-cancer potential of BPIQ through modulating mitochondrial-mediated apoptosis. However, effect on cell migration, an index cancer metastasis, has not yet been examined. Furthermore, among signal pathways involved in stresses, members mitogen-activated protein kinase (MAPK) family are crucial for regulating...

Ceramide is a sphingolipid which regulates variety of signaling pathways in eukaryotic cells. Exogenous ceramide has been shown to induce cellular apoptosis. In this study, we observed that exogenous induced two distinct morphologies cell fate following C2-ceramide treatment between the breast cancer lines MCF-7 (wild type p53) and MDA-MB-231 (mutant The growth assessment showed caused significant inhibition apoptosis cells through down-regulating expression mutant p53 whereas up-regulating...

ABSTRACT Background and objective: Epidermal growth factor receptor ( EGFR ) mutations are common in lung adenocarcinomas of Asian patients, implying a good response to treatment with the tyrosine kinase inhibitors, gefitinib erlotinib. However, distinct chromosomal imbalances between those without have not been fully elucidated. Methods: Seventy‐seven patients surgically resected adenocarcinoma were analysed for exon 19 deletion L858R mutation, using mutant‐enriched PCR, imbalance...

Nanotitanium dioxide particle (nTiO 2 ) inhalation has been reported to induce lung parenchymal injury. After of nTiO , we monitored changes in 5-lipoxygenase, endothelial nitric oxide synthase (eNOS), and inducible (iNOS) mRNA rat tissue. Lung function parameters include specific airway resistance (SRaw), peak expiratory flow rate (PEF), functional residual capacity (FRC), compliance (Cchord); blood white cell count (WBC), (NO), hydrogen peroxide, lactic dehydrogenase (LDH); lavage...

In recent years, combination chemotherapy is a primary strategy for treating lung cancer; however, the issues of antagonism and side effects still limit its applications. The development chemosensitizer aims to sensitize chemoresistant cancer cells anticancer drugs therefore improve efficacy chemotherapy. this study, we examined whether N‐[2‐(morpholin‐4‐yl)phenyl]‐2‐{8‐oxatricyclo[7.4.0.0,2,7]trideca‐1(9),2(7),3,5,10,12‐hexaen‐4‐yloxy}acetamide (NPOA), an acetamide derivative, sensitizes...

CCAAT enhancer-binding proteins (CEBPs) play key roles in the metabolic regulation, cell transformation, and inflammation. However, expression and/or functions of CEBPs rats with hyperglycemia are still unclear. In present study, we investigated changes protein lung diabetic rats. The levels C/EBPβ C/EBPδ were decreased isolated from streptozotocin-induced (STZ-diabetic rats) as compared that normal Exogenous insulin at dose sufficient to normalize plasma glucose STZ-diabetic reversed after...

Background: Hepatocellular carcinoma (HCC) is one of the leading cancers in world, including Taiwan. The chemoresistance advanced HCC frequently results poor prognosis patients. Previous studies demonstrated quinoline derivative, 9-bis[2-(pyrrolidin-1-yl)ethoxy]-6-{4-[2-(pyrrolidin-1-yl)ethoxy]phenyl}-11Hindeno[ 1,2-c]quinolin-11-one (BPIQ) exerts inhibitory potential against several cancer cells, liver cells. Keywords: BPIQ, quinoline, HCC, DNA damage, apoptosis, ER stress, survivin, XIAP, γH2AX.