Deficiency of the membrane protein FAT/CD36 causes a marked defect in fatty acid uptake by various tissues and is genetically linked to insulin resistance rats humans. Here, we examined responsiveness CD36–/– mice. When fed diet high complex carbohydrates low (5%) fat, these animals cleared glucose faster than wild-type. In vivo, 2-fluorodeoxyglucose muscle was increased severalfold, vitro, glycogenesis soleus enhanced. Null mice had lower glycogen levels liver, triglyceride levels, liver...

Deficiency of the membrane protein FAT/CD36 causes a marked defect in fatty acid uptake by various tissues and is genetically linked to insulin resistance rats humans. Here, we examined responsiveness CD36–/– mice. When fed diet high complex carbohydrates low (5%) fat, these animals cleared glucose faster than wild-type. In vivo, 2-fluorodeoxyglucose muscle was increased severalfold, vitro, glycogenesis soleus enhanced. Null mice had lower glycogen levels liver, triglyceride levels, liver...

Abstract Quantifying corneal elasticity after femtosecond laser‐assisted in situ keratomileusis (FS‐LASIK) procedure plays an important role improving surgical safety and quality, since some latent complications may occur ascribing to changes postoperative biomechanics. Nevertheless, it is suggested that current research has been severely constrained due the lack of accurate quantification method obtain distribution. In this paper, acoustic radiation force optical coherence elastography...

The plasma membrane fatty acid transport protein FAT/CD36 is also present at the mitochondria, where it may contribute to regulation of oxidation, although this has been challenged. Therefore, we have compared enzyme activities and rates mitochondrial palmitate oxidation in muscles wild-type (WT) knockout (KO) mice, rest after muscle contraction. In WT KO carnitine palmitoyltransferase-I, citrate synthase, beta-hydroxyacyl-CoA dehydrogenase did not differ subsarcolemmal (SS)...

Muscle contains various fatty acid transporters (CD36, FABPpm, FATP1, FATP4). Physiological stimuli (insulin, contraction) induce the translocation of all four to sarcolemma enhance uptake similarly glucose stimulation via transporter‐4 (GLUT4) translocation. Akt2 mediates insulin‐induced, but not contraction‐induced, GLUT4 translocation, its role in muscle transporter is unknown. In from Akt2‐knockout mice, we observed that critically involved both insulin‐induced and contraction‐induced...

It has been assumed that the uptake of long chain fatty acids (LCFAs) into skeletal muscle and heart muscle, as well other tissues, occurred via passive diffusion. In recent years our work shown LCFA is a highly regulated process. The use giant sarcolemmal vesicles obtained from used to demonstrate these tissues occurs protein-mediated mechanism involving 40 kDa plasma membrane associated acid binding protein (FABPpm) 88 translocase, homologue human CD36 (FAT/CD36). Both are ubiquitously...

Careful quantification of the changes in biomechanical properties iris can offer insight into pathophysiology some ocular diseases. However, to date there has not been much information available regarding this subject because clinical detection for elasticity remains challenging. To overcome limitation, we explore, first time our knowledge, potential measuring using acoustic radiation force optical coherence elastography (ARF-OCE). The resulting images and shear wave propagation, as well...

Evaluating biomechanical properties of trabecular meshwork (TM) is great significance for understanding the mechanism aqueous humor circulation and its relationship to some eye diseases such as glaucoma; however, there almost no relevant study due lack clinical measurement tool. In this paper, an acoustic radiation force optical coherence elastography (ARF-OCE) system developed with advantages noninvasive detection, high resolution, sensitivity, high-speed imaging, by which elastic modulus...

Biomechanical properties of corneal scar are strongly correlated with many diseases and some types surgery, however, there is no elasticity information available about to date. Here, we proposed an acoustic radiation force optical coherence elastography system evaluate elasticity. Elasticity quantification was first conducted on ex vivo rabbit corneas, the results validate efficacy our system. Then, experiments were performed human scarred cornea, where structural features, elastic wave...

Chronic kidney disease (CKD) and diabetic retinopathy (DR) are two serious complications of diabetes. However, the association between retinal neurodegeneration in DR renal function decline is still unclear. Our objective was to evaluate by measure estimated glomerular filtration rate (eGFR), macular ganglion cell-inner plexiform layer (GC-IPL), cell complex (GCC) thickness patients with type 2 diabetes mellitus (T2DM).We analyzed baseline data Guangzhou Diabetic Eye Study. T2DM from...

Purpose: Quantification of biomechanical properties keratoconus (KC) corneas has great significance for early diagnosis and treatment KC, but the corresponding clinical measurement remains challenging. Here, we developed an acoustic radiation force (ARF) optical coherence elastography technique explored its potential evaluating KC corneas. Methods: An ARF system was used to induce tissue deformation, which detected by tomography system, thus localized point-by-point Young's modulus...

To evaluate iris volume before and after pupil dilation using swept-source anterior segment optical coherence tomography (SS-ASOCT) investigate the associated factors of change in patients with type 2 diabetes mellitus (T2DM).A cross-sectional study was conducted Zhongshan Ophthalmic Center among T2DM registered community Guangzhou, China. Anterior chamber (ACV), volume, depth (ACD), angle opening distance at 500 μm (AOD 500) diameter were estimated SS-ASOCT (CASIA; Tomey, Nagoya, Japan)....

Despite the rapidly growing popularity of laser vision correction (LVC) in myopia, its quantitative evaluation has not been thoroughly investigated. In this paper, an acoustic radiation force–optical coherence elastography (ARF-OCE) system was proposed to evaluate LVC by measuring residual stromal bed (RSB) elasticity, because it is directly relevant RSB thickness that critical maintaining normal corneal function. As expected, Young’s modulus calculated, then relationship with determined....

AIM: To investigate the proportion and characteristic of emmetropia in schoolchildren aged 6-11, especially estimate normal value ocular biometric parameters emmetropia. METHODS: A population-based cross-sectional study was conducted on children 6-11y Shenzhen. Totally, 2386 from two primary schools were involved. The axial length (AL) corneal radius curvature (CRC) measured by partial coherence laser interferometry. Noncycloplegic refraction refractive astigmatism (RA) using autorefraction....

The purpose of this paper is to identify the characteristic endoscopic findings in patients with esophageal cavernous lymphangioma and assess efficacy techniques management disease. We retrospectively analyzed data from 6 who were diagnosed by endoscopy histologic evaluation. All underwent resection tumor at our hospital between January 2010 June 2011. Four male 2 female patients, a mean age 48.2±15.2 years (range, 35 77 y) lymphangioma, followed included report. lesions varied 0.4 1.2 cm...

The scleral elasticity is closely related with many ocular diseases, but the relevant research still insufficient. Here, we utilized optical coherence elastography to carefully study biomechanical properties of sclera at different positions and under intraocular pressures. Meanwhile, elastic wave velocity Young's modulus each position were obtained using a phase algorithm. Accordingly, two-dimensional distribution image was achieved by mapping values corresponding structure based on...

SignificancePrevious studies have demonstrated that the biomechanical properties of optic nerve head (ONH) are associated with a variety ophthalmic diseases; however, they not been adequately studied.AimWe aimed to obtain two-dimensional (2D) velocity distribution image based on one-to-one correspondence between values and position using acoustic radiation force optical coherence elastography (ARF-OCE) technique combined 2D phase algorithm.ApproachAn ARF-OCE system has advantages...