A5101625962- Force Microscopy Techniques and Applications
- Biochemical and Structural Characterization
- Advanced Sensor and Energy Harvesting Materials
- Molecular Junctions and Nanostructures
- Advanced Materials and Mechanics
- Electrospun Nanofibers in Biomedical Applications
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Lipid Membrane Structure and Behavior
- Wetland Management and Conservation
- Monoclonal and Polyclonal Antibodies Research
- Nanocluster Synthesis and Applications
- Advanced Electron Microscopy Techniques and Applications
- Carbon dioxide utilization in catalysis
- Fluoride Effects and Removal
- Metalloenzymes and iron-sulfur proteins
- CO2 Reduction Techniques and Catalysts
- DNA and Nucleic Acid Chemistry
- Mechanical and Optical Resonators
- Iron Metabolism and Disorders
- Dielectric materials and actuators
- Peptidase Inhibition and Analysis
- Adsorption and biosorption for pollutant removal
- Advanced Biosensing Techniques and Applications
- Magnetism in coordination complexes
Nanjing University
2019-2023
Collaborative Innovation Center of Advanced Microstructures
2019
China Astronaut Research and Training Center
2017
Abstract There is often a trade-off between mechanical properties (modulus and toughness) dynamic self-healing. Here we report the design synthesis of polymer containing thermodynamically stable whilst kinetically labile coordination complex to address this conundrum. The Zn-Hbimcp (Hbimcp = 2,6-bis((imino)methyl)-4-chlorophenol) bond used in work has relatively large association constant (2.2 × 10 11 ) but also undergoes fast reversible intra- inter-molecular ligand exchange processes....
Self-sensing actuators are critical to artificial robots with biomimetic proprio-/exteroception properties of biological neuromuscular systems. Existing add-on approaches, which physically blend heterogeneous sensor/actuator components, fall short yielding satisfactory solutions, considering their suboptimal interfaces, poor adhesion, and electronic/mechanical property mismatches. Here, a single homogeneous material platform is reported by creating silver-polymer framework (SPF), thus...
The recent development of chemical and bio-conjugation techniques allows for the engineering various protein polymers. However, most polymerization process is difficult to control. To meet this challenge, we develop an enzymatic procedure build polyprotein using combination a strict ligase OaAEP1 (Oldenlandia affinis asparaginyl endopeptidases 1) protease TEV (tobacco etch virus). We firstly demonstrate use OaAEP1-alone sequence-uncontrolled ubiquitin covalently immobilize coupled on...
Open AccessCCS ChemistryRESEARCH ARTICLE1 Feb 2022Combination of Click Chemistry and Enzymatic Ligation for Stable Efficient Protein Immobilization Single-Molecule Force Spectroscopy Shengchao Shi, Ziyi Wang, Yibing Deng, Fang Tian, Qingsong Wu Peng Zheng Shi State Key Laboratory Coordination Chemistry, Biomedicine Innovation Center (ChemBIC), School Chemical Engineering, Nanjing University, 210023 , Wang Deng Tian *Corresponding author: E-mail Address: [email protected]...
SMFS and MD simulations revealed a closed conformation decreased stability of sortase-mediated polyprotein I27 when linker with high content glycine is used.
Enzymes are widely used for protein ligation because of their efficient and site-specific connections under mild conditions. However, many enzymatic ligations restricted to between termini while protein-protein conjugation at a specific internal site is limited. Previous work has found that Sortase A (SrtA) conjugates small molecules/peptides pilin an lysine via isopeptide bond. Herein, we apply this noncanonical property SrtA designed YPKH site. Both domain, I27, large protein, GFP, were...
We report the use of a protein ligase to covalently ligate peptide nucleic acid (PNA). The rapid ligation demands only an N-terminal GL dipeptide in target and C-terminal NGL tripeptide PNA. demonstrate versatility this approach by attaching PNA strand three different proteins. Lastly, we show that erasable imaging EGFR on HEK293 cell membranes is achieved with DNA origami nanostructures toehold-mediated displacement.
Human metallothionein (MT) is a small-size yet efficient metal-binding protein, playing an essential role in metal homeostasis and heavy detoxification. MT contains two domains, each forming polynuclear cluster with exquisite hexatomic ring structure. The apoprotein intrinsically disordered, which may strongly influence the clusters metal-thiolate (M-S) bonds, leading to highly dynamic However, these features are challenging identify due transient nature of species. individual signal from...
Chemical and bio-conjugation techniques have been developed rapidly in recent years allow the building of protein polymers. However, a controlled polymerization process is always challenge. Here, we an enzymatic methodology for constructing polymerized step by rationally-controlled sequence. In this method, C-terminus monomer NGL conjugation using OaAEP1 (Oldenlandia affinis asparaginyl endopeptidases) 1) while N-terminus was cleavable TEV (tobacco etch virus) cleavage site plus L...
Enzymatic protein ligation has become the most powerful and widely used method for high-precision atomic force microscopy-based single-molecule spectroscopy (AFM-SMFS) study of mechanics.
The outer mitochondrial membrane protein mitoNEET (mNT) is a recently identified iron-sulfur containing unique Fe2 S2 (His)1 (Cys)3 metal cluster with single Fe-N(His87) coordinating bond. This labile Fe-N bond led to multiple unfolding/rupture pathways of mNT and its by atomic force microscopy-based single-molecule spectroscopy (AFM-SMFS), one most common tools for characterizing the molecular mechanics. Although previous ensemble studies showed that this Fe-N(His) essential function, they...
Chemical and bio-conjugation techniques have been developed rapidly in recent years allow the building of protein polymers. However, a controlled polymerization process is always challenge. Here, we an enzymatic methodology for constructing polymerized step by rationally-controlled sequence. In this method, C-terminus monomer NGL conjugation using OaAEP1 (Oldenlandia affinis asparaginyl endopeptidases) 1) while N-terminus was cleavable TEV (tobacco etch virus) cleavage site plus L...
The development of chemical and biological coupling technologies in recent years has made possible protein polymers engineering.We have developed an enzymatic method for building polyproteins using a ligase OaAEP1 (asparagine endopeptidase 1) protease TEV (tobacco etching virus).Using mobile site compatible with the ligation, we achieved stepwise polymerization on surface.The produced polyprotein can be verified by unfolding scenario atomic force microscopy-based single-molecule spectroscopy...
Abstract Sensorized actuators are critical to imitate proprio-/exteroception properties of biological neuromuscular systems. Existing add-on approaches, which physically blend heterogeneous sensor/actuator components, fall short yielding satisfactory solutions, considering their suboptimal interfaces, poor adhesion, and electronic/mechanical property mismatch. Here, we report a single homogeneous material comprising seamless sensing-actuation unification at nano-/molecule levels, in built-in...
Abstract Methods to efficiently and site-specifically conjugate proteins nucleic acids could enable exciting application in bioanalytics biotechnology. Here, we report the use of strict protein ligase covalently ligate a peptide acid (PNA). The rapid ligation requires only short N-terminal GL dipeptide target C-terminal NGL tripeptide PNA. We demonstrate versatility this approach by conjugating three different types with PNA strand. biostable strand then serves as generic landing platform...