A5046190479- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Pluripotent Stem Cells Research
- Electrospun Nanofibers in Biomedical Applications
- Congenital heart defects research
- Adipose Tissue and Metabolism
- Cardiovascular Function and Risk Factors
- Nitric Oxide and Endothelin Effects
- Birth, Development, and Health
- Pregnancy and preeclampsia studies
- Cardiac Structural Anomalies and Repair
- Animal Genetics and Reproduction
- Chromosomal and Genetic Variations
- Cardiac Fibrosis and Remodeling
- Cancer, Hypoxia, and Metabolism
- Growth Hormone and Insulin-like Growth Factors
- Cardiac Ischemia and Reperfusion
- CRISPR and Genetic Engineering
- Mitochondrial Function and Pathology
- Metabolism, Diabetes, and Cancer
- Neuropeptides and Animal Physiology
- Muscle Physiology and Disorders
- Bone Metabolism and Diseases
- Cardiovascular Disease and Adiposity
- Reproductive Biology and Fertility
University of Miami
2016-2025
Stem Cell Institute
2011-2023
Midwest Institute for Minimally Invasive Therapies
2020-2022
Miami Transplant Institute
2012-2018
Centro de Investigación en Red en Enfermedades Cardiovasculares
2018
Centro de Investigación Biomédica en Red
2018
Medical University of Vienna
2018
Instituto de Salud Carlos III
2018
Hospital General Universitario Gregorio Marañón
2018
San Diego State University
2017
Endothelial dysfunction, characterized by diminished endothelial progenitor cell (EPC) function and flow-mediated vasodilation (FMD), is a clinically significant feature of heart failure (HF). Mesenchymal stem cells (MSCs), which have pro-angiogenic properties, the potential to restore function. Accordingly, we tested hypothesis that MSCs increase EPC (FMD).Idiopathic dilated ischemic cardiomyopathy patients were randomly assigned receive autologous (n = 7) or allogeneic 15) MSCs. We...
Although mammalian cardiac regeneration can occur in the neonatal period, factors involved this process remain to be established. Because tissue and limb require concurrent reinnervation by peripheral nervous system, we hypothesized that also requires reinnervation.To test hypothesis is required for innate regeneration.We crossed a Wnt1-Cre transgenic mouse with double-tandem Tomato reporter strain identify neural crest-derived cell lineages including autonomic nerves heart. This approach...
Significance A high-resolution genetic lineage-tracing study in mice reveals that cKit identifies multipotent progenitors of cardiac neural crest (CNC) origin. Normally, the proportion cardiomyocytes produced from this lineage is limited, not because poor differentiation capacity as previously thought, but stage-specific changes activity bone morphogenetic protein pathway. Transient antagonism efficiently directs mouse iPSCs toward CNC and, consequently, generation + CNCs with full to form...
Retinoic acid (RA) receptors (RARs) are ligand-inducible transcription factors that bind to specific DNA sequences associated with the regulatory regions of RA-regulatable genes. Since RA has been implicated as an important factor both in normal development and teratological studies, one would like have a model system detects presence activated during development. We constructed recombinant reporter gene three copies response element (RARE) from RAR beta-2 promoter 5' herpes simplex virus...
Although nitric oxide (NO) signaling promotes differentiation and maturation of endothelial progenitor cells, its role in the mesenchymal stem cells (MSCs) into remains controversial. We tested NO MSCs derived from WT mice homozygous for a deletion S -nitrosoglutathione reductase (GSNOR −/− ), denitrosylase that regulates -nitrosylation. GSNOR exhibited markedly diminished capacity vasculogenesis an vitro Matrigel tube–forming assay vivo relative to MSCs. This decrease was associated with...
Abstract Whereas cardiac-derived c-kit+ stem cells (CSCs) and bone marrow-derived mesenchymal (MSCs) are undergoing clinical trials testing safety efficacy as a cell-based therapy, the relative therapeutic biologic of these two cell types is unknown. We hypothesized that human CSCs have greater ability than MSCs to engraft, differentiate, improve cardiac function. compared intramyocardial injection fetal (36,000) with doses adult (36,000 1,000,000) or control (phosphate buffered saline) in...
Culture-expanded cells originating from cardiac tissue that express the cell surface receptor cKit are undergoing clinical testing as a source for heart failure and congenital disease. Although accumulating data support mesenchymal stem (MSCs) enhance efficacy of cKit(+) (CSCs), underlying mechanism this synergistic effect remains incompletely understood.To test hypothesis MSCs stimulate endogenous CSCs to proliferate, migrate, differentiate via SDF1/CXCR4 factor/cKit pathways.Using genetic...
Bone marrow-derived mesenchymal stem cells (MSCs) are a common precursor of both adipocytes and osteoblasts. While it is appreciated that PPARγ regulates the balance between adipogenesis osteogenesis, roles additional regulators this process remain controversial. Here, we show MSCs isolated from mice lacking S-nitrosoglutathione reductase, denitrosylase protein S-nitrosylation, exhibited decreased increased osteoblastogenesis compared with WT MSCs. Consistent cellular phenotype,...