A5048294064- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Kidney Stones and Urolithiasis Treatments
- Parathyroid Disorders and Treatments
- Renal and related cancers
- Renal function and acid-base balance
- Pediatric Urology and Nephrology Studies
- MicroRNA in disease regulation
- Bone health and treatments
- Bone health and osteoporosis research
- Cancer-related molecular mechanisms research
- Magnesium in Health and Disease
- Cancer, Hypoxia, and Metabolism
- Chronic Kidney Disease and Diabetes
- Electrolyte and hormonal disorders
- Sepsis Diagnosis and Treatment
- Bone and Dental Protein Studies
- Pluripotent Stem Cells Research
- Acute Kidney Injury Research
- Therapeutic Uses of Natural Elements
- Marine and environmental studies
- Renal and Vascular Pathologies
- Electrospun Nanofibers in Biomedical Applications
- Dialysis and Renal Disease Management
- Bone Metabolism and Diseases
Universidade de São Paulo
2015-2025
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2020-2025
University of Miami
2011-2019
Hospital Israelita Albert Einstein
2016
Universidade Federal de São Paulo
2004-2014
Miami Transplant Institute
2014
Stem Cell Institute
2013
Although human mesenchymal stem cells (hMSCs) have been tested in ischemic cardiomyopathy, few studies exist chronic nonischemic dilated cardiomyopathy (NIDCM).The authors conducted a randomized comparison of safety and efficacy autologous (auto) versus allogeneic (allo) bone marrow-derived hMSCs NIDCM.Thirty-seven patients were to either allo- or auto-hMSCs 1:1 ratio. Patients recruited between December 2011 July 2015 at the University Miami Hospital. received (100 million) by...
This prospective study aimed to identify risk factors associated with progression stage 3 chronic kidney disease (CKD) and the occurrence of stone formation or growth following nephrectomy for urolithiasis. From January 2006 May 2013, patients undergoing urolithiasis were enrolled. Renal function was assessed using estimated glomerular filtration rate (eGFR) via Chronic Kidney Disease Epidemiology Collaboration equation, while events detected computed tomography. Among 107 followed an...
Although nitric oxide (NO) signaling promotes differentiation and maturation of endothelial progenitor cells, its role in the mesenchymal stem cells (MSCs) into remains controversial. We tested NO MSCs derived from WT mice homozygous for a deletion S -nitrosoglutathione reductase (GSNOR −/− ), denitrosylase that regulates -nitrosylation. GSNOR exhibited markedly diminished capacity vasculogenesis an vitro Matrigel tube–forming assay vivo relative to MSCs. This decrease was associated with...
Bone marrow-derived mesenchymal stem cells (MSCs) are a common precursor of both adipocytes and osteoblasts. While it is appreciated that PPARγ regulates the balance between adipogenesis osteogenesis, roles additional regulators this process remain controversial. Here, we show MSCs isolated from mice lacking S-nitrosoglutathione reductase, denitrosylase protein S-nitrosylation, exhibited decreased increased osteoblastogenesis compared with WT MSCs. Consistent cellular phenotype,...
Activation of an osteogenic transcriptional program contributes to the initiation aortic calcification in atherosclerosis.The role microRNAs regulating is understudied.We tested hypothesis that miR-30e regulates bone marrow-derived mesenchymal stem cells (MSCs), smooth muscle (SMCs), and ApoE 2/2 mice. Methods resultsIn aortas wild-type mice, we found highly expressed medial SMCs.In old transcripts are down-regulated inverse relation markers Runx2, Opn, Igf2.In vitro, over-expression reduced...
The presence of tissue specific precursor cells is an emerging concept in organ formation and homeostasis. Several progenitors are described the kidneys. However, their identity as a true stem cell remains elusive. Here, we identify neonatal kidney-derived c-kit(+) population that fulfills all criteria cell. These were found thick ascending limb Henle's loop exhibited clonogenicity, self-renewal, multipotentiality with differentiation capacity into mesoderm ectoderm progeny. Additionally,...
Resumo A prevalência da nefrolitíase tem aumentado nos últimos anos, afetando entre 10% e 15% população. doença litiásica está associada a comorbidades sistêmicas, como cardiovascular, diabetes mellitus obesidade. primeira Diretriz de Litíase Urinária Sociedade Brasileira Nefrologia foi publicada em 2002, desde então o acúmulo novos estudos clínicos diretrizes justificou revisão do tema. Este documento, elaborado pelo Comitê Nefrolitíase Nefrologia, reflete os avanços no manejo dos pacientes...
Abstract The prevalence of nephrolithiasis has been increasing in recent years, affecting approximately 10% and 15% the population. Kidney stone disease is associated with systemic comorbidities such as cardiovascular disease, diabetes mellitus, obesity. first Nephrolithiasis Guideline by Brazilian Society Nephrology was published 2002, since then, accumulation new clinical studies guidelines justified a review subject. This updated document, prepared Committee Nephrology, reflects advances...
Peritoneal fibrosis (PF) represents a long-term complication of peritoneal dialysis (PD), affecting membrane (PM) integrity and function. Understanding the mechanisms underlying PF development in an uremic environment aiming alternative therapeutic strategies for treating this process is great interest. The aim study was to analyze effects tamoxifen (TAM) recombinant BMP7 (rBMP7) experimental model developed rats.To mimic clinical situation patients on PD, combo model, characterized by...
The estrogen receptor (ER) gene has been considered as a candidate genetic marker for osteoporosis, and PvuII XbaI polymorphisms of the ERα have associated with low bone mineral density (BMD). We investigated whether ER polymorphism could predict response BMD in 28 postmenopausal women on hemodialysis marked osteopenia or randomized to receive raloxifene, selective modulator (SERM), placebo 1 year. was assessed by dual X-ray absorptiometry restriction fragment-length determined using...
This study aimed to determine the expression of osteoprotegerin, receptor activator nuclear factor kappaB ligand, interleukin-1alpha, transforming growth factor-beta, and basic fibroblast in stone-forming patients with idiopathic hypercalciuria.Immunohistochemical analysis was performed undecalcified bone samples previously obtained from 36 transiliac biopsies who had hypercalciuria whose histomorphometry shown lower volume, increased resorption, prolonged mineralization lag time.Bone ligand...
Sepsis induces organ dysfunction due to overexpression of the inflammatory host response, resulting in cardiopulmonary and autonomic dysfunction, thus increasing associated morbidity mortality. Wharton's jelly-derived mesenchymal stem cells (WJ-MSCs) express genes secrete factors with anti-inflammatory properties, neurological immunological protection, as well improve survival experimental sepsis. The cholinergic pathway (CAP) is mediated by α7-nicotinic acetylcholine receptors (α7nAChRs),...
Background Immunosuppressive regimen is associated with several metabolic adverse effects. Bone loss and fractures are frequent after transplantation involve multifactorial mechanisms. Methods A retrospective analysis of 130 patients submitted to simultaneous pancreas-kidney (SPKT) an identification risk factors involved in de novo Charcot neuroarthropathy by multivariate were used; P<0.05 was considered significant. Results diagnosed 4.6% SPKT recipients during the first year. Cumulative...
Increased bone resorption, low formation, and abnormal mineralization have been described in stone formers with idiopathic hypercalciuria. It has previously shown that the receptor activator of NF-κB ligand mediates resorption hypercalciuria (IH). The present study aimed to determine expression fibroblast growth factor 23 (FGF-23), vitamin D (VDR), sclerostin tissue from IH formers.Immunohistochemical analysis was performed undecalcified samples obtained for histomorphometry 30 transiliac...
Kidney-derived c-kit+ cells exhibit progenitor/stem cell properties and can regenerate epithelial tubular following ischemia-reperfusion injury in rats. We therefore investigated whether contribute to podocyte repair a rat model of acute proteinuria induced by puromycin aminonucleoside (PAN), the experimental prototype human minimal change disease early stages focal segmental glomerulosclerosis. found that accelerated kidney recovery improving foot process effacement (foot width was lower...
Kidney-derived c-Kit+ cells exhibit progenitor/stem cell properties in vitro (self-renewal capacity, clonogenicity, and multipotentiality). These can regenerate epithelial tubular following ischemia-reperfusion injury accelerate foot processes effacement reversal a model of acute proteinuria rats. Several mechanisms are involved kidney regeneration by kidney-derived cells, including engraftment differentiation into renal-like structures, such as tubules, vessels, podocytes. Moreover,...
The mechanisms underlying mesenchymal stem cells' (MSC) suppressive potency are largely unknown. We here show that highly human adipose tissue-derived MSC (AdMSC) display and induce a differential immunologic profile, upon ongoing AdMSC activity, promoting: (i) early correlated inhibition of IFN-γ TNF-α production, along IL-10 increase, (ii) CD73+Foxp3+Treg subset expansion, (iii) specific correlations between gene expression increases, such as: MMP9 with CCL22, TNF, FASL, RUNX3, SEMAD4 in...
Sepsis is a dysregulated hyperinflammatory disease caused by infection. leads to multiple organ dysfunction syndrome (MODS), which associated with high rates of mortality. The cecal ligation and puncture (CLP) model has been widely used in animals become the gold-standard method replicating features sepsis humans. Despite several studies modified CLP protocols, there are still open questions regarding multifactorial determinants its reproducibility medical significance. In our protocol, also...