A5103159490- Plant Disease Resistance and Genetics
- Wheat and Barley Genetics and Pathology
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- RNA Interference and Gene Delivery
- Genetic Mapping and Diversity in Plants and Animals
- Plant-Microbe Interactions and Immunity
- Plant Gene Expression Analysis
- Plant Pathogens and Resistance
- Plant Molecular Biology Research
- Legume Nitrogen Fixing Symbiosis
- Neurotransmitter Receptor Influence on Behavior
- Plasma Applications and Diagnostics
- Food composition and properties
- Phytase and its Applications
- Photosynthetic Processes and Mechanisms
- Rice Cultivation and Yield Improvement
- RNA regulation and disease
- Catalytic Processes in Materials Science
- Plant Stress Responses and Tolerance
- Viral Infections and Immunology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Cytomegalovirus and herpesvirus research
California Institute of Technology
2020-2025
Northwest A&F University
2015-2025
City University of Hong Kong
2024
Huaiyin Institute of Technology
2010-2024
Children's Hospital of Chongqing Medical University
2013-2023
Chongqing Medical University
2013-2023
Research Network (United States)
2023
Pasadena City College
2023
National Clinical Research
2022
China International Science and Technology Cooperation
2022
Summary Grain size is a dominant component of grain weight in cereals. Earlier studies have shown that OsGS5 plays major role regulating both and rice via promotion cell division. In this study, we isolated TaGS5 homoeologues wheat mapped them on chromosomes 3A, 3B 3D. Temporal spatial expression analysis showed were preferentially expressed young spikes developing grains. Two alleles TaGS5‐3A , TaGS5‐3A‐T TaGS5‐3A‐G identified accessions, functional marker was developed to discriminate...
A brain circuit to control alcohol intake Most people are exposed at some point in their lives, but only a small fraction will develop compulsive drinking disorder. Siciliano et al. first established behavioral measure assess how predisposition interacts with experience produce subset of mice (see the Perspective by Nixon and Mangieri). In search underlying neurobiological mechanism, they discovered that discrete between medial prefrontal cortex brainstem is central for development drinking....
Gene therapy offers great promise in addressing neuropathologies associated with the central and peripheral nervous systems (CNS PNS). However, genetic access remains difficult, reflecting critical need for development of effective non-invasive gene delivery vectors across species. To that end, we evolved adeno-associated virus serotype 9 (AAV9) capsid mice validated two capsids, AAV-MaCPNS1 AAV-MaCPNS2, rodent species (mice rats) non-human primate (NHP) (marmosets rhesus macaques)....
Abstract Crossing the blood–brain barrier in primates is a major obstacle for gene delivery to brain. Adeno-associated viruses (AAVs) promise robust, non-invasive from bloodstream However, unlike rodents, few neurotropic AAVs efficiently cross non-human primates. Here we report on AAV.CAP-Mac, an engineered variant identified by screening adult marmosets and newborn macaques, which has improved efficiency brains of multiple primate species: marmoset, rhesus macaque green monkey. CAP-Mac...
Abstract Delivering genes to and across the brain vasculature efficiently specifically species remains a critical challenge for addressing neurological diseases. We have evolved adeno-associated virus (AAV9) capsids into vectors that transduce endothelial cells following systemic administration in wild-type mice with diverse genetic backgrounds, rats. These AAVs also exhibit superior transduction of CNS non-human primates (marmosets rhesus macaques), ex vivo human slices, although tropism is...
Abstract Cell-type-specific regulatory elements such as enhancers can direct expression of recombinant adeno-associated viruses (AAVs) to specific cell types, but this approach is limited by the relatively small packaging capacity AAVs. In study, we used spatial genomics show that transcriptional crosstalk between individual AAV genomes provides a general method for cell-type-specific large cargo separating distally acting into second genome. We identified and profiled in carrying 11...
A barrier to advancing engineered adeno-associated viral vectors (AAVs) for precision access cell subtypes is a lack of high-throughput, high-resolution assays characterize in vivo transduction profiles. In this study, we developed an ultrasensitive, sequential fluorescence situ hybridization (USeqFISH) method spatial transcriptomic profiling endogenous and RNA with short barcode intact tissue volumes by integrating hydrogel-based clearing, enhanced signal amplification multiplexing using...
The blood-brain barrier (BBB) presents a major challenge for delivering large molecules to study and treat the central nervous system. This is due in part scarcity of targets known mediate BBB crossing. To identify novel targets, we leverage panel adeno-associated viruses (AAVs) previously identified through mechanism-agnostic directed evolution improved transcytosis. Screening potential cognate receptors enhanced crossing, two targets: murine-restricted LY6C1 widely conserved carbonic...
The glossy varieties (A14 and Jing 2001) glaucous (Fanmai 5 Shanken 99) of wheat (Triticum aestivum L.) were selected for evaluation developmental changes in the composition morphology cuticular waxes on leaves spikes. results provide us with two different wax development patterns between leaf spike. general accumulation trend total load spike surfaces is first to increase then decrease during growth period, but these caused by compound classes Developmental mainly result variations alcohols...
Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is a devastating foliar disease that affects common wheat and barley throughout the world. The reasonable deployment of adult plant resistance (APR) varieties one best methods for controlling this disease. Wheat landraces are valuable resources identifying genes/QTLs responsible resistance. Humai 15 Chinese spring landrace it has exhibited adequate levels APR to prevalent Pst races in field environments many years. In study,...
Auxin/indoleacetic acid (Aux/IAA) proteins play an important regulatory role in the developmental process of plants and their responses to stresses. A previous study has shown that constitutive expression OsIAA18, Aux/IAA transcription factor gene rice improved salt osmotic tolerance transgenic Arabidopsis plants. However, little work is known about functions OsIAA18 regulating abiotic stress rice. In this study, was introduced into cultivar, Zhonghua 11 overexpression exhibited...
Abstract Innovations in high-resolution optical imaging have allowed visualization of nanoscale biological structures and connections. However, super-resolution fluorescence techniques, including both optics-oriented sample-expansion based, are limited quantification throughput especially tissues from photobleaching or quenching the fluorophores, low-efficiency non-uniform delivery probes. Here, we report a general vibrational strategy, termed VISTA, for scalable label-free interrogations...
The ability to recognize motivationally salient events and adaptively respond them is critical for survival. Here, we tested whether dopamine (DA) neurons in the dorsal raphe nucleus (DRN) contribute this process both male female mice. Population recordings of DRN DA during associative learning tasks showed that their activity dynamically tracks motivational salience, developing excitation reward-paired shock-paired cues. response reward-predicting cues was diminished after satiety,...
Wheat sheath blight caused by the necrotic fungal pathogen Rhizoctonia cerealis is responsible for severe damage to bread wheat. Reactive oxygen species (ROS) are vital stress resistance plants and their homeostasis plays an important role in wheat blight. Valine-glutamine (VQ) proteins play roles plant growth development, responses biotic abiotic stresses. However, functional mechanism mediated VQ protein response via ROS regulation unclear. In this study, we identified TaVQ22 containing...
Deep-learning-based methods for protein structure prediction have achieved unprecedented accuracy, yet their utility in the engineering of protein-based binders remains constrained due to a gap between ability predict structures candidate proteins and toprioritize by potential bind target. To bridge this gap, we introduce Automated Pairwise Peptide-Receptor Analysis Screening Engineered (APPRAISE), method predicting target-binding propensity engineered proteins. After generating structural...
Plant microRNAs (miRNAs) have been shown to play critical roles in plant development. In this study, we employed small RNA combined with degradome sequencing survey development-related miRNAs and their validated targets during wheat grain A total of 186 known 37 novel were identified four libraries. Moreover, a miRNA-like long hairpin locus was first produce 21~22-nt phased siRNAs that act trans cleave target mRNAs. comparison the miRNAomes revealed 55 miRNA families differentially expressed...