A5077883031- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Viral Infections and Immunology Research
- Nuclear Structure and Function
- Animal Genetics and Reproduction
- Genomics and Chromatin Dynamics
- Cancer-related gene regulation
- Wnt/β-catenin signaling in development and cancer
- Genetics and Neurodevelopmental Disorders
Board of the Swiss Federal Institutes of Technology
2021-2025
ETH Zurich
2021-2025
DKFZ-ZMBH Alliance
2021-2024
German Cancer Research Center
2021-2024
Heidelberg University
2021-2024
Biomolecular condensation has emerged as an important mechanism to control various cellular processes through the formation of membraneless organelles. Fluorescent protein tags have been extensively used study and properties condensates in vitro vivo, but there is evidence that may perturb proteins. In this study, we carefully assess effects on yeast DEAD-box ATPase Dhh1, a central regulator processing bodies (P-bodies), which are biomolecular involved mRNA metabolism. We show fluorescent...
The role of WNT/β-catenin signalling in mouse neocortex development remains ambiguous. Most studies demonstrate that regulates progenitor self-renewal but others suggest it can also promote differentiation. Here we explore the WNT/STOP signalling, which stabilizes proteins during G2/M by inhibiting glycogen synthase kinase (GSK3)-mediated protein degradation. We show mice mutant for cyclin Y and Y-like 1 (Ccny/l1), key regulators display reduced neurogenesis developing neocortex....
A hallmark of Huntington's disease (HD) is a prolonged polyglutamine sequence in the huntingtin protein and, correspondingly, an expanded cytosine, adenine, and guanine (CAG) triplet repeat region mRNA. majority studies investigating pathology were concerned with toxic protein, but mRNA moved into focus due to its recruitment RNA foci emerging novel therapeutic approaches targeting CAG-RNA that it forms stable hairpin vitro which seems be crucial for specific interactions. Using in-cell...
DDX RNA helicases promote processing, but DDX3X also activates casein kinase 1 (CK1ε). We show that other proteins stimulate the protein activity of CK1ε and this extends to 2 (CK2). CK2 enzymatic was stimulated by various at high substrate concentrations. DDX1, DDX24, DDX41, DDX54 were required for full in vitro Xenopus embryos. Mutational analysis indicated CK1 stimulation engages its binding not catalytic motifs. Mathematical modeling enzyme kinetics stopped-flow spectroscopy showed...
Biomolecular condensation has emerged as an important mechanism to control various cellular processes through the formation of membraneless organelles. Fluorescent protein tags have been extensively used study and properties condensates in vitro vivo, but there is evidence that may perturb proteins. In this study, we carefully assess effects on yeast DEAD-box ATPase Dhh1, a central regulator processing bodies (P-bodies), which are biomolecular involved mRNA metabolism. We show fluorescent...
DEAD box (DDX) RNA helicases are a large family of ATPases, many which have unknown functions. There is emerging evidence that besides their role in biology, DDX proteins may stimulate protein kinases. To investigate if kinase-DDX interaction more widespread phenomenon, we conducted three orthogonal large-scale screens, including proteomics analysis with 32 helicases, array profiling, and kinome-wide vitro kinase assays. We retrieved Ser/Thr kinases as prominent interactors report hundreds...
Abstract DDX RNA helicases promote processing but DDX3X is also known to activate casein kinase 1 ε (CK1ε). Here we show that not only protein stimulation a latent property of other proteins towards CK1ε, this extends 2 (CK2α2) as well. CK2α2 enzymatic activity stimulated by variety and identify DDX1/24/41/54 physiological activators required for full in vitro Xenopus embryos. Mutational analysis reveals CK1 CK2 engages its binding-but catalytic motifs. Mathematical modelling enzyme kinetics...