A5062178362- Extracellular vesicles in disease
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Advanced Biosensing Techniques and Applications
- RNA Interference and Gene Delivery
- Conducting polymers and applications
- SARS-CoV-2 detection and testing
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Nanoplatforms for cancer theranostics
- Perovskite Materials and Applications
- MicroRNA in disease regulation
- Bone Tissue Engineering Materials
- Nanopore and Nanochannel Transport Studies
- Nanofabrication and Lithography Techniques
- Monoclonal and Polyclonal Antibodies Research
- 3D Printing in Biomedical Research
- Organic Electronics and Photovoltaics
- Immunotherapy and Immune Responses
- Quantum Dots Synthesis And Properties
- Viral gastroenteritis research and epidemiology
- Advanced Fiber Optic Sensors
- Cancer Cells and Metastasis
- Medical Imaging and Pathology Studies
- Bone health and treatments
Pennsylvania State University
2014-2022
Carnegie Mellon University
2020-2022
Jilin University
2019-2021
State Key Laboratory on Integrated Optoelectronics
2019-2021
Millennium Engineering and Integration (United States)
2014-2017
Tokyo Institute of Technology
2017
Tsinghua University
2013-2016
Park University
2015
Abstract Extracellular nanovesicles (ENV) released by many cells contain lipids, proteins, and nucleic acids that contribute to intercellular communication. ENVs have emerged as biomarkers therapeutic targets but they also been explored drug delivery vehicles. However, for the latter application, clinical translation has limited low yield inadequate targeting effects. ENV vectors with desired properties can be produced from parental engineered express membrane-bound ligands, or generated...
Abstract Bone metastasis occurs at ≈70% frequency in metastatic breast cancer. The mechanisms used by tumors to hijack the skeleton, promote bone metastases, and confer therapeutic resistance are poorly understood. This has led development of various models investigate interactions between cancer cells host marrow related physiological changes. However, it is challenging perform studies due difficulty periodic sampling. Herein, a bone‐on‐a‐chip (BC) reported for spontaneous growth 3D,...
Viruses pose serious infectious disease threats to humans and animals. To significantly decrease the mortality morbidity caused by virus infections, there is an urgent need of sensitive rapid point-of-care platforms for detection, especially in low-resource settings. Herein, we developed a smartphone-based platform highly selective detection avian influenza based on nanomaterial-enabled colorimetric detection. The 3D nanostructures, which serve as scaffold antibody conjugation capture virus,...
Rapid and simultaneous detection of multiple potential pathogens by portable devices can facilitate early diagnosis infectious diseases, allow for rapid effective implementation disease prevention treatment measures. The development a ZnO nanorod integrated microdevice as multiplex immunofluorescence platform highly sensitive selective avian influenza virus (AIV) is described. 3D morphology unique optical property the nanorods boost limit H5N2 AIV to low 3.6 × 103 EID50 mL-1 (EID50 : 50%...
Preparing high-quality perovskite film with large grain size and fewer trap states is of vital importance in boosting the efficiency stability solar cells (PSCs). However, it still difficult to obtain perfect MAPbI3 films by antisolvent treatment so far because small size, pinholes, numerous defects layers. Herein, acetonitrile (ACN) was introduced into chlorobenzene (CB) modify active layer. The results show that ACN could control ratio DMSO MAI-PbI2-DMSO intermediate phase effectively thus...
Immune checkpoint inhibitors (ICI) targeting PD-1/PD-L1 have been approved for the treatment of a variety cancers. However, efficacy antibody-based ICIs could be further improved by mitigating anti-drug antibodies, proteolytic cleavage, and on-target off-tumor toxicity. One strategy accomplishing this is through use extracellular vesicles (EVs), cell derived submicron with many unique properties. We constructed an engineered MDA-MB-231 line harvesting EVs. This was accomplished...
Endo-/lysosome escape is a major challenge in nanoparticle-based protein delivery for cancer therapy. To enhance the endo-/lysosomal and increase efficacy of delivery, current strategies mainly focus on destroying endo-/lysosomes by employing modified nanoparticles, such as pH-sensitive polyplexes, cell-penetrating peptides, photosensitive molecules. Herein, we hypothesize that pretreatment with empty nanocarriers might make occupied affect endo/lysosomal subsequently nanocarriers. We first...
Viral diseases are perpetual threats to human and animal health. Detection characterization of viral pathogens require accurate, sensitive, rapid diagnostic assays. For field clinical samples, the sample preparation procedures limit ultimate performance utility overall virus protocols. This study presents development a microfluidic device embedded with porous silicon nanowire (pSiNW) forest for label‐free size‐based point‐of‐care capture in continuous curved flow design. The pSiNW forests...
Nanoscale extracellular vesicles (nEVs) have recently demonstrated potential value in cancer diagnostics and treatment monitoring, but translation has been limited by technical challenges nEV isolation.
Triple negative breast cancer (TNBC) is a heterogeneous subset of characterized by its lack estrogen receptor, progesterone and human epidermal growth factor receptor 2 (HER2), which altogether prevents TNBC from being treated effectively. For many years, the treatment paradigms overall survival patients with have remained largely stagnant. Recent attempts to convert cold tumors hot promoting antigen presentation shown increased T cell infiltration significantly induced immune responses for...
The mechanism of cells passing through microconstrictions, such as capillaries and endothelial junctions, influences metastasis circulating tumor (CTCs) in vivo, well size-based enrichment CTCs vitro. However, very few studies observe translocation microconstrictions real time, thus the inherent biophysical is poorly understood. In this study, a multiplexed microfluidic device fabricated for real-time tracking cell under physiological pressure recording deformation whole nucleus,...
Quantum dot (QD) multicolor analysis was achieved by labeling the epidermal growth factor and antibody against receptor (anti-EGFR) using different QDs. On basis of fluorescence intensity two types QDs, amount factors (EGFs) in cells anti-EGFRs bound to EGFRs were analyzed. The functions anti-EGFR preventing HeLa from engulfing EGF inhibiting overproliferation also studied. Meanwhile, parallel conducted analyze heterogeneity at single-cell level a array. This provides novel approach for...
There has been a growing interest in the development of efficient flexible organic solar cells (OSCs) due to their unique capacity provide energy sources for electronics. To this end, it is required design compatible interlayer with low processing temperature and high electronic quality. In work, we present that quality ZnO fabricated from low-temperature (130 °C) sol–gel method can be significantly improved by doping an small molecule, TPT-S. The doped TPT-S, on one hand, passivates...
Abstract Conventional light sheet fluorescence microscopy (LSFM) utilizes two perpendicularly arranged objective lenses for optical excitation and detection, respectively. Such a configuration often limits the use of high‐numerical‐aperture (NA) objectives or requires specially designed long‐working‐distance objectives. Here, LSFM based on micro‐mirror array (MMA) to enable imaging with single lens is reported. The planar fluorescent emission excited by illumination collected same objective,...
The results of quantum dot (QD) probe preparation for multiplexed single-cell array staining and analysis are reported. By controlling the reaction temperature, time, ratio Cd to Se, multicolor CdSe QDs emitting fluorescence ranging from purple red in a safer, simpler, more convenient way than traditional methods is obtained. To detect cells using these oil-soluble QDs, they first coated with water-soluble thioglycolic aid (TGA) so that biocompatible multiwavelength bioprobes can be QDs'...
Achieving a highly crystalized perovskite active layer with dense, flat surface and large grain size is very significant for constructing efficient solar cells (PSCs). Regarding this, the solvent engineering plays an important role in optimizing crystallinity final device performance. 2-Methoxyethanol (2-ME) recently reported to be good processing of high-quality film. However, conventional one-step fabrication method, little attempt made introduce 2-ME as additive into...
A microfluidic device of porous silicon nanowire (PSNW) channel walls was fabricated as a point-of-care platform to capture nanoparticles (NPs) like viruses based on their sizes. PSNWs were synthesized the bottom and sidewalls micro channels with metal-assisted etching. The space between controlled NPs specific size. To enhance rate, meandering designed increase interaction by Dean flow. With this system, we demonstrated label-free N5H2 avian influenza virus capture. In addition, can be...