The use of a new indicator culture medium, Belo Horizonte is proposed for better colony recognition and presumptive identification Campylobacter pylori. This containing brain heart infusion sheep blood agar, was supplemented with 40 mg 2,3,5-triphenyltetrazolium chloride per liter in addition to vancomycin, nalidixic acid, amphotericin B. On pylori present unique golden colonies.

A new Helicobacter species that colonizes the colonic mucosa of Wistar and Holtzman rats was isolated characterized. This bacterium gram negative, its cells were rod shaped with pointed ends, protoplasmic cylinder entwined periplasmic fibers. It catalase oxidase positive, rapidly hydrolyzed urea, susceptible to metronidazole resistant cephalothin nalidixic acid. The organism microaerophilic grew at 42°C, a feature differentiates it from two other murine intestine colonizers, hepaticus...

Summary The ultrastructural features of a helical-shaped bacterium occurring inthe stomach pigs, within the mucus on mucosal surface antral pits,were examined. bacterial cell had three to eight spiral turns, flattened and truncated ends was approximately 4.0 μm long 0.6 wide.In some sections, up six flagella, about 22 nm in diameter, were seen arising from each pole. cytoplasm contained sparse, irregular granules,numerous ribosomes large single-layered membrane-bound granules. In flagella...

Objective To describe and compare the plantar pressures, temporal foot roll‐over, ground reaction forces (GRFs) between both limbs of subjects with unilateral transfemoral amputation those able‐bodied participants during walking. We also verify relevance a force plate pressure to discriminate changes in gait parameters limb loss. Design Cross‐sectional study. Setting Biomechanics laboratory. Subjects A total 14 21 participants. Methods used assess biomechanical while were walking at their...

Summary Tightly spiralled bacteria ("Gastrospirillum suis") were seen in the pyloric mucosa of stomach 13 (10.8%) 120 pigs that appeared clinically healthy at slaughter and fundic three (5.0%) out 60 pigs. The spiral organism could not be cultured from any pig. Chronic gastritis was observed 53 (44.2%) 7 (11.7%) with region comprised one animal (7.7%) normal mucosa, two (15.4%) "borderline gastritis", 10 (76.9%) chronic gastritis–in instance accompanied by signs activity (numerous...

This paper reports a suitable piezoresistive carbon nanotube (CNT)/PVDF composite capable of measuring the interface pressure within prosthetic stump/sockets. The transducers were prepared by hot pressing with CNTs sample concentrations up to 10 wt.%. sensibility (Gauge factor) composites was characterized using mechanical testing equipment and values reached 6.2. After optimization materials, tests conducted prototype placed in lower limb amputee patients, performing several standing...

We evaluated the accuracy of a 2nd generation ELISA to detect Helicobacter pylori infection in adults from developing country view variations sensitivity and specificity reported for different populations. studied 97 non-consecutive patients who underwent endoscopy evaluation dispeptic symptoms. The presence H. was determined antral biopsy specimens by culture, preformed urease test carbolfuchsin-stained smears. Patients were considered be positive if at least two three tests presented...

To purify and partially characterize a bacteriocin produced by Fusobacterium nucleatum strain.Following protein precipitation the effect of different treatments on F. strain named P12.2 isolated from patient with periodontitis was evaluated. The antagonistic activity intracellular fraction obtained at 80% ammonium sulphate preserved pH values 6.0 to 9.0 showed be sensitive high temperatures treatment proteases. submitted sequential steps gel filtration, ion exchange, reverse phase...

Individuals with transfemoral amputation must adapt their gait to the absence of one natural limb, causing an impact throughout body. A better understanding these changes would provide safer and more efficient development prevention rehabilitation strategies. The aim this study was compare center pressure (CoP) variables between amputated limb sound subjects able-bodied during gait. FootScan plate (RsScan, Olen, Belgium) used record CoP motion 14 individuals (56.7 ± 11.7 years old) 21...

An enzyme isotopic assay was used to determine the histamine concentration in gastric mucosa of patients positive for Helicobacter pylori with (n = 11) and without duodenal ulceration 9) negative controls 7). A significant difference observed when content H subjects compared that patients. On other hand, there no between those ulceration. had significantly lower concentrations than subjects. The might be due increased release which could turn induce acid secretion.

The purpose of this study was to investigate the effect oxidative stress on physiological and genetic characteristics Fusobacterium nucleatum its interference microbial identification methods.Fus. ssp. ATCC 25586 (wt-strain) an oxidative-stress-adapted strain derived from wt-strain (aero-strain) were employed in study. Cell-free crude protein extracts obtained both strains differentially expressed proteins identified by two-dimensional electrophoresis. Bacterium performed conventional...

Helicobacter trogontum is a micro-aerophilic urease-positive bacterium that has recently been isolated from the intestinal mucosa of rats. The purpose this investigation was to study ultrastructural details micro-organism in both pure culture and gastrointestinal tract germ-free mice infected with H. trogontum. fusiform slightly spiral gram-negative cell, 4–6 μm long 0.6–0.7 wide, four seven bipolar sheathed flagella. cytoplasm presented several irregular also globular granules. On each side...

Summary To establish an experimental model to study gastric spiral non-cultivable bacteria, 30 4-week-old female CFW (LOB) mice were inoculated with porcine mucus containing “Gastrospirillum suis” and 25 without “G. suis”. Mice examined 3, 7, 14, 21, 28 60 days after inoculation. Fragments from the membranous, oxyntic antral mucosa duodenal obtained for histological microbiological analysis. Tightly spiralled bacteria seen in smears sections of all G. suis-infected mice. The pre-formed...

Patients with the digestive form of Chagas'disease frequently present chronic gastritis. As microorganism Helicobacter pylori is now accepted as most common cause human gastritis, work was undertaken to verify a possible relationship between presence this bacterium and inflammatory changes antral mucosa in chagasic patients. Seventeen chagasics, megaesophagus or megacolon were studied. Fragments from two different regions obtained by endoscopy, fixed 4% neutral formaldehyde embedded...

El presente artículo en honor a Patrick Charaudeau tiene dos objetivos centrales. En un primer momento, haremos una narrativa de cómo la teoría semiolingüística se estableció y desarrolló como campo investigación Facultad Letras/UFMG-Brasil. segundo demostraremos las contribuciones investigadores brasileños teniendo punto central reflexiones adaptaciones del cuadro los sujetos lenguaje su aplicabilidad diferentes tipologías discursivas. Para concluir, tomando por base anteriores, sobre...

In the present study we compared inoculation of swine gastric mucus into stomach mice, urease test and carbolfuchsin-stained smears for diagnosis infection with "Gastrospirillum suis" ("Helicobacter heilmannii" type 1), an uncultivated tightly spiralled bacterium. Fragments obtained from antral oxyntic mucosa 50 slaughtered pigs were used test, obtaining scrapings mouse inoculation. The mice killed by spinal dislocation 10 days after fragments spiral bacterium identification (urease smears)....