A5000835898- CRISPR and Genetic Engineering
- RNA Research and Splicing
- RNA regulation and disease
- RNA Interference and Gene Delivery
- Genetics, Aging, and Longevity in Model Organisms
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Nuclear Structure and Function
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Cytomegalovirus and herpesvirus research
- Advanced biosensing and bioanalysis techniques
University of Maryland, College Park
2017-2024
NYU Langone Health
2023
New York University
2021-2023
LINE-1 (L1) is the only autonomously active retrotransposon in human genome, and accounts for 17% of genome. The L1 mRNA encodes two proteins, ORF1p ORF2p, both essential retrotransposition. ORF2p has reverse transcriptase endonuclease activities, while a homotrimeric RNA-binding protein with poorly understood function. Here, we show that condensation critical Using combination biochemical reconstitution live-cell imaging, demonstrate electrostatic interactions trimer conformational dynamics...
Stable epigenetic changes appear uncommon, suggesting that typically dissipate or are repaired. Changes stably alter gene expression across generations presumably require particular conditions currently unknown. Here we report a minimal combination of cis-regulatory sequences can support permanent RNA silencing single-copy transgene and its derivatives in C. elegans simply upon mating. Mating disrupts competing RNA-based mechanisms to initiate last for >300 generations. This stable requires...
Since double-stranded RNA (dsRNA) is effective for silencing a wide variety of genes, all genes are typically considered equivalent targets such interference (RNAi). Yet, loss some regulators RNAi in the nematode Caenorhabditis elegans can selectively impair genes. Here, we show that selective requirements be explained by an intersecting network acting on with differences their metabolism. In this network, Maelstrom domain-containing protein RDE-10, intrinsically disordered MUT-16, and...
Long interspersed nuclear element–1 (LINE-1) is an autonomous retrotransposon that makes up a substantial portion of the human genome, contributing to genetic diversity and genome evolution. LINE-1 encodes two proteins, ORF1p ORF2p, both essential for successful retrotransposition. ORF2p has endonuclease reverse transcription activity, while binds RNA. Many copies assemble onto RNA form ribonucleoprotein (RNP) condensate. However, function these condensates in life cycle remains unclear....
Abstract The cell interior is highly crowded and far from thermodynamic equilibrium. This environment can dramatically impact molecular motion assembly, therefore influence subcellular organization biochemical reaction rates. These effects depend strongly on length-scale, with the least information available at important mesoscale (10-100 nanometers), which corresponds to size of crucial regulatory molecules such as RNA polymerase II. It has been challenging study physical properties...
Abstract Heritable gene silencing has been proposed to rely on DNA methylation, histone modifications, and/or non-coding RNAs in different organisms. Here we demonstrate that multiple RNA-mediated mechanisms with distinct and easily detectable molecular signatures can underlie heritable of the same open-reading frame nematode C. elegans . Using two-gene operons, reveal three cases gene-selective provide support for transmission epigenetic changes through RNA independent chromatin would...
Since double-stranded RNA (dsRNA) is effective for silencing a wide variety of genes, all genes are typically considered equivalent targets such interference (RNAi). Yet, loss some regulators RNAi in the nematode C. elegans can selectively impair genes. Here we show that selective requirements be explained by an intersecting network acting on with differences their metabolism. In this network, Maelstrom domain-containing protein RDE-10, intrinsically disordered MUT-16, and Argonaute NRDE-3...
Since double-stranded RNA (dsRNA) is effective for silencing a wide variety of genes, all genes are typically considered equivalent targets such interference (RNAi). Yet, loss some regulators RNAi in the nematode C. elegans can selectively impair genes. Here we show that selective requirements be explained by an intersecting network acting on with differences their metabolism. In this network, Maelstrom domain-containing protein RDE-10, intrinsically disordered MUT-16, and Argonaute NRDE-3...
Since double-stranded RNA (dsRNA) is effective for silencing a wide variety of genes, all genes are typically considered equivalent targets such interference (RNAi). Yet, loss some regulators RNAi in the nematode Caenorhabditis elegans can selectively impair genes. Here, we show that selective requirements be explained by an intersecting network acting on with differences their metabolism. In this network, Maelstrom domain-containing protein RDE-10, intrinsically disordered MUT-16, and...
Long Interspersed Nuclear Element-1 (LINE-1) is an autonomous retrotransposon that makes up a significant portion of the human genome, contributing to genetic diversity and genome evolution. LINE-1 encodes two proteins, ORF1p ORF2p, both essential for successful retrotransposition. ORF2p has endonuclease reverse transcription activity, while binds RNA. Many copies assemble onto RNA form ribonucleoprotein complex (RNP). However, functional role RNPs in life cycle unclear. Using reconstitution...
Since double-stranded RNA (dsRNA) is effective for silencing a wide variety of genes, all genes are typically considered equivalent targets such interference (RNAi). Yet, loss some regulators RNAi in the nematode C. elegans can selectively impair genes. Here we show that selective requirements be explained by an intersecting network acting on with differences their metabolism. In this network, Maelstrom domain-containing protein RDE-10, intrinsically disordered MUT-16, and Argonaute NRDE-3...
Abstract Changes in gene expression that last for multiple generations without changes sequence have been reported many plants and animals 1–3 . Cases of such transgenerational epigenetic inheritance (TEI) could support the ancestral origins some diseases drive evolutionary novelty. Here, we report stably expressed sequences C. elegans features provide a barrier against TEI. By using double-stranded RNA (dsRNA) targeting same different genes, show genes typically recover from silencing...