A5007884519- Extracellular vesicles in disease
- Chronic Kidney Disease and Diabetes
- MicroRNA in disease regulation
- Diabetes Treatment and Management
- Cancer-related molecular mechanisms research
- Dialysis and Renal Disease Management
- Genetic and Kidney Cyst Diseases
- Advanced Glycation End Products research
- Pancreatic function and diabetes
- Autophagy in Disease and Therapy
- Renal and related cancers
- Peptidase Inhibition and Analysis
- Parathyroid Disorders and Treatments
- Connective Tissue Growth Factor Research
- Cardiovascular Disease and Adiposity
- Immune cells in cancer
- Renal Diseases and Glomerulopathies
- Adipose Tissue and Metabolism
- Cancer Cells and Metastasis
- Genetic Syndromes and Imprinting
- Liver Disease Diagnosis and Treatment
- Metabolism, Diabetes, and Cancer
- Circular RNAs in diseases
- Hormonal Regulation and Hypertension
- Adipokines, Inflammation, and Metabolic Diseases
Southern Medical University
2016-2025
Nanfang Hospital
2016-2025
Hong Kong Polytechnic University
2022
Increasing evidence indicates that brown adipose tissue (BAT) transplantation enhances whole-body energy metabolism in a mouse model of diet-induced obesity. However, it remains unclear whether BAT also has such beneficial effects on genetically obese mice. To address this issue, we transplanted from C57/BL6 mice into the dorsal subcutaneous region age- and sex-matched leptin deficient Ob/Ob Interestingly, led to significant reduction body weight gain with increased oxygen consumption...
The dipeptidyl peptidase-4 inhibitor sitagliptin, a new anti-diabetic medicine, is effective in treating type 2 diabetes mellitus by increasing the activation and duration of action glucagon-like peptide-1. Since atherosclerosis main pathological feature diabetic cardiovascular complications, it important to investigate anti-atherosclerotic effect sitagliptin explore relevant mechanisms. Male apolipoprotein-E-knockout mice were randomly divided into two groups fed either high-fat diet (HFD)...
Background . miR-192, miR-194, and miR-215 are enriched in the kidney play roles pathogenesis of diabetic nephropathy (DN). Extracellular vesicles (EVs) can be detected body fluids may serve as disease biomarkers. Methods Eighty type 2 diabetes patients with normoalbuminuria (<mml:math xmlns:mml="http://www.w3.org/1998/Math/MathML" id="M1"><mml:mi>n</mml:mi><mml:mo>=</mml:mo><mml:mn fontstyle="italic">30</mml:mn></mml:math>), microalbuminuria...
Diabetic kidney disease is a renal microvascular caused by diabetes, known as one of the most serious and lethal complications diabetes. Early hypertrophy main pathological feature, which gradually leads to deposition glomerular extracellular matrix tubulointerstitial fibrosis, eventually developing irreversible structural damage kidneys. Autophagy cell self-homeostatic mechanism that activated under stress conditions may serve protective response survival fibrogenic cells. MicroRNA (miRNA)...
Backgroud: Diabetic nephropathy is one of the most frequent causes end-stage renal disease and associated with proliferation glomerular mesangial cells (MCs) excessive production extracellular matrix (ECM). Several studies have shown that early growth response factor 1 (Egr1) plays a key role in fibrosis by regulating expression genes encoding ECM components. However, whether Egr1 also contributes to diabetic unclear.In present study, we compared kidneys from OLETF rats spontaneous type 2...
Albumin absorbed by renal tubular epithelial cells induces inflammation and plays a key role in promoting diabetic kidney disease (DKD) progression. Macrophages are prominent inflammatory the kidney, their there is dependent on phenotypes. However, whether albuminuria influences macrophage phenotypes underlying mechanisms during development of DKD still unclear. We found that M1 macrophage-related markers were increased diabetes mellitus (DM) mouse tissues with DKD, coculture extracellular...
In diabetic patients complicated with colorectal cancer (CRC), metformin treatment was reported to have diverse correlation CRC-specific mortality. laboratory studies, affect the survival of stem cells (CSCs) in breast and pancreatic cancers glioblastoma. Although cscs play a critical role resistance 5-fluorouracil (5-FU) chemotherapy CRC patients, effect on synergistic combination 5-FU are not reported. present study pathological examinations were performed 86 type 2 DM who had been divided...
Abstract MicroRNAs (miRNAs) play vital roles in the development of diabetic nephropathy. Here, we compared protective efficacies miR-26a and miR-30c renal tubular epithelial cells (NRK-52E) determined whether they demonstrated additive effects attenuation fibrosis. TGFβ1 suppressed expression but up-regulated pro-fibrotic markers NRK-52E cells, these changes were also found kidney cortex 40-week-old Otsuka Long-Evans Tokushima fatty (OLETF) rats. Bioinformatic analyses luciferase assays...
Klotho, an antiaging protein, has been shown to play a protective role in renal tubular epithelial-mesenchymal transition (EMT) during the development of diabetic kidney disease (DKD). Long noncoding RNAs (lncRNAs) participate progression EMT many diseases. However, effect Klotho on lncRNAs DKD is still unknown. In this study, we found that overexpression high-fat diet (HFD)- and streptozotocin (STZ)-induced mice significantly inhibited expression lncRNA nuclear-enriched abundant transcript...
Abstract Background Albuminuria is a hallmark of diabetic kidney disease (DKD) that promotes its progression, leading to renal fibrosis. Renal macrophage function complex and influenced by metabolic status. However, the state macrophages impact albuminuria on are poorly understood. Methods Extracellular vesicles (EVs) from tubular epithelial cells (HK-2) were evaluated using transmission electron microscopy, nanoparticle tracking analysis western blotting. Glycolytic enzyme expression in...
Extracellular vesicles (EVs), which contain microRNA (miRNA), constitute a novel means of cell communication that may contribute to the inevitable expansion renal fibrosis during diabetic kidney disease (DKD). Exendin-4 is effective for treating DKD through its action on GLP1R. However, effect exendin-4 EV miRNA expression and development remains unknown. In this study, we found EVs derived from HK-2 cells pre-treated with high glucose (Ex-HG), were taken up by normal cells, resulted in...
Renal fibrosis is at the core of various renal diseases, including diabetic kidney disease (DKD). Long noncoding RNAs (lncRNAs) are known players in regulation fibrosis. However, their expression and function DKD still need to be elucidated. The purpose this study was assess how lncRNA GAS5 regulates its mechanism TGF-β1-treated proximal tubular cell.In study, upregulated both HK-2 cells kidneys HDF/STZ mice. Knockdown relieved epithelial This effect mediated by downregulation functional...
Background/Aims: Glucagon-like peptide-1 (GLP-1), which counteracts insulin resistance in humans with type 2 diabetes, has been shown to ameliorate diabetic nephropathy experimental models. However, the mechanisms through GLP-1 modulates renal function remained illdefined. The present study investigated putative underlying effects of exendin-4, a analog, on mesangial cell proliferation and fibronectin. Methods: Rat cells (MCs) were treated exendin-4 under high glucose conditions....
Accumulating evidence indicates that proteinuria promotes the progression of diabetic kidney disease (DKD) and induces renal epithelial tubular cell epithelial‐to‐mesenchymal transition (EMT) endoplasmic reticulum (ER) stress, but mechanism remains unclear. In our previous research, we found miR‐4756 levels were increased in urinary extracellular vesicles type 2 diabetes mellitus patients with macroalbuminuria. a preliminary study, may be derived from cells, its role has not been elucidated....
Background . NADPH oxidase 4 (NOX4) plays a major role in renal oxidative stress of diabetic kidney disease (DKD). NOX4 was significantly increased Egr1-expressing fibroblasts, but the relationship between Egr1 and DKD is unclear. Methods. For evaluation potential NOX4, both were detected HFD/STZ-induced mice HK-2 cells treated with TGF- β 1. Then, changes expression overexpression knockdown Egr1. The direct explored via chromatin immunoprecipitation (ChIP). Results. We found levels Egr1, α...
Objectives. Vitamin D deficiency plays a role in insulin resistance and the pathogenesis of type 2 diabetes mellitus. Little information is available about association between vitamin status Chinese population. Currently, evaluated by concentrations serum 25-hydroxyvitamin [25(OH)D]. This study explores relationship patients with Subjects Methods. included 117 diabetes. The following variables were measured: [25(OH)D], glycosylated hemoglobin A1c (HbA1c), fasting blood glucose (FBS), (FINS),...
During diabetic kidney disease (DKD), ectopic ceramide (CER) accumulation in renal tubular epithelial cells (RTECs) is associated with interstitial fibrosis and albuminuria. As RTECs are primarily responsible for energy metabolism, their function intimately linked to mitochondrial quality control. The role of CER synthesis the progression has not been thoroughly investigated. In this study, we observed a significant upregulation synthase 6 (Cers6) expression cortex db/db mice, coinciding...
The goal of this study was to investigate the possible protective effects sitagliptin against dyslipidemia-related kidney injury in apolipoprotein E knockout (apoE−/−) mice. Eight-week-old male apoE−/− mice were randomized receive either a high fat diet (HFD, group) or HFD mixed with (sita + for 16 weeks. A control group age- and gender-matched C57BL/6J fed HFD. exhibited increases body weight serum lipid levels addition high-density lipoprotein, 24-h urinary 8-hydroxy-2-deoxyguanosine...