A5085801720- Biotin and Related Studies
- bioluminescence and chemiluminescence research
- RNA and protein synthesis mechanisms
- Cellular transport and secretion
- Cellular Mechanics and Interactions
- Click Chemistry and Applications
- Microtubule and mitosis dynamics
- Bariatric Surgery and Outcomes
- Cell Image Analysis Techniques
- Single-cell and spatial transcriptomics
- Protein Degradation and Inhibitors
- PI3K/AKT/mTOR signaling in cancer
- Artificial Intelligence in Healthcare and Education
- Hippo pathway signaling and YAP/TAZ
- Retinal Imaging and Analysis
- Computational Drug Discovery Methods
- RNA Research and Splicing
- Heavy Metal Exposure and Toxicity
- Body Contouring and Surgery
- Advanced Proteomics Techniques and Applications
- Trace Elements in Health
- Acute Ischemic Stroke Management
- Cancer Genomics and Diagnostics
- Genetics, Bioinformatics, and Biomedical Research
- Magnesium in Health and Disease
The University of Sydney
2025
Vision Eye Institute
2025
University of Toronto
2014-2023
Lunenfeld-Tanenbaum Research Institute
2014-2023
Mount Sinai Hospital
2014-2023
Sinai Health System
2020-2022
SLAC National Accelerator Laboratory
2022
Stanford University
2022
Sinai Hospital
2022
Canada Research Chairs
2021
Abstract Viral replication is dependent on interactions between viral polypeptides and host proteins. Identifying virus-host protein can thus uncover unique opportunities for interfering with the virus life cycle via novel drug compounds or repurposing. Importantly, many viral-host take place at intracellular membranes poorly soluble organelles, which are difficult to profile using classical biochemical purification approaches. Applying proximity-dependent biotinylation (BioID) fast-acting...
Cell surface proteins have a wide range of biological functions, and are often used as lineage-specific markers. Antibodies that recognize cell antigens widely research tools, diagnostic markers, even therapeutic agents. The ability to obtain broad protein profiles would thus be great value in fields. There however currently few available methods for high-throughput analysis large numbers proteins. We describe here flow cytometry (HT-FC) platform rapid 363 antigens. Here we demonstrate HT-FC...
ABSTRACT Compartmentalization is a defining characteristic of eukaryotic cells, partitioning cellular processes into discrete subcellular locations. High throughput microscopy 1 and biochemical fractionation coupled with mass spectrometry 2–6 have helped to define the proteomes variety organelles macromolecular structures. However, many other intracellular compartments remained refractory such approaches, due for example difficulty in purifying non-membrane bound Proximity-dependent...
Apoptotic death of cells damaged by genotoxic stress requires regulatory input from surrounding tissues. The C. elegans scaffold protein KRI-1, ortholog mammalian KRIT1/CCM1, permits DNA damage-induced apoptosis in the germline an unknown cell non-autonomous mechanism. We reveal that KRI-1 exists a complex with CCM-2 intestine to negatively regulate ERK-5/MAPK pathway. This allows KLF-3 transcription factor facilitate expression SLC39 zinc transporter gene zipt-2.3, which functions sequester...
Abstract Purpose Large language models have shown promise in answering questions related to medical conditions. This study evaluated the responses of ChatGPT-4 patient-centred frequently asked (FAQs) relevant age-related macular degeneration (AMD). Methods Ten experts across a range clinical, education and research practices optometry ophthalmology. Over 200 patient-centric FAQs from authoritative professional society, hospital advocacy websites were condensed into 37 four themes:...
Epithelial cells can become polyploid upon tissue injury, but mechanosensitive cues that trigger this state are poorly understood. Using α-catenin (α-cat) knock-out Madin Darby Canine Kidney (MDCK) reconstituted with wild-type and mutant forms of α-cat as a model system, we find an established actin-binding domain unfolding designed to reduce force-sensitive binding F-actin (α-cat-H0-FABD
Understanding dynamic subcellular organization in living cells is key to unravelling mechanisms of intracellular signaling that goes awry disease. Using proximity-dependent biotinylation (BioID), we first established a reference map for the human cell at steady state (Go et al., Nature, 2021; humancellmap.org) serves identify protein baits can report on recruitment proteins selected organelles or structures. These BioID "sensors" then be applied look changes signaling. For example,...
Cryogenic electron microscopy (cryoEM) has been advanced to resolve atomic structures of biochemically purified macromolecules with details equivalent X-ray crystal structures.A unique aspect cryoEM is use image processing methods sort out images particles heterogeneous compositions and conformations.These capabilities have demonstrated across a spectrum including viruses, membrane channels, protein folding machines without substrates, RNA.The reliability these can be assured by using...