A5085985782- Enzyme Catalysis and Immobilization
- Electrochemical sensors and biosensors
- HIV/AIDS drug development and treatment
- biodegradable polymer synthesis and properties
- Synthesis and biological activity
- Synthesis of Indole Derivatives
- DNA and Nucleic Acid Chemistry
- Sulfur-Based Synthesis Techniques
- HIV Research and Treatment
- Peptidase Inhibition and Analysis
- Protein purification and stability
- Microbial Metabolic Engineering and Bioproduction
- RNA Interference and Gene Delivery
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Analytical Chemistry and Chromatography
- Quinazolinone synthesis and applications
Universidad Autónoma de Madrid
2016-2024
Consejo Superior de Investigaciones Científicas
2021-2024
Centro de Biología Molecular Severo Ochoa
2022-2024
Instituto de Catálisis y Petroleoquímica
2016-2017
Lipases from Candida antarctica (isoform B) and Rhizomucor miehei (CALB RML) have been immobilized on octyl-agarose (OC) further coated with polyethylenimine (PEI) dextran sulfate (DS). The enzymes just OC supports could be easily released the support using 2% SDS at pH 7, both intact or after thermal inactivation (in fact, most enzyme molecules were already desorbed). coating PEI DS greatly reduced release during improved stability. However, OC-CALB/RML-PEI-DS, full of to reuse required...
Lipase from Thermomyces lanuginosus (TLL) has been covalently immobilized on heterofunctional octyl-vinyl agarose. That way, the enzymes will have identical orientation. Then, it blocked using hexyl amine (HEX), ethylenediamine (EDA), Gly and Asp. The initial activity/stability of different biocatalysts was very different, being most stable biocatalyst with Gly. These had utilized to analyze if enzyme activity could decrease differently along thermal inactivation courses depending substrate...
Lipase B from Candida antarctica (CALB) immobilized on octyl-agarose (OC) was submitted to coating with polyethylenimine (PEI) and dextran sulfate (DS). Using lowly loaded enzyme preparations, the properties of OC-CALB preparations hardly improved, suggesting too large distance between molecules. However, using maximum loading, CALB stability greatly improved in different conditions after PEI coating. Moreover, release OC support presence detergents, or during thermal organic solvent...
Viral infections represent a major threat to human health and the global economy; however, most of currently available antiviral drugs are not fully effective in restricting viral replication selecting for drug-resistant variants. Targeted protein degradation technologies promising strategies avoid or delay emergence drug resistance. Among degradation-based multi-specific approaches, proteolysis targeting chimera (PROTAC) is main strategy applied field. In this review we will introduce...
Coupled with PCR, reverse transcriptases (RTs) have been widely used for RNA detection and gene expression analysis. Increased thermostability nucleic acid binding affinity are desirable RT properties to improve yields sensitivity of these applications. The effects amino substitutions in the RNase H domain were tested an engineered HIV-1 group O RT, containing mutations K358R/A359G/S360A devoid activity due presence E478Q (O3MQ RT). Twenty mutant RTs Lys or Arg at positions interacting...