A5041772508- Legume Nitrogen Fixing Symbiosis
- CRISPR and Genetic Engineering
- Plant nutrient uptake and metabolism
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Insect behavior and control techniques
- Bacterial Genetics and Biotechnology
- Insect symbiosis and bacterial influences
- Insect Utilization and Effects
- Insect and Pesticide Research
- Antibiotic Resistance in Bacteria
- Evolution and Genetic Dynamics
- Plant and animal studies
- Plant Disease Resistance and Genetics
- Gut microbiota and health
- Vector-borne infectious diseases
- Microbial Community Ecology and Physiology
- Yersinia bacterium, plague, ectoparasites research
- Actinomycetales infections and treatment
- Plant responses to water stress
- Insect and Arachnid Ecology and Behavior
The University of Texas at Austin
2017-2024
University of California, Davis
2014-2021
Plant (United States)
2015-2021
The phylogeny of the class Actinobacteria remains controversial, essentially because it is very sensitive to choice dataset and phylogenetic methods. We used a test proposed recently, based on complete genome data, which chooses among candidate species phylogenies number lateral gene transfers (LGT) needed explain diversity histories trees for set genomes. 100 completely sequenced genomes representing 35 families 17 orders evaluated eight different hypotheses their phylogeny, including one...
Plants within the Nitrogen-fixing Clade of Angiosperms form root nodule symbioses with nitrogen-fixing bacteria. Actinorhizal plants (in Cucurbitales, Fagales, Rosales) actinobacteria Frankia while legumes (Fabales) proteobacterial rhizobia. Flavonoids, secondary metabolites phenylpropanoid pathway, have been shown to play major roles in legume symbioses: as signal molecules that turn trigger rhizobial nodulation initiation signals and acting polar auxin transport inhibitors, enabling a key...
The bacterium Acinetobacter baylyi is a model organism known for its extreme natural competence and metabolic versatility. It capable of transforming environmental DNA at high frequency across all growth phases. type strain ADP1 was created by random mutagenesis precursor strain, BD4, to prevent it from forming cell chains in culture. has since been distributed between research groups over several decades acquired subsequent mutations during this time. In study we compare the genome...
Naturally competent bacteria can be engineered into platforms for detecting environmental DNA. This capability could used to monitor the spread of pathogens, invasive species, and resistance genes, among other applications. Here, we create Acinetobacter baylyi ADP1-ISx biosensors that detect specific target DNA sequences through natural transformation. We tested strains with sensors consisted either a mutated antibiotic gene (TEM-1 bla or nptII ) counterselectable flanked by from fungus...
Genus Frankia is comprised primarily of nitrogen-fixing actinobacteria that form root nodule symbioses with a group hosts known as the actinorhizal plants. These plants are evolutionarily closely related to legumes nodulated by rhizobia. Both host groups utilize homologs nodulation genes for root-nodule symbiosis, derived from common plant ancestors. The corresponding endosymbionts, and rhizobia, however, distantly bacteria, leading questions about their symbiotic mechanisms evolutionary...
We dekaryotized the multinucleate fungus Leucocoprinus gongylophorus, a symbiotic cultivated vegetatively by leafcutter ants as their food. To track genetic changes resulting from dekaryotization (elimination of some nuclei multinuclear population), we developed two multiplex microsatellite fingerprinting panels (15 loci total), then characterized allele profiles 129 accessions generated treatment. Genotype confirmed loss expected fungus. found no evidence for haploid and single-nucleus...
Animals form complex symbiotic associations with their gut microbes, whose evolution is determined by an intricate network of host and environmental factors. In many insects, such as Drosophila melanogaster, the microbiome flexible, environmentally determined, less diverse than in mammals. contrast, mammals maintain multispecies consortia that are able to colonize persist gastrointestinal tract. Understanding evolutionary ecological dynamics microbes different hosts challenging. This...
Due to their universal presence and high sequence conservation, ribosomal RNA (rRNA) sequences are used widely in phylogenetics for inferring evolutionary relationships between microbes metagenomics analyzing the composition of microbial communities. Most genomes encode multiple copies rRNA genes supply cells with sufficient capacity protein synthesis. These typically undergo concerted evolution that keeps identical, or nearly so, due gene conversion, a type intragenomic recombination...
Abstract Organelles and endosymbionts have naturally evolved dramatically reduced genome sizes compared to their free-living ancestors. Synthetic biologists purposefully engineered streamlined microbial genomes create more efficient cellular chassis define the minimal components of life. During natural or streamlining, deletion many non-essential genes in combination often reduces bacterial fitness for idiosyncratic unknown reasons. We investigated how what extent laboratory evolution could...
Organelles and endosymbionts have naturally evolved dramatically reduced genome sizes compared to their free-living ancestors. Synthetic biologists purposefully engineered streamlined microbial genomes create more efficient cellular chassis define the minimal components of life. During natural or streamlining, deletion many non-essential genes in combination often reduces bacterial fitness for idiosyncratic unknown reasons. We investigated how what extent laboratory evolution could overcome...
Abstract Bacterial biofilms are stable multicellular structures that can enable long term host association. Yet, the role of in supporting gut mutualism is still not fully understood. Here, we investigate Snodgrassella alvi , a beneficial bacterial symbiont honey bees, and find biofilm formation required for its colonization bee gut. We constructed fifteen S. mutants containing knockouts genes known to promote with putative roles formation. Genes included staA staB encoding trimeric...
Genus Frankia is comprised primarily of nitrogen-fixing actinobacteria that form root nodule symbioses with a group hosts known as the actinorhizal plants. These plants are evolutionarily closely related to legumes, which nodulated by rhizobia. Both host groups utilize homologs nodulation genes for root-nodule symbiosis, derived from common plant ancestors. However corresponding endosymbionts, and rhizobia, distantly bacteria, leading questions their symbiotic mechanisms evolutionary...
We report the genome sequence of Frankia sp. strain ArI3, recovered as a single contig from one run Oxford Nanopore Technologies (ONT) MinION instrument. The has G+C content 72%, is 7,541,222 bp long, and contains 5,427 predicted protein-coding genes.
ABSTRACT Due to their universal presence and high sequence conservation, rRNA sequences are used widely in phylogenetics for inferring evolutionary relationships between microbes metagenomics analyzing the composition of microbial communities. Most genomes encode multiple copies ribosomal RNA (rRNA) genes supply cells with sufficient capacity protein synthesis. These typically undergo concerted evolution that keeps identical, or nearly so, due gene conversion, a type intragenomic...
ABSTRACT Animals form complex symbiotic associations with their gut microbes, whose evolution is determined by an intricate network of host and environmental factors. In many insects, such as Drosophila melanogaster , the microbiome flexible, environmentally less diverse than in mammals. contrast, mammals maintain multispecies consortia that are able to colonize persist gastrointestinal tract. Understanding evolutionary ecological dynamics microbes different hosts challenging. This requires...